雜交帶 的英文怎麼說

中文拼音 [zájiāodài]
雜交帶 英文
zone of hybridization
  • 雜 : Ⅰ形容詞（多種多樣的; 混雜的） miscellaneous; varied; sundry; mixed Ⅱ動詞（混合在一起; 攙雜） mix; blend; mingle
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  1. By using these antibodies, which were raised to immunoreact with total proteins of purified mitochondria from different organs of mung bean seedlings, we find that there were two hybridizable aox bands in mitochondria in mung bean seedlings. their molecular weight was about 35kd and 38kd respectively

    將此抗體與來自綠豆幼苗不同器官線粒體的總蛋白分別進行western雜交，可觀察到綠豆幼苗線粒體中具有兩條清晰的aox雜交帶，它們的分子量分別為35kd和38kd 。
  2. 2. 5 ul of 10 x reaction buffer, 1. 5 ul 25mm mgcl2, 0. 3 ul lomm dntp, 0. 5 ul taqdna polymerase ( 5 u / ul ), and lul ( = 20ng ) of primer were used in per reaction. each reaction was overlaid with one drop of paraffin oil. the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec, annealed at 37 1 min, extended at iv b 72 for 2 min and repeated the cycle 45 times, at last, extended at 72 ' c for lomin

    等（ 1995 ）利用rapd標記區分美國東部一雜交地帶的蟋蟀的兩個姐妹種， allonemobiusfasciatus和a . socius ，並於1998年使用rapd和異型酶標ic做出了這兩種蟋蟀的基因連鎖圖；國內田英芳、鄭哲民（ 20of ）首次將rapd技術運用於蟋蟀總科的分子系統學研究中，採用2種引物對7種蟋蟀進行了基困組dna多態性研究，並應用upg問a法構建樹狀圖，椎測系統發生關系
  3. Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing, while a faint band only in muts recombinant after 72 hours. western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced. anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them

    結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。
  4. To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7

    為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
  5. There are mainly three arguments on the physical origin of the new conduction band minimum of ganas ( or galnnas ). they are band anti - crossing model, gaas conduction band mixing model and impurity band model

    關于ganas的導帶帶邊的物理本質，目前主要有三種爭論較激烈的觀點，它們分別是能帶反交叉模型， gaas導帶混合模型，雜質帶模型。
  6. The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn

    用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。
  7. Hybridization bands were detected by southern blot analysis using lea3 gene probe labeled by digoxigenin the result showed that foreign lea3 gene integrated into strawberry genome

    2 。對點雜交為陽性的植株進行southernblot分析，轉化植株檢測到了雜交帶，除預期的1
  8. During wheat and barley bred in winter in sanya, some technological characteristics concerned must be wielded according to the ecological conditions there. 1 ) carefully select materials and avoid planting the materials that can not head in sanya. 2 ) cultivated measure : spread lime before ploughing ; and make furrows during soil preparation. spread funandan in sowing furrows. after every irrigation and heavy rains, surplus water must be drained off immediately and soil must be intertilled in time. it is also a key measure in breeding in winter to prevent and eliminate the plant disease, pest and mice during plant growing period. 3 ) the flowering period of parent for hybridization must be adjusted. 4 ) the criterion for each trait selection to breeding materials should be soften to different degrees

    在三亞冬繁麥類時，應根據當地生態條件掌握好有關技術特點：慎重挑選冬繁材料，盡量避免攜帶可能在三亞不抽穗的材料；栽培措施應注意耕翻前撒施石灰，整地需開廂起壠，播種溝先撒呋喃丹，灌水及大雨後要排除余水，及時鬆土，生育期間注意防治病蟲鼠害；雜交親本應注意調節花期；對育種材料各性狀的選擇標準都要不同程度地放寬。
  9. Scientists from the international crops research institute for the semi - arid tropics ( icrisat ) are developing sorghum varieties and hybrids that have higher amount of sugar - rich juice in their stalks for mozambique

    國際半乾旱地區熱帶作物研究所（ icrisat ）的科學家們正為莫三比克研究高粱的變種以及雜交品種，這些品種在它們的莖干汁液中富含這大量的糖分。
  10. Was first processed by dgd embedment and embedment - free technique and general technique for em morphology. a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed. then using western blot assay, we found a lamin - like component band of 68kd protein in the three - step - fractionated cells. to investigate the distribution of the lamin - like protein in cells, a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes. the results revealed that the positive reactivity presented at different part of the cells. the perinuclear cross - actions were distinct, and cross - action with the oral apparatus and the cortex were also obtained

    本文以dgd包埋去包埋技術對草履蟲的核纖層通過透射電鏡和免疫熒光分子雜交等技術進行了觀察。結果顯示，在核周存在由10nm纖維組成的核纖層免疫熒光結果表明，在核周呈陽性反應，並在其表皮口器等部位呈交叉的陽性反應蛋白分子雜交的反應帶在68kd處呈陽性反應。
  11. The analyses showed that rapd was able to differentiate mainly at the species level, while eric is effective at the strain level

    以黑木耳菌株為探針進行southem雜交，該探針與黑木耳和琥珀木耳均有雜交帶，而毛木耳沒有。
  12. Most of our production however is concentrated on the wide plainsofemilia romagna extremely suitable for direct sowing and mechanised farming techniques ? and the windy, sunny hills of the marches, south of emilia romagna, idealfortheproduction of hybrid onions and hybrid cabbages

    但公司最主要的生產基地集中在中部艾米利亞-羅馬涅大區適應農業機械化的直接播種耕作的寬闊平坦地帶和南部馬爾凱大區多風和日照陽光充裕的丘陵地帶，該地帶是生產甘藍類和各類雜交洋蔥類種子的理想之地。
  13. Sds - page showed efficient expression and secretion of the rhfl. the highest yield ( 108 mg / l ) was obtained when expression was induced with 0. 5 % methanol for 96 hours

    Western雜交實驗出現了可被抗體特異性識別的雜交帶，證實此條帶即為重組畢赤氏酵母km7lppicgkil分泌表達的重組fl蛋白。
  14. In this construct, hf2 dna should be expressed under the control of the camv 35s promoter. the construct was transformed to petunia hybrida of the light pink via agrobacterium tumefaciens eh a105 using the leaf disc method. in the end, three transgenetic plants were obtained by screening with the phosphinothricin resistance and pcr amplification

    通過三親交配將重組質粒pc3301 - hf2導入根癌農桿菌eha105 ，抗性篩選、 pcr檢測及dna點雜交表明轉化后的農桿菌帶有完整目的基因片段，能夠用於轉化植物。
  15. 27kd protein bands were detected in transgenic plants by western blot analysis, and the results showed that the foreign lea3 gene could express in transgenic strawberry plants

    Westernblot分析，在轉化植株中檢測到了27kd雜交帶，表明在轉化草莓體內有外源基因翻譯產物的積累。
  16. Except for 1. 0 kb hybridization band, other different hybridization bands were also detected which demonstrated that lea3 gene integrated into strawberry genome in different loci or copies

    Okb雜交帶外，還有其他片段人小不等的雜交帶。不同的株系形成不同的雜交譜，表明這些轉化植株來自不同的轉化細胞。
  17. The genomic dna extracted from three rubber tree strains ( pr107, rrim600, gt1 ) was digested with hinckr. the southern blot was made with the same probe as above. the result showed that all the three strains turned up only one brand ( - - 4. 8kb ). the three strains had the same inherent background

    用橡膠樹pr107 、 rrim600 、 gti三個品系的基因組洲a分別經hinan酶切后，進行了southern雜交。結果表明，三個無性系都只出現一條雜交帶，位置一』致（ 4
  18. I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

    從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。
  19. Genetic markers enable plant breeders to begin their selection at the cell stage by looking for the gene responsible and checking at an early stage of cross - breeding, whether the desired gene has been inherited by the offspring plant, instead of having to go through the process of cross - pollination and then growing the offspring to see if they have inherited the desired characteristics, and if not, starting all over again.

    遺傳標記物的存在能夠使植物育種家在細胞階段就開始進行選種，所用的方法包括尋找相應的基因以及在雜交早期進行檢查看看想要的基因是否遺傳到子代植物，這樣就不需要經過復雜的傳統育種過程：雜交授粉種植子代觀察子代是否獲得想要的性狀如果子代沒有攜帶想要的性狀，再從頭開始
  20. The nature of the mutant alleles is either nonsense mutation or the mutation which disrupted the splicing of the primary transcripts. anti - en antibody staining and in situ hybridization on sll germline clone ( glc ) embryo using wg antisense probe showed defective en protein bands and defective wg transcripts bands

    通過對sll生殖系克隆胚胎進行的抗engrailed （ en ）抗體染色和wg反義探針的原位雜交結果表明，與野生型果蠅的胚胎相比較， en蛋白和wg轉錄物條帶出現了明顯的缺失。
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