雜交抗體技術 的英文怎麼說
中文拼音 [zájiāokàngtǐjìshù]
雜交抗體技術
英文
hybrid antibody technic- 雜 : Ⅰ形容詞(多種多樣的; 混雜的) miscellaneous; varied; sundry; mixed Ⅱ動詞(混合在一起; 攙雜) mix; blend; mingle
- 交 : Ⅰ動詞1 (把事物轉移給有關方面) hand over; give up; deliver 2 (到某一時辰或季節) reach (a cert...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 體 : 體構詞成分。
- 技 : 名詞(技能; 本領) skill; ability; trick; technique
- 術 : 術名詞1. (技藝; 技術; 學術) art; skill; technique 2. (方法; 策略) method; tactics 3. (姓氏) a surname
- 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
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However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting
利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法,但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性,利用特異性的豌豆鐵蛋白抗血清,就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測,從而更好地進行轉基因方面的研究。It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions, clearing aged cells and metabolic products, regulating immune responses and protecting against infection. in some pathological states such as psoriasis and contact dermatitis, a certain serum level of the antibody could inhibit the progression of the diseases, and is beneficial to the recovery from the diseases. after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies, meanwhile, experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) ", a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability, immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis, and recombinant antibody is under investigating
抗角蛋白自身抗體( akautoab )是naa的重要組成部分,以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ,並對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究,直接或間接地發現akautoab是機體正常免疫調節網路的組成部分,在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用;在某些病理情況下(如銀屑病、接觸性皮炎等) ,體內akautoab的組分和滴度會發生變化,而正常水平的akautoab則有利於限制病情的發展,促進損傷的修復。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術,從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ,克隆抗體重鏈和輕鏈可變區基因,加入連接肽引物( linkerprimer )組裝成單鏈抗體scfv ( singlechainfragmentvariable )基因並用rs引物進行擴增, sfi 、 not酶切,回收后與pcantab5e載體相連,轉化e . colitg1宿主菌,構建單鏈抗體文庫。1975 hybridoma technology deeloped for production of monoclonal antibodies
1975年,雜交瘤技術被應用於生產單克隆抗體1975 hybridoma technology developed for production of monoclonal antibodies
1975年,雜交瘤技術被應用於生產單克隆抗體Unique data exchange random noise technology can effectively antagonize the analysis of logic analyzer and attacks of various debug tool and disallow the simulation of data by software emulator programs
採用獨創的資料交換隨機雜訊技術,有效對抗邏輯分析儀及各種調試工具的攻擊,完全禁止軟體模擬程式類比的資料。分享友人