雜交瘤 的英文怎麼說
中文拼音 [zájiāoliú]
雜交瘤
英文
hybidoma-
2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6. their antibodies " isotypes are tested by goad anti - mouse isotype regent
取馴化好並處于對數生長期的2 - e _ 4 - a和s _ 2 - b細胞，再常規融合和篩選，三次克隆化后得穩定分泌雙特異性抗體的雜交-雜交瘤細胞株6株。The ascites fliud titer of the other hybridoma s2 for further hybridization is 1 : 16000 by blood agglutination. the isotype of 2 - e4 is igg2a, and s2 is iggs
用於再雜交的另一細胞株為本室保存的分泌抗人a型紅細胞單克隆抗體的雜交瘤細胞株s ； ，其血凝效價為l ： 16000 。Guan xiaohong ( 1991 ) established a cell line of the monoclonal anti - idiotypic antibody ( anti - id ) np30 of schistosoma - 6 - japonicum, whose isotype was identified to be igm and which was testified to be internal image of gaa
分別以日本血吸蟲單克隆抗獨特型抗體wbo的雜交瘤細胞株總基因組dna和其提取rna進行rticr合成的cdna第一鏈為模板，擴增v 。 、 v 。Preparation and characterization of monoclonal antibody against botulinum neurotoxin type b
型肉毒毒素單克隆抗體雜交瘤株的建立Serious cross reation existed between v. albo - atrum and mv2, mv3, mv4. the other pathogen isolates v31 and v32 also had cross reactions, but the reaction was not serious. because limited number of pathogen isolates were selected, it could not prove that the selected immunogen was widely presentative, more pathogens isolates should be tested to verify the acquired hybridomas cells
5株單抗雜交瘤細胞中沒有一株具有種或屬的特異性，其中mv2在棉花黃萎病菌若干菌系間的檢測表明其能夠區分不同的致病類型； mv1和mv4組合檢測的結果基本上能將棉花大麗輪枝菌鑒定到種；黑白輪枝菌與mv2 ， mv3 ， mv4的交叉反應比較強烈，其他菌株v3 ， v32有個別的交叉反應，但不強烈Frankfurt os, robb ja, sugar baker ev, et al. monoclonal antibody tosingle2stranded dna is specific and sensitive cellular marker of apoptosis [ j. exp cell res, 1996, 226 ( 2 ) : 3872397
吳國慶，李少華，徐志偉，等.抗單鏈單克隆抗體雜交瘤細胞株的建立及鑒定[ j ] .細胞與分子免疫學雜志， 2002 ， 18 （ 5 ） : 4792480Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody
選其中一株3 - h _ （ 11 ）做雜交-雜交瘤細胞染色體計數，同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數：制備腹水型和上清型抗體，用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體，即紅細胞固相吸附法測雙特異中文摘要性抗體，紅細胞凝集試驗測單特異性抗人a型紅細胞抗體，間接elisa法測抗p24抗體；用腹水型抗體做耐熱性及耐凍融實驗。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions, clearing aged cells and metabolic products, regulating immune responses and protecting against infection. in some pathological states such as psoriasis and contact dermatitis, a certain serum level of the antibody could inhibit the progression of the diseases, and is beneficial to the recovery from the diseases. after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies, meanwhile, experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) ", a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability, immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis, and recombinant antibody is under investigating
抗角蛋白自身抗體（ akautoab ）是naa的重要組成部分，以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，並對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究，直接或間接地發現akautoab是機體正常免疫調節網路的組成部分，在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用；在某些病理情況下（如銀屑病、接觸性皮炎等） ，體內akautoab的組分和滴度會發生變化，而正常水平的akautoab則有利於限制病情的發展，促進損傷的修復。The batch culture of jal1 hybridoma cells
1雜交瘤細胞的批量培養研究The ig class is iggi. mcab of at - iii provides very efficient tools for the detection and purification of at - iii
獲得三株分泌抗人抗凝血酶的雜交瘤細胞株d5 、 e2 、 f6 ，免疫球蛋白的類型為igg _ 1型。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。1975 hybridoma technology deeloped for production of monoclonal antibodies
1975年，雜交瘤技術被應用於生產單克隆抗體1975 hybridoma technology developed for production of monoclonal antibodies
1975年，雜交瘤技術被應用於生產單克隆抗體Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months. the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa. through three clonings less diversed anti - p24 hybridoma cells are gained
方法：基因工程p24抗原免疫小鼠4次，歷時2個月，取脾細胞與骨髓瘤細胞株sp2 0 ，用peg融合， hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞，三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。Establishment and characterization of two hybridoma cell lines secreting anti - osteosarcoma antibody
抗人成骨肉瘤雜交瘤細胞系的建立及其特性鑒定Expression of human papillomavirus type 16 l1 and construction of hybridoma cell strain of human papillomavirus type 16 l1 monoclonal antibody
1蛋白表達及其單克隆抗體雜交瘤細胞株的建立The screening of supernatants was carried out with using indirect elisa. after subcloning by limiting dilution and selection. five clones of cells with high od value were acquired and named mvi
過陽性雜交瘤細胞的篩選和有限稀釋法的克隆化步驟，獲得了5株od值高的單克隆細胞株，編號為mv1 ， mv2 ， mv3 ， mv4 ， mv5 。H chain cdr3 region is thought to be the antigenic epitope of antibody of monoclonal antibody np30. therefore, anti - id antibody vaccine and antigenic epitope vaccine are important strategy in developing schistosomajaponicum vaccine
Fv基因，且其表達產物保留了抗體的親和性和特異性，有可能替代雜交瘤細胞分泌的單克隆抗體np30作為免疫檢測試劑。分享友人