雜交篩選 的英文怎麼說

中文拼音 [zájiāoshāixuǎn]
雜交篩選 英文
screening by hybridization
  • 雜 : Ⅰ形容詞（多種多樣的; 混雜的） miscellaneous; varied; sundry; mixed Ⅱ動詞（混合在一起; 攙雜） mix; blend; mingle
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 篩 : 名詞[書面語] （植物名） sedge
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  • 篩選 : dressing by screening; screen; preparation by screening; preparation; choose by means of a sift; ...
  1. Even so, by truncating hbv pres gene, we finally obtained some useful " " bailors ", either nontoxic or self - activating, and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library

    我們通過第回軍巨大學碩士學位論文對pres基因分段截短的方法，獲得了對酵母細胞即無毒性作用，又沒有自激活作用的「誘餌」 ，通過它在酵母雙雜交系統中篩選構建於ad載體的人胎肝。
  2. 2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6. their antibodies " isotypes are tested by goad anti - mouse isotype regent

    取馴化好並處于對數生長期的2 - e _ 4 - a和s _ 2 - b細胞，再常規融合和篩選，三次克隆化后得穩定分泌雙特異性抗體的雜交-雜交瘤細胞株6株。
  3. In our study, the igii subdomain of trka extracellular domain was fused to pgbkt7 as a bait to screen yeast random peptide library which contains 1 107 independent random peptide clones

    我們將trk受體膜外域1911結構域與gal4蛋白融合構建成pgbkt7一ign ，確認無自激活作用后，將其作為誘餌蛋白，洲于酵母雙雜交篩選。
  4. To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7

    為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
  5. In order to figure out the machanism of transcription control by ap - 2 a and to find the partner proteins which derectly binding to ap - 2 a, a yeast two - hybrid system by using ap - 2 a as a bait was performed to screen the hela cell cdna library

    為了闡明ap - 2確切的轉錄調控方式，找出與其直接相互作用的蛋白質因子，我們以ap - 2為餌蛋白通過酵母雙雜交對hela細胞的cdna文庫進行了篩選。
  6. For the complexity and diversity of the black - spot cause of formation, we find a method based on the rough set to determine the most important inducement of the black - spot, then we can repair the inducement in effect and save more time and money

    該文針對交通事故多發點成因的復雜性和多樣性，提出通過粗集來對公路交通中的不利因素進行篩選，找到形成事故多發點的最大誘因，從而有針對性地進行整治，能夠有效地節約時間和費用，避免不必要的人力、物力浪費。
  7. Four c - ch3, six aromatic carbons, six carbons of mycosamine were the characteristics of candicidin d, one ketal corresponding to mycosamine and four ketons indicated that no hemiketal formed between c - 15 and c - 19 in fr - g08b or candicidin d. however, such hemiketal was usually thought having been formed

    用這個探針，對鏈黴菌fr - 008的基因文庫進行雜交篩選，獲得了3個陽性克隆，經southern雜交分析，發現它們均含有6 . 4kb共同的陽性片斷。
  8. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  9. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  10. In this study, sd rats were used to establish the animal model of brain injury induced by repeated + gz exposure and suppression subtractive hybridization technique was adopted to screen the differentially expressed genes in rat brains of + gz exposure group. the aim of the study was to obtain preliminary experimental data for the molecular mechanisms of the brain injury

    本研究利用大鼠重復+ 10gz暴露引發腦損傷的動物模型，觀察腦的病理學改變；應用抑制性消減雜交技術篩選+ gz重復暴露大鼠腦的差異表達基因，旨在初步探討+ gz重復暴露致腦損傷的分子機制。
  11. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  12. Eight varieties of vitis amurensis rupr. ( v. t. r ) of intraspecific hybridization and interspecific hybridization were introduced into from 2001 ~ 2006, the results showed some which suited the temperature and soil condition to be the main varieties were selected from those varieties with cold - resistance and high yielding. " zuo youhong " was for dry - red wine, " hasang " was for fruit juice and " shuang you " and " shuang hong " were for adjusting color and acid

    摘要2001 ~ 2006年，引種8個山葡萄種內和種間雜交品種，篩選出適宜當地氣候和土壤條件、抗寒、產量高、可釀造干紅葡萄酒「左優紅」 、加工果汁飲料的「哈桑」 、用於調色和調酸的「雙優」和「雙紅」為當地主栽和推廣品種。
  13. In this paper, we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid

    以dig - oligo2為探針，菌落原位雜交篩選cosmid文庫，得到56個陽性重組子。
  14. Nor protein partner interacting with pgl29 was detected by yeast two - hybrid screening. thus further work needs to be made to understand the fu

    酵母雙雜交篩選結果，也未得到特異地與pgl29相互作用的直白質分于，因而現有的實驗無法確定該基因的功能。
  15. Selected one of the 14 strains - s93, s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18, then transformed into dh5 a. filtering the clone with hybridization in situ, a 1 kb frament clone has been cloned

    使用sau3ai對基因組dna進行不完全酶切，回收2 3kb片段，與puc18質粒連接轉化大腸桿菌，利用地高辛標記探針，使用菌落原位雜交篩選轉化子；篩選到包含有約1kb外源片段的轉化子。
  16. Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence

    以pcr擴增的人血清白蛋白（ hsa ）基因片段為探針，從人的基因組文庫中雜交篩選的陽性克隆中，經測序分析，有一個克隆含有全長hsadna ，用從其它的陽性克隆中選取兩種dna片段，即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ，與人hsadna一起，進行顯微共注射，成功制備了轉多基因小鼠。
  17. Pit13, an interactor binding to trpt1 protein, was screened by yeast two - hybrid, and confirmed by pull down and co - ip assays. the association of trpt1 with pit 13 demonstrated that trpt1 should probably participate in other activities besides in pre - trna splicing. more experiments will be required to determine the role of pit 13 protein

    0酵母以雜交篩選、加衛工加wn檢測及ip實驗均證明m卜1 」且與功能未知的pit13蛋； 」之間存在著相互作用，說明imj基因除了參與trna剪接之外，還可能具有其它方面的功能，有待進一步研究。
  18. Positive probe was made from the floral meristem materials cultured for 5 days and 10 days with hormones by rna reverse transcription and labelling with radioactive dctp ; two negative probes from uncultured explants and it cultured for 5 days and 10 days. then, we did calculating signal arrays, sequencing, sequence analysis and alignments on genebank etc. finally 229 different ests were got from the cdna library

    用在含有外源激素的培養基上培養5天（花分生組織開始形成）和10天（花分生組織已基本形成）的風信子外植體為材料，構建起cdna文庫。利用cdnamicroarrray技術，經過雜交篩選和est分析序列分析，最終從cdna文庫中獲得了229個不同的外源激素誘導響應的基因序列。
  19. In y2h, rapgap from c. elegans was used as bait to screen c. elegans cdna library. after - lth screen, 63 colonies were checked

    在酵母雙雜交系統中，將ppc97 rpg 」與ppc86七dna文庫共轉化入酵母菌y190細胞，經過一lth篩選后，有63個菌落擬為陽性菌落。
  20. In order to study the upstream and downstream components in g protein and calmodulin signal pathways, a yeast two - hybrid system was used to screen interaction partners of these two proteins

    本文利用酵母雙雜交系統分別篩選了與這兩種蛋白相互作用的蛋白質，以進一步研究g蛋白和cam信號轉導途徑中的其它上下游組分。
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