雞痘病毒 的英文怎麼說
中文拼音 [jīdòubìngdú]
雞痘病毒
英文
fowlpox virus-
Recombinant fowlpox viruses ( rfpvs ) coexpressing mdv gb and aiv ha gene were constructed by using different promoters of ps and p7. 5
2 、共表達aivha基因和ndvf基因的重組雞痘病毒的構建。In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法,將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞,經6輪蝕斑克隆、篩選、表達, pcr鑒定和dot - elisa檢測,證明該重組病毒已構建成功,並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。Vaccination trials were carried out in spf chickens, protection index ( pi ) against md5 + rbib challenge induced by 106 pfu rfpv - gb - ha was 53. 8 while pi against aiv challenge induced by 106 pfu rfpv - gb - ha was 69. 5
綜上所述,本論文嘗試了不同禽病的主要保護性抗原基因在雞痘病毒載體的聯合表達,為探索基因工程聯苗打下了基礎。Studies on the inherent stability of recombinant fowl pox virus expressing glycoprotein b of infectious laryngotracheitis virus
基因重組雞痘病毒疫苗的遺傳穩定性評價分享友人