雞胚培養 的英文怎麼說
中文拼音 [jīpēipéiyǎng]
雞胚培養
英文
chick embryo cultivation-
The a / pr / 8 / 34 ( hln1 ) adapted to mouse lung was propagated in allantoic cavity of 9 - day - old embryonated chicken eggs and titered by hemagglutinin test, and the tilers was 1 : 320
用9日齡雞胚培養人流感病毒的鼠肺適應株戶jp側8 / 34 （ hini ） ，通過血凝實驗測得病毒滴度為1 : 320 。Like many embryonic cells, myoblasts from prenatal rats or chicks are easy to culture.
正如許多胚胎細胞一樣，胚胎大鼠或雞的成肌細胞是易於培養的。Cultivation of avian encephalomyelitis virus in cef
利用雞胚成纖維細胞培養禽腦脊髓炎病毒的研究Propagation of virus in embryonating eggs was first used by goodpasture and woodruff in 1931.
1931年Goodpasture和Woodruff首次應用雞胚培養病毒。Materials and methods : fecal, cloacal and tracheal swabs from different types of poultry were collected in 6 live - bird retail markets once a week. they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 " c for 72 hours. hi and nl assays were performed to detect the subtype of viruses if ha test were positive ( ad ^ 16 ) and contamination test were negative
材料和方法：每星期收集一次標本，收集的標本常規處理， 9 - 11日齡雞胚尿囊腔接種， 35培養72小時，收取尿囊液，血凝陽性（ ad 16 ）且細菌培養陰性者- 70保存並進一步做hi ， ni實驗鑒定亞型。Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast
66弱毒株在雞胚成纖維細胞上的培養Effect of removal of external calcium on phosphoinositide hydrolysis in cultured myotubes of embry - onic chicken
去細胞外鈣對培養雞胚肌管磷脂酰肌醇水解的影響英文The information generated in current study suggests that the developing influenza ecosystem in southern china region may play an important role in the process of emerging novel influenza viruses, even directly impact the genesis of pandemic influenza strains. materials and methods : fecal, cloacal and tracheal swabs from different types of poultry were collected in live - bird retail markets once a week. they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 for 72 hours
本課題意在: 1 、以pa和cu為代表，探討hgnz亞型流感病毒在這些新形成的小種群中的流行情況; 2 、探討這些小種群中流感病毒的來源:是從其它動物跨種屬傳遞而來，還是本身為流感病毒的天然宿主: 3 、探討這些新形成種群中hgnz亞型流感病毒的進化情況，以及其在整個流感病毒生態體系中的作用;材料和方法:每周採集標本一次，常規處理后，接種於9一n日齡雞胚尿囊腔， 35恆溫培養72小時，收取雞胚尿囊液。Propagation of virus in embryonating eggs was first used by goodpasture and woodruff in 1931
1931年goodpasture和woodruff首次應用雞胚培養病毒。In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures
幼雞感染該病毒后，引起麻痹、頭頸震顫甚至共濟失調，而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響，鎂離子可增強病毒對熱的穩定性，病毒的浮密度為1 . 31g ml ，直徑為22 - 30nm ，該病毒主要在雞胚中增殖，在大多數細胞培養物中不生長。At primary culture, pgcs co - cultured with their gonadal stromall cells were well grown. when subculture, we used primary chicken embryonic fibroblast ( pcef ), primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells. forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines
繼代培養時，經過反復實驗比較雞胚原代成纖維細胞飼養層（ pcef ） 、小鼠原代成纖維細胞飼養層（ pmef ） 、 snl細胞飼養層等三種不同飼養層的作用效果，最終發現以雞胚原代成纖維細胞製作飼養層同時添加各種生長因子最適合雞類eg細胞的生長，但pcef與pmef之間的差異不顯著。The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates
加入新鮮的eg細胞培養液（含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分）培養24小時以後， pgcs開始部分貼附於共培養的生殖原基細胞上。Chick embryo cell culture
雞胚細胞培養The bmn cells and fifth instars were infected by the recombinant virus bacpak - angiostatin. the bioactivity of the protein product was determined by human umbilical vein endothelial cells ( ecv304 ) growth assay in vitro and inhibition of vascular growth assay in cam in vivo. angiostatin showed significantly inhibitory effect on endothelial cells
重組病毒以moi = 10感染家蠶細胞（ 2 10 ~ 6個細胞/ 5ml ）和家蠶5齡幼蟲，表達產物用體外培養的人臍靜脈血管內皮細胞（ ecv304 ）及體內雞胚尿囊膜（ cam ）新生血管實驗檢測其抑制活性： （一）血管抑素可明顯抑制體外培養的內皮細胞增殖。And cut open the symptom of examining and infer from the ill chicken ' s only appearance for mycoplasma gallisepticum infection, through isolating germs from the disease chicken, the separation that and then carry on the bacterium trains and check with the microscope, the inoculation agglutinate experiment of the whole blood of chicken ' s embryo, confnm as the mycoplasma gallisepticum infection at last
從患病雞只的外觀和剖檢癥狀推斷為支原體疾病，通過從病雞中分離病原微生物，進而進行分離培養和顯微鏡檢查，雞胚的接種、全血凝集實驗，最後確認為禽的支原體疾病。分享友人