離子對層析 的英文怎麼說
中文拼音 [líziduìcéngxī]
離子對層析
英文
ion-pairing chromatography- 離 : Ⅰ動詞1 (離開) leave; part from; be away from; separate 2 (背離) go against 3 (缺少) dispens...
- 子 : 子Ⅰ名詞1 (兒子) son 2 (人的通稱) person 3 (古代特指有學問的男人) ancient title of respect f...
- 對 : Ⅰ動詞1 (回答) answer; reply 2 (對待; 對付) treat; cope with; counter 3 (朝; 向; 面對) be tr...
- 層 : i 量詞1 (用於重疊、積累的東西 如樓層、階層、地層) storey; tier; stratum 2 (用於可以分項分步的...
- 析 : Ⅰ動詞1. (分開; 散開) divide; separate 2. (分析) analyse; dissect; resolve Ⅱ名詞(姓氏) a surname
- 離子 : [物理學] ion
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Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da
採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱,通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰,這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的,通過tricine - sds - page檢測,只能檢測到一條分子量相對較大的細菌素,分子量在8 , 570da左右。Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化,純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時,還呈現抗血小板聚集活性。This paper mainly discusses the designing and testing method to the dds acousto - optic mode locking. it also makes some further analysis on the critical technology - - - - - - the transducer acoustical membrane matching and transducer thinning, which can directly affect the performance of acousto - optic elements. it then analyses the heat effect of acousto - optic elements and the technology of transducer thinning by developing ion - beam sputtering of high frequency acousto - optic elements
本文重點討論了dds聲光鎖模器的設計及測試方法,討論和分析了影響聲光器件性能的關鍵工藝換能器聲學膜層匹配和換能器減薄工藝,對聲光器件的熱效應進行了測試分析,對離子刻蝕法聲光換能器減薄新工藝作了一定的探討。Abstract : this paper, by analyzing the effect of differences of mineral structure of 2 1 layer minerals, the properties of their interlayer charge and changes of some properties caused by weathering on the attractive force for interlayer cation, reached the conclusion that : the fixation capacity is determined by the ratio of the total amount of interlayer charge and that of tetrahedral charges. the relationship of interlayer cation fixation and octahedral structure of secondary minerals is probably different from that of primary minerals. the oxidation and reduction of variable valence cations can greatly influence the cation fixation capacity
文摘:分析2 1型礦物的結構差異、層間電荷性質以及在風化過程中某些性質的變化對層間陽離子吸引力的影響,對已有報道中一些矛盾的結果或不具普遍意義的結論進行了討論.得出幾個較為明確的結論:層間總電荷與四面體電荷所佔比例大小共同決定礦物對層間陽離子的固定能力;原生礦物中八面體結構與層間陽離子固定間的關系對次生礦物而言,也許並不一致;八面體中可變價陽離子的氧化還原,可導致礦物固定陽離子能力發生較大變化Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用線蟲誘導下owvt - 1菌株液體發酵,通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶,生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用,同時測定了該酶理化特性,酶活力在75附近酶活力最高,隨著ph的增加酶的穩定性升高,與膽堿酯酶具有相似的ph曲線,對特異性底物aape ( suc - ala - ala - pro - glu - pna )具有作用, ssi和ci - 2抑制該酶的活性。The ps ii native fractions ( 20 % and 30 % ) were loaded onto a deae column. the fraction eluted with 150 mm nacl was presented dcip reduction activity and was highly depleted in chi c and xanthophylls, and as such could be considered a ps ii core complex
對于有dcip光還原活性的20和30層帶的復合物,進一步deae離子交換層析純化。 150mmnacl洗脫純化后的樣品經過熒光激發光譜測定發現,已經去除了葉綠素c和墨角藻黃素,並且仍然具有dcip的光活性,分析是ps核心復合物。Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others
通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化,並得到電泳純的酶。Conentional methods such as fractionation of complex clinical samples by ionic exchange chromatography and new methods such as enriching low abundant proteins by affinity capture with a combinatorial library of ligands14 proide much needed tools for processing complex biological and clinical samples for proteomics research
傳統(如對復雜臨床樣品通過離子交換層析進行分離)和現代的方法(如通過親和捕獲與配體庫的組合富集低豐度蛋白)為處理用於蛋白質組學研究的復雜的生物學與臨床樣品提供了極需的工具。Mm ). mg2 +, mn2 +, zn2 +, fe2 +, fe3 +, cu2 + have enabled effect on enzyme activation and edta produce a strong inhibitory effect on the enzyme. embranch amino acid have no effect on the enzyme
在離子交換柱層析中,採用不同的ph值及不同類型的緩沖液對純化條件進行優化,最終選擇了ph6 . 0mes緩沖液,並得到了酶蛋白洗脫點為0 . 24 0 . 32mol lnacl 。In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization
本研究用改良后的親和層析法分離純化mr , lowry法測定其蛋白質濃度,在精子穿透試驗( spermpenetrationassay , spa )模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性;以已知濃度的pmr ( purifiedmannose - ligandreceptor )干預精子半透明帶試驗,觀察用pmr預處理半透明帶對精子與透明帶結合的影響。But these techniques can not provide physiological ph and ionic strength, and radio - iodination of heparin required for ace endangers health of human and environment. for avoiding the radiological hazards and reflecting directly the binding characteristic of heparin to protein
但親和層析柱易被污染失活, ace技術不能提供生理ph范圍和離子強度及ace技術必需的標記肝素的放射性物質對人和環境具有很大危害。The soi is of crystal quality and the box is uniform in thickness, with the interfaces of si / sioa / si smooth and sharp. we have systematically studied the dependence of the formed soi structure on the process parameters, such as ion energy, implantation dosage, substrate temperature, as well as the annealing temperature. with xtem, sims, srp, rbs, ir, raman, aes, xps and other characterization tools, it was found that a dose window at fixed energy for water plasma ion implantation to form high quality soi structure similar to the conventional simox process exists
本論文還系統地研究了不同注入劑量、注入能量、注入時基底溫度以及退火溫度對所形成soi結構性能的影響,藉助xtem 、 sims 、 srp 、 rbs 、 ie 、 raman 、 aes 、 xps等測試分析手段,我們發現,與傳統注氧隔離( simox )技術類似,存在著「劑量窗口」形成優質的soi材料,但在水等離子體離子注入方式中soi材料結構質量對劑量變化更為敏感,隨著注入劑量的增大, soi材料的埋層厚度增大而表層硅厚度減小。- acetolactate decarboxylase is purifed from cell extract by 50 % - 80 % ammonium sulfate - fractionation, 50, 2min heat treatment and deae - sepharose fast flow column chromatography, which we study the different ph and different buffer of deae - sepharose fast flow column chromatography and conclude ph 6
對其酶學性質進行了研究。 -乙酰乳酸脫羧酶經50 80硫酸銨分級沉澱、 50 , 2min熱處理、 deae - sepharosefastflow離子交換柱層析方法分離純化。- acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography
本文對來源於枯草芽孢桿菌( bacillussubtilis ) 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟,得到sds - paeg電泳純,通過n末端氨基酸序列分析驗證酶蛋白的純度。We purified the fusion mpt64 protein by the nickel - ion affinity chromatography
通過鎳離子親和層析獲得相對純的mpt64重組蛋白。Furthermore, the growth and the study of self - organized quantum dots structures become more and more important recently, and the application of self - organization technique become wider and wider in this thesis, we address the theory of film growth and the growth technique firstly more, the ways and characteristics of surface detection are prescribed we mainly report the growth process, results and discuss of self - organized quantum dots structures in the a12o3 substrates by s - k mode using ecr - mocvd, in the espd - u device the growth art of ain epilayer which is the preliminary foundation of self - organized gan / ain quantum dots structures, including the substrate cleaning, nitridation, the growth of buffer and the growth of gan and ain epilayer, is discussed we deliberately compare the test result of rheed xrd and afm and achieved the optimalized condition of ain at last we have successfully realized the growth of ain which is much smooth and better crystal quality moreover, we grow the self - organized gan / ain quantum dots structure in s - k mode because the limits of the heater temperature, we can not grow the atom - smooth epilayer of ain until now so we could not grow better quantum dots which have small diameter and big density but the self - organized quantum dots structures with better quality will be realized successfully if the substrate temperature is increased the thesis study belong to my tutor subject of national nature science foundation ( 69976008 )
本論文主要論述了在espd - u裝置上,採用電子迴旋共振等離子體增強mocvd ( ecr - pamocvd )方法,在藍寶石襯底上通過s - k模式自組裝生長gan aln量子點結構的生長工藝、結果及討論。而重點分析了自組裝生長量子點之前的aln外延層生長工藝,包括襯底清洗、氮化、緩沖層的生長和gan 、 aln外延層的生長;通過高能電子衍射、 x射線衍射和原子力顯微鏡測試,並且對這些測試結果進行了詳細的比較研究,得出了較優化的工藝條件,生長出了晶質較好、表面較平整的aln外延層;進而採用s - k模式自組裝生長了gan aln量子點結構。由於實驗裝置加熱爐溫度的限制,我們沒有能夠生長出原子級平滑的aln外延層表面,因而沒能夠生長出密度比較大和直徑比較小的量子點。Sige simox : oxygen ions with high dose were implanted into sige grown directly on silicon substrate for the first time, and sige - oi novel structure was formed successfully with additional high temperature annealing ; it has been confirmed that oxygen implantation with 45kev, 3 1017cm - 2 and annealing at 12500c in ar + 5 % o2 for 5 hours, are fit for the formation of sige - oi structure ; ge loss during the high temperature annealing has been observed, which is originated from ge volatility and ge diffusion ; it has been proposed to use nanoporous layer induced by h + / he + implantation to surppress ge diffusion and to use surface oxidation to overcome the upper limit of sige simox. sige smart - cut : hydrogen ions were implanted into sige material and followed by high temperature process ( 4000c to 7000c ) ; blistering study was done and suggested the possibility of sige layer transfer by smart - cut technology ; it is concluded that the bubble formation is easier in sige than in si, and the strain in sige / si and the difference of binding energy in sige and in si could possibly contribute to this effect. behavior of sige / si implanted with hydrogen : gave a detailed study on sige implanted by beamline or phi hydrogen implantation ; it has been found that great strain is introduced into sige by hydrogen implantation and this strain could be alleviated by high temperature annealing ; both for conditional beamline implantation and piii hydrogen implantation, 600 is appropriate for the post - implantation treatment
Sige - simox工藝方面:首次採用硅( 100 )襯底上直接外延的100nm厚sige的樣品中注入高劑量的o離子,通過退火處理成功制備了sige - oi新結構,即sige - simox工藝,證實了以45kev注入3 10 ~ ( 17 ) 7cm ~ ( - 2 )劑量的氧離子,隨后在氧化層的保護下經1250 , ar + 5 o _ 2氣氛的高溫退火( 5小時)過程,可以制備出sige - oi新型材料;實驗中觀察到退火過程中的ge損失現象,分析了其原因是ge揮發( ge通過表面氧化層以geo揮發性物質的形式進入退火氣氛)和ge擴散( ge穿過離子注入形成的氧化埋層而進入si襯底中) ,其中ge擴散是主要原因;根據實驗結果及實驗中出現的問題,對下一步工作提出兩個改進的方案:一是通過在si襯底中注入適量h ~ + / he ~ +形成納米孔層來阻斷ge擴散通路,二是可以通過控製表面氧化來調節安止額士淤丈撈要表面sige層中的ge組分,從而部分解決sigeThis paper studies the evolution of oil, gas and water in hinterland of junggar basin from point of view of hydrogeology. analyzes the mutual action among theta and the effect of formation water on the formation and distribution of oil - gas reservoirs, including applying some ions and characteristic factors of formation water to reflect the closed relations among the preservation conditions of the oil and gas, the closing degree of reservoirs and the oil - gas migration for the purpose of searching for favorable oil - gas accumulation zones by the varied features of formation water
從水文地質觀點和角度研究油氣水的演化,剖析水和油相互作用的關系,掌握地層水對油氣藏形成和分佈的作用,同時利用地層水的某些離子特徵和特徵系數來反映油氣的保存條件、地層的封閉程度、油氣的運移有著密切的關系,旨在利用水化學的變化特徵來尋找有利的油氣聚集帶。We have sifted 103 medicinal plants, roughly identified 17 plants might contain antifungal proteins. antifungal protein was purified from cassia sophera linn, by extraction, fraction with ( nh _ ( 4 ) ) _ ( 2 ) so _ ( 4 ), cation - exchange chromatography of cm - sepharose ff xk 26, the first cation - exchange chromatography of mono s and the second one, followed by gel filtration of superose 12hr
對茳芒決明進行了抗菌蛋白的分離純化:經粉碎、磷酸緩沖液浸提、硫酸銨沉澱、 cm - sepharoseffxk26陽離子交換層析、兩次monos陽離子交換層析、 superose12hr分子篩層析可得到具抗真菌活性的蛋白。2 nano ceramic material is infiltrated into plasma - spraying coatings by laser - cladding nano - material. the microstructures of the coating series gotten are studied in detail with x ray diffraction, scanning electron microscopy. it is concluded that after laser cladding the density of the nano material infiltrated coating is improved and superior to density of laser - remelting coating
( 2 )在等離子塗層的基礎上,利用激光熔覆將納米陶瓷材料熔滲到等離子塗層中,然後採用x射線衍射、掃描電鏡、能譜儀等,對陶瓷系列塗層的微觀組織與結構進行了詳細分析。分享友人