離胞的 的英文怎麼說
中文拼音 [líbāode]
離胞的
英文
cellifugal-
Aliquots of cells were mixed 0. 15 % mg / ml fb - 28, and kept at 4c for 30min, fusion assays were conducted : fluorescence was measured immediately at regular time - points with fluorescence spectrophotometer with an excitation wave length of 560nm and emission wave length of 590nm. the percentages of membrane fusion was calculated. by monitoring fusion using the r18 assay, we found that the fluorescent brightener 28 influenced membrane fusion of virus and midgut epithelia cells
此外,採用分子探針r18 (熒光標記物)標記病毒囊膜,體外分離中腸上皮細胞,將標記的病毒粒子與離體中腸上皮細胞混合后保溫,病毒吸附zh后,通過檢測熒光的變化來監測病毒粒子與上皮細胞的融合。Hypokalemia itself may produce a metabolic alkalosis, since an increase in excretion of hydrogen ions occurs when the concentration of potassium in the tubular cells in low
低鉀血癥可引起代謝性堿中毒,因為當在腎小管細胞中鉀離子濃度降低時氫離子的排泄就增加了。Autologous bone marrow stem cell transplantation in the treatment of bone nonunion
新生豚鼠神經幹細胞的分離培養及鑒定和標記Thus far we had confirmed that glia sense axon activity by taking in calcium
至此我們確認了神經膠細胞是以鈣離子流入細胞的方式感知軸突的活動。Adhesion to buccal epithelial cells of candida albicans isolates from oral lichen planus
口腔扁平苔蘚患者白色念珠菌分離株的頰細胞粘附力研究Other spiroplasmas have been isolated and cultured on cell-free media.
其它螺原體已被分離出來,並在無細胞的培養上培養。Dissolution and detachment of these cells create epithelial erosions that characterize the disease.
這些細胞的溶解和分離,產生惡性卡他熱特徵的上皮糜爛。In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分試驗中,首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液,然後經6層非連續percoll梯度離心法( 15 、 22 、 30 、 40 、 50和60 )分離,通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量,並以伊紅y排斥試驗測定細胞的存活率。Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1
我們以小鼠為模型,運用組織化學、免疫熒光組織(細胞)化學、流式細胞儀分選方法( facs )以及分子生物學手段,研究了小鼠乳腺的發育規律:小鼠乳腺組織中類乳腺幹細胞:小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定;小鼠類乳腺幹細胞分化的潛能;小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明: 1Oleifera was observed with lm. results showed that there were vegetative cell and generative cell in the pollen grain of genus camellia, the generative cell is more smaller than that of the vegetative cell. the generative cell was lay on the vegetative cell as an elongate spindle, and only the generative cell membrane and nuclear membrane which near the cell polar linked up the vegetative cell membrane, one of the linked area was located near by the aperture of pollen grain
結果表明,山茶屬植物花粉粒中含有生殖細胞和營養細胞,生殖細胞較小,呈長紡錘形,游離在營養細胞中,生殖細胞極軸兩端的細胞膜和核膜與營養細胞的細胞膜相連,其中一端的連結處位於花粉粒萌發孔附近。Isolation and long term culture of human primordial germ cells
人原始生殖細胞的分離和體外培養After fertilization granulosa cells and oocytes were isolated and continued to be cultured for 48 hours, and made into suspension ; the levels of fsh - r on the the granulose cells shall be immediately measured. results : 1
受精後分離顆粒細胞中文摘要和卵母細胞,將所剝離下的全部顆粒細胞繼續培養48 』 j 』時,製成混懸液,並及時進行顆粒細胞膜上fsh丑含量的測定。In the experiment, in case of the proliferative capability of eg cells, the composition of 0. 25 % trypsin + 0. 04edta was optimal. the growth behavior of icr murine eg cells from pgc was observed
傳代過程中,以0 . 25胰酶與0 . 04 edta聯合作用效果較好,對eg細胞的綜合損傷最小,離散傳代后eg細胞活力比較強, eg克隆出現最多,最高傳至6代。2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage
Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料,經傳代培養,獲得能連續而穩定傳至6代的,具有胚胎幹細胞諸多特性的eg細胞系。The life cycle of flowering plants may now be bridged at the level of free vegetative cells.
現在顯花植物的「生活史」可以在游離營養細胞的水平上連結起來。Sod2 from schizosaccharomyces pombe, which encodes a protein with homology to bacterial and mammalia na + / h + antiporters in plasma membrane and responses to efflux of na +, is an important gene i nvolving in homeostasis. though sod2 gene has been cloned, ectopic expression of sod2 in high plants has not been reported
Sod2在裂殖酵母( schizosaccharomycespombe )中編碼位於質膜上的na ~ + h ~ +逆向轉運蛋白,控制na ~ +外排,對于保持裂殖酵母細胞的離子均衡非常重要。In summary, we should investigate the more efficient cultural system for mammary orgnoids in vitro for enriched more mammary epithelial stem cells, and to screen the expression profiles in mammary epithelial stem cells to discover the specific marker for mammary epithelial stem cells in order to utilize the model of mammary epithelial stem cells to regenerate transgenic mammary glands in cow or sheep for human health, to uncover the mechanism of organogenesis, and differentiation
在此之前,仍然只能以造血幹細胞的特異性表達抗原scal - 1作為一個標記,進行類乳腺幹細胞的分析和分選研究。進一步研究類乳腺幹細胞的特異性表達譜,以期能夠找到類乳腺幹細胞的特異性表達抗原,分離得到類乳腺幹細胞,作為發育生物學和再生生物學研究的模型。Gene engineering based on reducing na + toxicity creats a new approach to plant salt tolerance. this is the first time expressing of sod2 in arabidopsis thaliana and maybe attribute to research the ion homeostasis in the cell. the sod2 gene was isolated from s. pombe by rt - pcr method and confirmed by sequencing
雖然sod2基因已經克隆,但是sod2在高等植物中異源表達及對其影響還未見報道,因此sod2基因在擬南芥中過量表達,對研究細胞的離子均衡及運用基因工程手段有效地提高植物的抗逆性具有重要意義。We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast
本文以單細胞的真核模式生物裂殖酵母( schizosaccharomycespombe )為研究材料,通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ ( 2 + )的分佈及相對濃度,以及不同周期時相細胞中gfp - cam的熒光強度變化,並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。The effort of manganese removal was studied and the kinetics of manganese removal was tried to establish. the factors of dissolved oxygen concentration, fe2 + concentration, ph, p concentration and closing of the filter were studied to evaluate their effort for biological manganese removal, and the correlation of residual manganese and oxidation - reduction potential was also discussed. as the iron content of water was high, experiment results showed that the reaction was zero order, as the iron content of water was low, the reaction was first order. the time needed for the cultivation of biological manganese removal was 60 70 days. the filter operated at the filtration rate of 8 10m / h, silica sand of effective size 0. 95 1. 25mm filled the filter to a depth of 1200mm
試驗結果表明,成熟后濾砂表面濾膜的x射線衍射圖譜與mno _ x ? 5h _ 2o ( x = 1 . 86 )的x射線衍射圖譜一樣,濾膜成熟后的結構在進水物質不發生變化的情況下不發生變化;合適的碳磷比對生物除錳有明顯的促進作用,試驗條件下的投磷量不會對出水造成二次污染;生物除錳需要亞鐵的參與,亞鐵的存在除了能夠促進微生物分泌胞外酶並刺激其活性外,還通過鐵離子的變價傳遞電子,催化錳離子的氧化反應,從而促進對二價錳的降解。分享友人