電免疫測定法 的英文怎麼說
中文拼音 [diànmiǎnyìcèdìngfǎ]
電免疫測定法
英文
electroimmunoassay- 電 : Ⅰ名詞1 (有電荷存在和電荷變化的現象) electricity 2 (電報) telegram; cable Ⅱ動詞1 (觸電) give...
- 免 : Ⅰ動詞1 (去掉;除掉) dismiss; relieve; remove 2 (避免) avoid; escape; avert 3 (免去) excuse s...
- 疫 : 名詞(瘟疫) epidemic disease; pestilence
- 測 : 動詞1. (測量) survey; fathom; measure 2. (測度; 推測) conjecture; infer
- 定 : Ⅰ形容詞1 (平靜; 穩定) calm; stable 2 (已經確定的; 不改變的) fixed; settled; established Ⅱ動詞...
- 法 : Ⅰ名詞1 (由國家制定或認可的行為規則的總稱) law 2 (方法; 方式) way; method; mode; means 3 (標...
- 測定 : determine; determination; setting-out; admeasurement; assignment; assay; finding
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Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated
解除光抑制后, ps活性恢復是一個復雜而有序的過程,需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理,加入林可黴素阻斷葉綠體蛋白質合成,利用尿素sds變性電泳分離類囊體膜蛋白,藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式,並進行定量分析。The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面,用於定向固定htk單克隆抗體,成功研製了檢測htk的壓電石英晶體傳感器,並基於標準scatchard繪圖法,計算出免疫反應的親和常數為1 . 85 106l / mol ,證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術,設計了的檢測htk的質量放大壓電石英晶體傳感器,該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測,應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定,實驗結果為癌癥的臨床診斷與治療提供了參考。In the present study, in order to investigate the effects of endogenous estrogen on the daergic terminals in amy and the daergic neurons in midbrain, fast cyclic voltammetry ( fcv ) was used to examine da release evoked by electrical stimulation from amy of female rats in different phases of estrus cycle, ovx rats and male rats. and tyrosine hydroxylase ( th ) immunohistochemi stry was employed to measure the numbers of daergic neurons of ventral tegmental area ( vta ) and substantia nigra ( snc ) in the rats
因此,為進一步探討大鼠內源性雌激素水平的變化對amyda能神經系統及中腦da能神經元的影響,本工作應用快速周期伏安法( fcv )在體監測了處于動情周期各期雌鼠、 ovx鼠和雄鼠經電刺激誘發的amyda釋放,並應用酪氨酸羥化酶( th )免疫組化方法測定了以上各組大鼠腹側背蓋區( vta ) 、黑質( snc )的th陽性神經元數目。So piezoelectric immunnosensors is gradually applied to some fields such as biochemical research, clinical analyze, environment and industry process assay. according to different method of immobilized antigens or antibodies, developed some new piezoelectric immunnosensors to determine proteins in consult serum
湖南大學分析化學碩士論文李丹200年6月本文結合壓電免疫傳感器抗體(抗原)固定化方法的不同,研製了一些新的壓電免疫傳感器用於參考血清中的一些蛋白的測定。In this study we had used the sensor to detect antibody - antigen reactions of fasciolsis, nd, ibd and eds. it is proved the sensor is fitted to use in detecting antibody - antigen reactions of these diseases. the results of detecting antibody - antigen reactions of fasciola gegatica, nd, ibd and eds by using the sensor basically conform to that with standard samples
在實驗研究中,我們先後應用電化學免疫傳感器測試了大片形吸蟲,雞新城疫( nd ) 、雞傳染性法氏囊病( ibd ) 、雞減蛋綜合征( eds )等的抗原抗體反應,結果與我們先前初步設定的判斷標準基本相符。At present, the main methods used in the study on chinese traditional medicine in gastrointestinal dynamics animal experiment are gastric emptying and small intestine motional rate mensuration, parent electric bridge strain gauge mensuration, gut hormone determination ( gas, mot, ss et ), immunohistochemical method, excised gastrointestinal smooth muscle dynamoscopy, intragastric and intestine pressure tracing
摘要中藥在胃腸動力動物基礎實驗研究,目前主要採用胃排空小腸推進率測定法、胃腸道腔外電橋式應力傳感器記錄法、胃腸激素測定(胃泌素、胃動素、生長抑素等) 、免疫組織化學方法、離體體胃腸平滑肌肌條動力測定、胃腸內壓描記法。Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法:採用成年大鼠肺組織無血清培養,運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿( pc )合成量; rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶( cct ) mrna含量;透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化;免疫組織化學染色檢測glu的受體nmdar1亞單位的表達;生化測定肺組織乳酸脫氫酶( ldh )釋放量和肺組織勻漿中一氧化氮合酶( nos )活性、一氧化氮( no )生成量、超氧化物歧化酶( sod )水平以及丙二醛( mda )含量。This paper describes techniques for detecting early markers of dn, including routine methods, such as radioimmunoassay, chemical luminescence immunoassay, enzyme - linked immunosorbent assay etc., and biosensors and microelectrochemical biosensors, such as piezoid sensor, fluorescence biosensor, surface plasmon resonance biosensor etc
本文介紹糖尿病腎病早期標志物的檢測技術,包括放射免疫測定法、化學發光免疫測定法、酶聯免疫吸附測定法等實驗室常規檢測方法,以及石英晶體免疫傳感器、熒光生物傳感器、表面等離子體共振生物傳感器和微型電化學生物傳感器等檢測技術。In this paper, we developed three novel immunosensors and a mediator - free enzyme sensor, based on efficient immobilization of biomolecule and signal - amplified methods to improve detection sensitivity and decrease the detection limit. the detailed materials are shown as follows : ( 1 ) an amplified immunosensor with highly sensitivity has been proposed based on precipitation of an insoluble product on functionalized electrode ( in chapter 2 ). anti - higg was immobilized onto the surface of gold electrode modified with 1, 6 - hexanedithiol and colloidal au interface
本文結合有效的生物活性組分的固定方法,採用信號放大技術提高分析信號、降低檢測下限,發展了三種新型的免疫生物傳感器以及一種無電子媒介體的酶生物傳感器,主要內容如下: ( 1 )提出了一種新型的基於酶催化沉積質量放大的高靈敏壓電免疫傳感器(第2章) 。分享友人