電泳成分 的英文怎麼說

中文拼音 [diànyǒngchéngfēn]
電泳成分 英文
electrophoretic fractians
  • 電 : Ⅰ名詞1 （有電荷存在和電荷變化的現象） electricity 2 （電報） telegram; cable Ⅱ動詞1 （觸電） give...
  • 泳 : 動詞（游泳） swim
  • 成 : Ⅰ動詞1 （完成; 成功） accomplish; succeed 2 （成為; 變為） become; turn into 3 （成全） help comp...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  1. Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis

    首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。
  2. That is the premise of the bg / ha electrophoresis codeposition. the laws of the electrophoresis deposition of the bg and ha partic les were found by the study on each of their deposition under the different conditions. the electrophoresis codeposition of the bg and ha particles had been studied and the bg / ha graded coating, which is compact in the bottom layer and porous near the surface layer, had been prepared on the surface of the dental implant after the low temperature heat treatment ( about 740 ) and fast firing ( 50 - 80 / min, heat preservation time was 5 - 8min. )

    以bg微粉和ha微粉作為塗層原料，通過研究bg和ha微粉在非水介質中的分散情況和帶電特性，選擇冰醋酸為介質，使分散在其中的bg顆粒和ha顆粒表面均帶上正電荷，為電泳共沉積提供前提條件；通過對不同條件下bg 、 ha各自電泳沉積的研究，探索出了兩者電泳沉積的規律；通過對bg和ha在冰醋酸中電泳共沉積以及后續低溫（ 740左右）快燒（ 50 ? 80 min ，保溫5 ? 8min ）熱處理的研究，在鈦合金牙根種植體基體上成功制備出了底層緻密而表層多孔的bg ha梯度塗層。
  3. To prove up ulteriorly the components of ants, the author has separated and purified the polypeptides of formica rufa linnaeus and studied their biologic and officinal activities. at the same time, the author has mensurated the molecular weight of polypeptides by sds polyacrylamide electrophoresis to analyze the polypeptides quantific - ationally. in order to provide the scientific basis for studying and empoldering the ants, we have done these studies

    為進一步探明螞蟻體內的活性成分以開發和利用其藥用價值，本文對紅褐林蟻的提取物中的多肽進行了分離、純化並對其生物活性和藥用活性進行了研究，通過電泳測定了多肽的分子量，從而為進一步研究開發螞蟻提供科學的依據。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. Determination of ofloxacin in ofloxacin tablet and intramuscular injection by capillary zone electrophoresis

    氧氟沙星片劑與針劑中有效成分的毛細管區帶電泳快速測定
  6. Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated

    解除光抑制后， ps活性恢復是一個復雜而有序的過程，需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理，加入林可黴素阻斷葉綠體蛋白質合成，利用尿素sds變性電泳分離類囊體膜蛋白，藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式，並進行定量分析。
  7. This project which is based on the demand of increasing the electron tube ’ s qualities totally and reducing the manufacture cost has done a large amount of investigative work as follows to improve and perfect the technologies for the important part of electron tube manufacture ? the grid surface processing : on the surface processing of the molybdenum grid, the primary purpose is to reduce thermionic emission and secondary electronic emission of the grid. by the constantly experiment and grabbling the different technology routes, we have successfully developed these new technologies on the tac and zrc electrophoresis and electroplating platinum black of the grid, and made its surface cladding quality very stable and reliable

    本課題是基於整體提高電子管的質量和降低生產成本的要求，對電子管生產中的重要部分? ?柵極的表面處理技術進行改進和完善，主要在以下方面進行了深入研究:在鉬柵極表面處理方面，主要為實現降低柵極的熱電子發射和二次電子發射，通過不同工藝路線的不斷試驗和摸索，成功開發出柵極電泳tac 、 zrc和電鍍鉑黑的新工藝，使柵極的塗覆質量穩定可靠。
  8. Relative writing age analysis of the black roller pen inks by capillary zone electrophoresis

    區帶毛細管電泳法分析黑色簽字筆墨水字跡相對形成時間
  9. During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively

    提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄，剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。
  10. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  11. Then were electrophored. the extent of dna migration were measured. index - percentage of " comet " cell and " comet tail " were analysed that indicated when vero cells were treated with 2 u g / ml quinocetone vero cells got midium - grade damage, and were treated with 6 u g / ml olaquindox vero cells got midium - grade damage

    喹乙醇染毒劑量在2 10 g ml時細胞存活率90 ，以2 10 g ml劑量的喹乙醇染毒處于對數生長期的vero細胞，后經過電泳，通過分析彗星樣細胞發生率和彗尾長短等指標，結果表明染毒劑量在6 g ml對dna造成中度損傷。
  12. Index - percentage of " comet " cell and " comet tail " were analysed that indicated the optimum time is 2 ~ 3h. the degree of dna damage by quinocetone and olaquindox were detected by scge assay, when vero cells were treated with 1 ~ 5 ug / ml quinocetone the percentage of live cells was above 85 % and with 2 - 10ug / ml olaquindox the percentage of live cells was above 90 %

    喹烯酮染毒劑量在1 5 g ml時細胞存活率85 ，以1 5 g ml劑量的喹烯酮染毒處于對數生長期的vero細胞，后經過電泳，通過分析彗星樣細胞發生率和彗尾長短等指標，結果表明染毒劑量在2 g ml對dna造成中度損傷。
  13. It was found that b - 6 isolated by the method of distinct zone of clearing in cellulose - congo red agar medium combined with measuring the enzyme activity of liquid culture filtrates had comparatively higher cellulase activity. by measuring activity of cellulase of strains growing in medium with different carbon sources and of washed mycelium induced by different carbon sources in certain time, it found that the formation of cellulase was regulated by the nature of the carbon source used for b - 6 and as3. 3711

    真菌纖維素酶是一種誘導酶，碳源同時也是主要的誘導物來源，為了研究碳源對真菌纖維素酶合成的誘導機理，本文利用液體生長培養和洗滌菌絲誘導培養法研究了不同碳源對兩菌株的誘導特性，並用電泳分析法研究了不同碳源的誘導酶譜。
  14. Isozymes in different tissues of three stains of crucian carp were studied comparatively. the results indicated that pengze crucian carp is different from silver crucian carp in biochemical level, they probably came from different region. the electrophoretogram of pengze crucian carp and silver crucian carp all included the basal patterns of wild crucian carp

    通過對三種鯽魚品系不同組織同工酶的電泳圖譜的比較研究，揭示了彭澤鯽和銀鯽至少在生化水平上已有明顯的分化，很可能起源於不同的地區，獨立演化而形成。
  15. . moreover, some samples appeared several active bands in the gel, which indicated the existence of different types of sod or multi - subunits of sod in these samples. the bacterial strain 276 is a gram - negative rod bacterium and there are more than 3 polar flagella, which observed after the gram ' s staining and flagellum staining

    同時，利用非變性聚丙烯酰胺凝膠（ page ）電泳后的凝膠顯色反應，發現一些樣品出現了多條活性帶，這可能是因為在這些細菌提取物樣品中含有不同類型的sod分子，或是同一類型的sod含有多個亞基組成。
  16. A page gel, stained by the petiodic acid - schiff ' s method to reveal glycoproteins, further displayed that the bindng - protein was a glycoprotein belonging to lectin, which contained 17. 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method. peptide mapping was comparable to the reported protein in protein bank. the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase, aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b, glutamyl - trna reductase, histidyl - trna synthetase

    連續梯度聚丙烯耽胺凝膠電泳、 sds一聚丙烯酞胺凝膠電泳和等電聚焦的結果表明該蛋白分子量為1 「 . skda ，由二個相同的亞基組成，亞基分子量為」 . ikda ，等電點為8 . 25 .糖蛋白染色結合考馬斯亮藍染色的結果證實此結合蛋白是個糖蛋白，其中糖含量為17 . 44 % ，蛋白含量為82 . 56 % .凝集反應確定該糖蛋白也是一個凝集素
  17. The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates, and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant

    沉澱和上清液的不連續十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（ sds - page ）和高效凝膠排阻層析分析結果表明，還原脲變性蛋白溶菌酶在稀釋復性過程中除了能夠復性成天然態蛋白溶菌酶分子外，還會形成可溶的蛋白溶菌酶分子二聚體和三聚體，二聚體和三聚體主要是靠分子間二硫鍵的錯配連接而成的；可溶的蛋白溶菌酶分子二聚體之間通過非共價鍵相互作用而形成集聚體沉澱，而可溶的三聚體溶菌酶分子則仍處于復性液上清液中。
  18. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  19. The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot

    4 .電泳遷移率改變實驗有明顯的滯后帶形成，表明lps刺激后，肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合，參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。
  20. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
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