電泳條帶 的英文怎麼說

中文拼音 [diànyǒngtiáodài]
電泳條帶 英文
electrophoretic band
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  1. That is the premise of the bg / ha electrophoresis codeposition. the laws of the electrophoresis deposition of the bg and ha partic les were found by the study on each of their deposition under the different conditions. the electrophoresis codeposition of the bg and ha particles had been studied and the bg / ha graded coating, which is compact in the bottom layer and porous near the surface layer, had been prepared on the surface of the dental implant after the low temperature heat treatment ( about 740 ) and fast firing ( 50 - 80 / min, heat preservation time was 5 - 8min. )

    以bg微粉和ha微粉作為塗層原料,通過研究bg和ha微粉在非水介質中的分散情況和特性,選擇冰醋酸為介質,使分散在其中的bg顆粒和ha顆粒表面均上正荷,為共沉積提供前提件;通過對不同件下bg 、 ha各自沉積的研究,探索出了兩者沉積的規律;通過對bg和ha在冰醋酸中共沉積以及后續低溫( 740左右)快燒( 50 ? 80 min ,保溫5 ? 8min )熱處理的研究,在鈦合金牙根種植體基體上成功制備出了底層緻密而表層多孔的bg ha梯度塗層。
  2. 4 hemolymph immune reaction of p. americanna after nature infection with m. anisopliae dripping the cockroach body with m. anisopliae showed that the m. anisopliae spores can invade into the hemolymph coelom of p. americanna through cockroach cuticle and produce blastospores

    美洲大娩對金龜子綠僵菌cqmal02菌株的免疫反應經sds page發現,注射誘導后的蜚蛾血淋巴在43kda處出現了一新的蛋白,但含量很低。
  3. The supernatants were dialyzed against 50 mm phosphate buffer ( pb ) ( ph 6. 5 ), and passed through a blue - sepharose 6b column ( 3x30 cm )

    Iptg誘導表達目的蛋白,上清液經sds一page后看到約32kd的目的
  4. The substance with antibacteria action obtained from forest frog is made up of alanine, aminoacetic acid, leucine, isoleucine, proline, aminoglutaric acid, threonine, serine, lysine. the substance with antibacteria action is a kind of poly peptide with a micromolecul

    純化的林蛙皮膚抗菌活性物質經尿素? sds ? page分析,表現為一,分子量約為6 . 28kda 。
  5. Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively

    本實驗用sds - page和免疫印跡的方法,用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定,結果表明:抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應,同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白存在,表明在植物中有類三磷酸肌醇受體蛋白的存在;用免疫膠體金方法,發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。
  6. The purity was detected by sds - page, and only igg ' s heavy chain and light chain protein tope were showed up. the activity of igg was tested by technique of immune credeschs gold

    用sds - page進行igg的純度檢驗,結果出現兩蛋白,是igg的重鏈和輕鏈,說明純化的程度較高。
  7. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵,用緩沖液抽提后,經硫酸按沉澱, deae一纖維素離子交換層析,聚丙烯酞胺凝膠洗脫等純化步驟獲得的植酸酶,用sds一page檢測為一均一譜,其分子量約為78kd 。
  8. . moreover, some samples appeared several active bands in the gel, which indicated the existence of different types of sod or multi - subunits of sod in these samples. the bacterial strain 276 is a gram - negative rod bacterium and there are more than 3 polar flagella, which observed after the gram ' s staining and flagellum staining

    同時,利用非變性聚丙烯酰胺凝膠( page )后的凝膠顯色反應,發現一些樣品出現了多活性,這可能是因為在這些細菌提取物樣品中含有不同類型的sod分子,或是同一類型的sod含有多個亞基組成。
  9. The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )

    經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page分析,獲得一約120kda的表達; iptg誘導表達后提取原生質膜測定透明質酸分解酶活力,表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。
  10. Materials and methods in our study, first the e. coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis. the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i. the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis

    同預想結果基本吻合,大規模培養后純化得到的融合蛋白通過sds page顯示在52kda處有一,而酶切后產物結果顯示在26kda處分另有兩蛋白,其中一為gst ,另一為hbrp ,基本符合實驗結果; hbrp對tpk活性的抑制呈劑量依賴性。
  11. The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male

    Page結果表明:麗蠅蛹集金小蜂明顯存在2雌特異性-卵黃蛋白,分子量分別為181kd和136kd ; sds - page分析:存在3雌特異性,其分子量為123kd 、 120kd和91kd ,由此,可推定卵黃原蛋白( vitellogenin , vg )和卵黃磷蛋白( vitellin , vn )由2個蛋白組成,其中分子量較大的蛋白由2個亞基組成;雙向結果顯示,在120kd附近有兩個特異性點,其等點為5 . 5和5 . 7 ;雙擴散表明,麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應,而與雄蜂血淋巴無免疫反應,說明了vg與vn具有免疫同源性,是雌特異性蛋白,且由脂肪體合成。
  12. Apoptotic peak. agarose gel electrophoresis showed that 185 - base pair ladder characteristic of the dna degradation that occurs in apoptotic cells induced by a

    出現階梯狀流式細胞儀檢測出現典型的凋亡峰,提示
  13. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母, g418篩選出多拷貝插入的單克隆,甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白,培養液上清經sds - page分析,在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ,能與羊抗hcmv多克隆抗體發生發應的
  14. Mineralization of soil organic carbon and its motivating factors to the dragon spruce forest and alpine meadows of the qilian mountains

    圖譜共分離出34不同遷移率的譜;依據蛋白圖譜等
  15. The inhibition of lower concentrition of tps and egcg is stronger than the inhibition of higher concentrion for cancer cells

    處理后的d6細胞, dna瓊脂糖凝膠出現典型的dna梯狀
  16. ( 2 ) effects on mouse spleen of so2 challenge : we found significant apoptotic changes of mouse spleen through tem observation and dna electrophoresis analysis and flow cytometric analysis. we found condensed, marginating, half - moon like apoptotic lymphocytes both in white pulp and red pulp ; we found significant dna degradation with dna ladders from the dna electrophoresis analysis in the 168 mg / m3 so2 treated group ; we also found marked increase of apoptotic rate between 168 mg / m3 so2 treated group and control group from the flow cytometric analysis

    ( 2 )二氧化硫吸入可引起小鼠脾臟細胞出現明顯的凋亡改變,紅髓區和白髓區淋巴細胞出現核固縮,染色質凝聚、邊集; dna凝膠分析發現168mg m ~ 3二氧化硫染毒組出現典型的dna梯形;流式細胞分析也發現高劑量染毒組的小鼠脾細胞凋亡率增加,並且與對照組相比有顯著性差異, p 0 . 05 。
  17. The pcr products of gyra of a resistant strain ( sll - 3 ) was sequenced and analysed. when compared to the corresponding sequence of the gyra of salmonella typhimurium from the nucleotide sequences data of the gyra reported by griggs, 5 mutants sites were found in the qrdr. dma sequencing identified in the strain sll - 3 ser to phe change at codon 83 of gyra gene

    對所試菌株染色體的pcr產物行hinfi酶切后檢測表明, cip高敏菌株( 6株)得到預期大小為363bp 、 206bp 、 99bp的3個;而耐藥菌株和中敏菌株( 10株)檢測到大小為363bp和305bp的2個
  18. I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

    從橡膠樹pr107品系的嫩葉提取基因組dna ,用限制性內切酶ecor 、 hinofll分別酶切,瓊脂糖凝膠分離並轉膜后,用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析,結果表明, ecor酶切在約3 okb處有一雜交出現, hi 。
  19. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因,克隆到桿狀病毒表達載體pfastbacl中,構建成重組轉座載體pfb1 - cee ,轉化dh10bac大腸桿菌感受態細胞,篩選陽性菌落,抽提大分子質粒dna ,獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ,脂質體介導轉染sf9昆蟲細胞,出現細胞病變后,收集含有重組桿狀病毒顆粒的培養上消,重新感染sf9細胞,收集sf9細胞,進行12 . 5 sds -聚丙烯酰胺凝膠,可見表達的蛋白
  20. Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected

    Rapd (隨機擴增多態性dna )技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術,它建立於聚合酶鏈式反應( pcr )技術基礎之上,利用隨機合成的寡聚核苷酸序列為引物(一般為10個bp ) ,分別與dna的兩單鏈結合,在dna聚合酶的作用下,對基因組的特定區域進行pcr擴增,其結果為不同大小和數目的dna譜即rapd圖譜,可反映基因組相應區域的dna多態性。
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