電泳緩沖液 的英文怎麼說
中文拼音 [diànyǒnghuǎnchōngyè]
電泳緩沖液
英文
electrophoresis buffer-
In this sense, ida plays dual effects, tridentate chelating to cu ( 2 ) and bridge between two cu ( 1 ) andcu ( 2 ). 3 metal complexes were selected as the appropriate for the study of cleavage plasmid pbr322dna by gel electrophoresis technique. the results showed ni and mn complexes could cleave effect - ively dna in the presence of h2o2 at physiological ph and temperature, whereas individual zn complex could cleave effectively dna
通過電泳實驗研究了一系列金屬配合物與pbr322dna的作用，發現在tris - hcl緩沖溶液中，生理條件下，鎳、錳配合物在共反應物h _ 2o _ 2存在下能夠很好的斷裂dna ，而zn配合物單獨作用就能夠使dna由ccc型轉化為oc和linear型。Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。In this study, the suitable parameters for the introduction of plasmids phz1358 and pset152 into s. nanchangensis ns3226 from e. coli were tested for developing an conjugation system for s. nanchangensis ns3226. a dnd gene cluster, which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e. coli into s. nanchangensis ns3226
將克隆在整合型載體pset152上的變鉛青鏈黴菌1326的dnd基因簇通過接合轉移導入野生型南昌鏈黴菌ns3226中進行異源表達，觀察到接合轉移子的dna獲得了在含fe ~ （ 2 + ）的電泳緩沖液中電泳時降解的表型。Method to collect respectively 180 unrelated males " venous blood 500ul, who lived in shanxi province, 120 unrelated mongolians " venous blood 500ul, who lived in the inner mongolia autonomous region, and the blood is anticoagulant with edta, then to extract dna by using the method of phenol - chloroform after ingested by proteinase k at 56 and amplify the dys413 site by using pcr
方法採集180例山西漢族和120例內蒙古蒙古族男性無關個體靜脈血各500ul ， edta抗凝，用tkml液反復洗滌至無色，加入2蛋白酶k緩沖液180ul ，蛋白酶k （ 20mg ml ） 20ul ，在56消化至液體清亮為止，用酚-氯仿法抽提dna ， pcr擴增dys413位點， 6非變性聚丙烯酰胺凝膠電泳， 1硝酸銀染色分型。Effects of separation voltage and composition of running buffer on the plateau were investigated and the optimized separation condition was determined
通過考察施加的電壓和運行緩沖液的組成對氯氮平電泳峰平臺形成的影響確定了最優分離條件。分享友人