電泳轉化 的英文怎麼說

中文拼音 [diànyǒngzhuǎnhuà]
電泳轉化 英文
electrophoretic variants
  • 電 : Ⅰ名詞1 （有電荷存在和電荷變化的現象） electricity 2 （電報） telegram; cable Ⅱ動詞1 （觸電） give...
  • 泳 : 動詞（游泳） swim
  • 轉 : 轉構詞成分。
  • 轉化 : 1. （轉變） change; transform 2. [化學] inversion; conversion
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

    Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。
  3. The e. coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4. 4. the transformation was induced with iptg, then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein, and the fusion protein is about 90kd

    4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌，提取初提物中的總蛋白，進行sds一聚丙烯酞胺凝膠電泳，檢測表達的融合蛋白大小越為gokd 。
  4. In this sense, ida plays dual effects, tridentate chelating to cu ( 2 ) and bridge between two cu ( 1 ) andcu ( 2 ). 3 metal complexes were selected as the appropriate for the study of cleavage plasmid pbr322dna by gel electrophoresis technique. the results showed ni and mn complexes could cleave effect - ively dna in the presence of h2o2 at physiological ph and temperature, whereas individual zn complex could cleave effectively dna

    通過電泳實驗研究了一系列金屬配合物與pbr322dna的作用，發現在tris - hcl緩沖溶液中，生理條件下，鎳、錳配合物在共反應物h _ 2o _ 2存在下能夠很好的斷裂dna ，而zn配合物單獨作用就能夠使dna由ccc型轉化為oc和linear型。
  5. After the protein was electrophoresised and purified, the protein activity was detected by elisa, the protein activity of vp1 is higher than vp0 vp3. at last, the activity of vp1 made in our lab was detected with the agentia made in our lab

    將陽性重組子轉化到大腸桿菌er2566細菌內，用ipig進行誘導表達蛋白，蛋白經電泳、純化，然後用elisa方法檢測蛋白活性， vp1蛋白活性相對高於vp0 、 vp3 。
  6. The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli

    從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e
  7. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  8. The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )

    經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析，獲得一條約120kda的表達條帶； iptg誘導表達后提取原生質膜測定透明質酸分解酶活力，表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。
  9. Cells were then harvested by centrifugation and the pellet was resuspended in phosphate - buffered saline ( pbs ) containing 5 mm edta. after sonication, debris was removed by centrifugation at 10000 x g for 15 min at 4

    挑取轉化后的大腸桿菌提取質粒， ecori和hindln酶切質粒進行鑒定，瓊脂搪電泳顯示含有大約800kb的目的片段。
  10. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  11. The recombinant plasmid was translated into e. coli dh5 and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 22ku protein which was equal to chicken ifn - y protein in molecular weight was expressed in e. coli dh5

    將重組質粒轉化大腸桿菌dh5 ，於37誘導培養8h ， sds - page凝膠電泳表明該基因在大腸桿菌中獲得了高水平表達，表達的雞ifn -融合蛋白分子量約為22ku 。
  12. Isolation and purification of modified yacs from yeast strain have been carried out by pulsed - field gel electrophoresis. the modified yacs are expected to generate plant artificial chromosome after being transferred into arabidopsis protoplast by liposome - mediated method

    用脈沖電泳將經過同源重組的修飾的yac克隆分離出來通過脂質體轉化植物的原生質體，希望得到在植物細胞中穩定存在的植物人工染色體。
  13. Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained

    Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。
  14. Gene had been highly expressed after incubating the transformed strains in the inducing culture

    基因已被轉化，誘導培養后的蛋白電泳顯示
  15. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  16. Methods : extracting the total rna of human pbmc, the objective include 60 healthy blood donator, 30 patient with viral encephalitis and multiple sclerosis and parkinsonian syndrome, 30 patient with schizophrenia and affective disorder, this indviuals were inpatients or outpatients of the first hospital of chongqing university of medical science from december, 2000 to june, 2001. using nested rt - pcr techique to detect borna disease virus " middle fragment in orf i, and using southern blot hybridization to analyze the pcr product

    重慶醫科大學碩士學位論文方法：提取從2000年12月至2001年6月在重慶醫科大學第一附屬醫院神經科和精神科住院及門診的60例健康獻血者、 30例包括原因未明的病毒性腦炎、多發性硬化、帕金森綜合征，以及30例包括精神分裂癥和情感障礙患者pbmc中的總kn 』 a ，採用套式逆轉錄聚合酶鏈反應estedrticr ）技術進行了bdvorf基因中部片段的檢測，並對pcr產物進行電泳分析及southernblot雜交分析。
  17. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  18. And another change was that the spore of the transformant was difficult to release, p3a was not useful in genetically engineered strain construction in this way

    此外， pcr擴增發現轉化子比出發菌ybt - 1520多了cry1ab的特異帶， sds - page電泳證實cry1ab的存在。
  19. The e2 gene was amplified by rt - pcr, then examined the fragment by electrophoresis. after purification and insertion into pucm - t vecter, the recombinant plasmid pbne2pi and pbne2pii were obtained. then they were transfected e. coli jm109 and screened positive clones by blue or white plaques. the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis

    通過rt - pcr擴增e _ 2基因，電泳測定擴增片段的大小，經純化后，連接于pucm - t載體，獲得重組質粒pbne2p 、 pbne2p ，轉化e ? colijm109 ，經藍白斑篩選，挑取陽性克隆，提取質粒，直接電泳鑒定和酶切鑒定。
  20. Aralia elate ( miq. ) seem is a kind of uncultivated vegetable from the mountain changbai. the process of somatic embryogenesis includes morphogenesis stage of calli, conversion stage of embryonic calli, embryogenetic stage and regeneration stage of plant in aralia elate (

    本論文採用sds -聚丙烯酰胺凝膠電泳技術，對龍芽?木體細胞胚發生過程中愈傷組織形態建成、胚性愈傷轉化、胚狀體發育和植株再生四個階段的可溶性蛋白的動態變化進行分析。
分享友人
分享到 Line

分享到臉書