預孵化 的英文怎麼說
中文拼音 [yùfūhuà]
預孵化
英文
preincubation-
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質Group division : experiments were performed in 3 different conditions, i. e., normal 4 medium, high k * stimulated medium, and ca2 + - free medium. hippocampal slices from a same rat were then divided into 3 treatment groups : control group ? lices were oxygenated with 95 % o2 / 5 % cc > 2 and incubated at 35 ~ 36 ? for 30min ; hypoxia group ? lices were oxygenated and incubated on the same conditions as the control group, then the mixture gases change into 95 % o2 / 5 % co2 - hypoxic condition were remained for lomin ; ginkgolide b preincubation group ? lices will be preincubated at the normal oxygenated medium containing loomm ginkgolide b, 10 min later, repeated hypoxic treatment
3實驗採用隨機區組設計，動物隨機分為三組，各組動物制備的海馬腦片分別進行三種不同介質的灌流實驗，即正常介質組、高k ~ +去極化組和無ca ~ （ 2 + ）組；各組中由同一隻動物製成的海馬腦片再隨機分到三個不同的處理組，即：正常對照組、低氧組和ginkgolideb預孵育組。This session will explore incubator service offerings with revenue - generating potential, such as having affiliate clients, providing pre - incubation services, hosting venture conferences and partnering with local universities to offer educational courses
在本階段，將解釋所能獲得潛在收益的孵化服務：如：開辦分公司，提供預前孵化，風險評估會議，及當地的大學提供教育培訓。
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