預平衡柱 的英文怎麼說
中文拼音 [yùpínghéngzhù]
預平衡柱
英文
pre-equilibration column-
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質When injecting samples, chromatographic column should be completely flushed and balanced with mobile phase ; if the system applicability is not up to regulation, or if filling agents are damaged, replace with new similar chromatographic column for analysis ; if operation according to procedures can not reach scheduled separation due to existence of certain difference between degree of bond of chemically bonded - phase of similar filling agents and performance, another brand of similar chromatographic column can be used for test
3進樣前，色譜柱應用流動相充分沖洗平衡，如系統適用性並不符合規定，或填充劑已損壞，則應更換新的同類色譜柱進行分析，由於同類填充劑的化學鍵合相的鍵合度及性能等存在一定差異，往往依法操作達不到預定的分離時，可更換另一牌號的同類色譜柱進行試驗。Without ammonium sulphate fractionation and dialysis, the supernatant of crude extract was directly loaded on deae - sepharose cl 6b column equilibrated by phosphate buffer ( 50mmol / l, ph7. 8 ), and the enzyme fraction was not absorbed on the column but impurities were absorbed
粗酶液無需硫酸銨沉澱及透析，即可引入磷酸緩沖液（ 50mmol l ， ph7 . 8 ）預平衡的deae - sepharosecl6b柱，上柱後用平衡緩沖液洗至基線穩定。 afpga不被吸附而直接流出。
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