高免抗體 的英文怎麼說
中文拼音 [gāomiǎnkàngtǐ]
高免抗體
英文
hyperimmune antibody-
Heamagglutination tests were applied to detect virus in allantoic fluid of chicken embryos which were infected by b95 gathered from the vaccinated chickens " cloacal and oral cavity. the results show that the virus may be detected from 2 days to 11 days after the chicken being vaccinated. the hi antibodies were measured by heamagglutination inhibition tests. there is no significant difference between the immunized and the control chickens which were fed in one case. chickens were immunized with b95 by different immunization meathods or with different vaccines by the same meathod. lt is demonstrated that eyedrop, drinking water, spray or muscle injection all can stimulate good effects, but eyedrop and spray seem to be the best meathods. b95 immunized chicken have relatively higher hi titers and it also can last for a longer time than others
但如果兩者相隔10天以上免疫， b95免疫不受h120的影響；如果同時免疫b95和h120 ，加大b95的免疫劑量也能獲得良好的免疫效果。用棉拭子采b95免疫雞口腔、泄殖腔的分泌液，檢測其中病毒的存在，結果免疫后2 11天雞口腔和泄殖腔中均有病毒的存在，說明b95免疫雞帶毒時間長。研究結果表明， b95具有不受母源抗體干擾、 hi抗體產生快、水平高、持續時間長、同居擴散性強等特點，因此b95是一株優良的、具開發前景的新的新城疫疫苗株。In recent years, the outbreak of nd appear some new characters, it is reported that the amount of immunity defeat on nd increased, even chicks with high hi titer can be infected by ndv virulent strain. the gene - engineering vaccine is badly needed to control nd outbreak. so newcastle disease virus strain b95, a naturally avirulent and heat stable strain, was introduced from australia
新城疫是國際獸疫局公布的16種烈性傳染病之一。近年來，新城疫的爆發出現了許多新特點，免疫失敗報道增多，甚至抗體水平較高的雞群也發病。為控制新城疫的爆發，急需研製基因工程苗。From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞，從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞，並且這種凝集可以被特異性抗血清所抑制。The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label
酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用，其靈敏度取決于標記酶的高度純化和高轉化率。One group was immunized with cdv vaccine, and the other group was cav. serum samples and eggs were collected weekly for detected antibody titers. high titer egg yolk was collected for following igy extraction
分別用cdv 、 cav的疫苗免疫產蛋雞，每周檢測雞血清和卵黃中的抗體水平，收集抗體水平高的雞蛋用於提取純化卵黃抗體igy 。However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting
利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。Results the conjugation rate, high molecular conjugate content of and the antibody titer induced with pneumococcus type 1 conjugate vaccine prepared by cyanogen bromide activation were significantly higher than that by amine reduction
結果採用溴化氰活化法制備的結合物，較胺還原法具有較高的結合率及高分子結合物含量，免疫小鼠產生的抗體效價較胺還原法明顯提高。The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。Spf chickens with 21 - day - old were infected subcutaneously with oil - emulsion vaccine of ibdv of germinal or cellcular and injected intramuscularly with different dosages bursin which gain through ultrafilter. lt is proved that bursin of chickens and ducks can both shorten the time of antibody induced against ibdv, raise the level of serum antibody. they make chickens obtaining strong immunocompetente in a short time. agp liters of the group of infecting 0. 4mlcbs + ibdv of germinal and 0. 8mlcbs + ibdv of cellcular or 0. 8mldbs + ibdv of germinal and cellcular are higher than immune control group about 2 liters averagely. the chickens were inoculated with ibdv live vaccine mixed with the different dosages of lyophilized bursin by the eye drop method. the results sugest that cbs or dbs of different dosages can both improve the antibody inducation to different age chickens against ibdv. they may also alleviate the immunological injury of activated virus to bursa of fabricius. and promote the repairation of the lesion. it can be found that bs can raise body weight gain and feed coversion ratio
將超濾獲得的法氏囊活性肽分別以不同劑量肌肉注射21日齡spf雞，同時頸部皮下注射ibd胚毒或細胞毒滅活苗，結果表明：雞、鴨法氏囊活性肽都能夠縮短ibd油苗誘導產生抗體的時間，提高抗體水平，使雞可以在比較短的時間內獲得堅強的免疫。 0 . 4mlcbs胚毒組和0 . 8mlcbs細胞毒組或0 . 8mldbs胚毒和細胞毒組的agp抗體滴度平均比免疫對照組高2個滴度。將法氏囊活性肽與ibd活苗聯合免疫雞，結果表明：不同劑量的cbs和dbs都可以對不同日齡雞ibd抗體的產生有不同程度的促進作用；還可以減少弱毒對雞法氏囊組織的損傷，加快其修復。Bs of different dosages simultaneously inoculated rabbit with inactivated vaccines of rabbit hemorrhagic disease virus ( rhdv ). the hi liters of antibody against rdhv induced by rdhv inactivated vaccine in the group of high dosage bursin are higher than control group about 2 - 3 liters averagely. and the result of t - lymphocyte transformation assay shows that the bursin can temporarily enhance the t - lymphocyte transformation. the results of effects of bursin on body weight gain and feed conversion ration in rabbits show that bs can increase growth of mammal. the effect of bursin has a relation to its dosage, but has no ralation to the difference of interspecies and the method which come into the body
試驗中還發現， bs對雞體有一定的增重作用從而提高了飼料報酬。將不同劑量bs與兔瘟滅活苗同時免疫家兔，高劑量的dbs 、 cbs組可使兔瘟hi抗體水平較免疫對照組提高2 - 3個滴度。 t -淋巴細胞轉化試驗表明： bs可短暫促進t -淋巴細胞的活化。Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but
通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價，高親和力，較好特異性的抗mt單克隆抗體，最後獲得了5株單克隆細胞株，都能分泌針對mt的特異性抗體，建立了選擇性基團免疫分析法，用制備的抗體測定了人血清中mt的含量，作了mt的抑制標準曲線，其抑制范圍從50pg ? 30ng ，半抑制量為1By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。The who classification states that acute lymphocytic leukemia ( all ) should be classified by the pattern of reactivity of cell to a panel of lineage - associated antibodies and, where possible, genetic abnormalities. the expression of cd antigens on leukocytes is currently determined by flow cytimertry, which is expensive and labor - intensive, requiring 5 - 20ul quantities of fluores - cently labeled antibodies and allowing concurrent analysis for a limited number of cd antigens, usually three to four. from clinical point of view, an efficient method is required for analysis of a large number of samples in a single experiment
我們設計了一種新型的細胞晶元，其原理是充分利用玻片的硬度高，透明性好的特點，應用細胞膜表面不同的抗原物質，與包被在玻片上的不同抗體發生特異性結合，通過一次實驗可以將被測細胞懸液中不同膜表型的細胞分離、固定在同一張玻片的不同特定區域，保持了細胞的完整性及活性，又可通過簡單的操作一次將多細胞懸液進行免疫分群。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。. from the direct mutant of spirulina platensis ( sp - d ), we got high purity and activity phycobiliprotein which could grow crystals. the algae fluorescent probe prepared by coupling the above polyclonal antibody to phycobilipotein not only keeps the property of stronger anti - fluorescence quenching but also has the lower fluorescent background when it was used for labeling stoma cells of pea tendril
以原核表達的peac1為抗原制備了免疫活性較好的抗豌豆肌動蛋白的多克隆抗體，從螺旋藻中純化了高純度、高活性、能結晶的藻膽蛋白，將兩者偶聯制備的藻熒光探針，不僅保持了藻膽蛋白很強的抗熒光淬滅能力，而且用於豌豆卷須氣孔細胞熒光標記時有更低的熒光背景。When the antigen ( brief named ag ) invaded into the organism, and the body can produce a kind of material ( high molecular weight protein, named antibody, brief named ab ) that can distinguish this antigen and evacuate it, the antigen and antibody take place the immuno - reaction. using their highly sensitivity to response mass change and specificity, it can be fabricated a piezoelectric immunnosensors
當異種蛋白（稱為抗原， antigen ，簡稱ag ）侵入生物體內時，體內能產生識別此類異物並將其排出的物質（大分子量蛋白質，稱抗體antibody ，簡稱ab ） ，抗原與抗體發生免疫反應，利用抗體（或抗原）對抗原（或抗體）的特異性識別功能和壓電晶體的高靈敏質量響應可製成壓電免疫傳感器。To investigate the functions of these factors, cells were treated with anti - c - fos, anti - c - jun and anti - ras antibodies and examined microscopically for the cell cycle progression. the results indicated that c - fos - like protein, c - jun - like protein and ras - like protein played important roles in checkpoint regulation in physarum polycephalum. western blot analysis showed that the c - fos - like protein and c - jun - like protein may act in cell cycle checkpoint by changing the cyclin b1 - like protein and p53 - like protein expression ; and the ras - like protein may act by changing the cyclin bl - like protein, p53 - like protein, c - fos - like protein and c - jun - like protein expression
蛋白免疫印跡分析的結果表iv明，當s期、 gz期和前期細胞內具有功能活性的類ras蛋白的量減少時，細胞中的細胞周期調控因於類cyclinbi蛋白、類c jun蛋白、類c fos蛋白和類p53蛋白的表達水平，與未經抗體處理時相比發生了變化（或升高或下降） ；而山抗體處理引起的多頭絨泡菌細胞內具有功能活性的類c fos和類c jun蛋白的減少，使細胞內類cyclinbi蛋白和類p53蛋白的表達水平與抗體未處理時相比也發生了明顯的改變。The algorithm can effectively o vercome immature convergence phenomenon in simple genetic algorithm ( sga ). it can improve not only antibody ' s similarity but also its diversity. and it can avoid local optimal solution and shorten searching time
該演算法可以有效地克服基本遺傳演算法的未成熟收斂現象，既可以提高抗體的相似性又可以兼顧到抗體的多樣性，為避免演算法陷入局部最優解，縮短搜索時間提供了保證。He speculates that, for reasons as yet unknown, a mother ' s immune system takes note of the number of male offspring and that each succeeding male fetus is subjected to increased levels of antibodies
他推測，由於一些尚不為人知的原因，母體免疫系統可監測男性子代的數量，並且升高的抗體水平會影響每一個后孕男胎。分享友人