高度保守序列 的英文怎麼說

中文拼音 [gāodùbǎoshǒuxùliè]
高度保守序列 英文
highly conserved sequence
  • 高 : Ⅰ形容詞1 （從下向上距離大; 離地面遠） tall; high 2 （在一般標準或平均程度之上; 等級在上的） above...
  • 度 : 度動詞[書面語] （推測; 估計） surmise; estimate
  • 保 : Ⅰ動詞1 （保衛; 保護） defend; protect 2 （保持） keep; preserve; maintain in good condition 3 （...
  • 守 : Ⅰ動詞1 （防守; 看守） guard; defend 2 （守候; 看護） keep watch 3 （遵守; 遵循） observe; abide b...
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 高度 : altitudeheightelevation
  1. As analyzed, ( 1 ) the rapd technique is highly sensitive to investigating genetic diversity in t. lepturus and e. muticus. t. lepturus exhibits lower polymorphism and genetic diversity than e. muticus ; ( 2 ) according to the analysis of the partial mitochondrial 16s rrna gene sequences, a very low intraspecific variation and considerably high divergence among species were found, which reveals a dual nature of conservatism and variability in mitochondrial 16s rrna gene ; ( 3 ) five primers generate the species - speeific rapd sites and these sites can be served as the molecular markers for species identification and ( 4 ) it can be proved at dna variation level that t. lepturus and e. muticus are of two species respectively pertainiag to different genera, which supported the nelson taxonomic conclusion

    分析結果表明: （ 1 ） rapd技術研究黃海帶魚和小帶魚的遺傳多樣性具有較高的靈敏度和檢出率，帶魚的多態比例和遺傳多態度均較小帶魚的低; （ 2 ）線粒體165出兇a基因序列在分析兩物種遺傳變異時表現出保守和變異的雙重特性，種內變異極小而種間較大: （ 3 ） 5個隨機引物擴增出種特異的ra衛d帶，可作為種間分子鑒定標記; （ 4 ）研究證實帶魚和小帶魚是不同屬的兩個種，從而在分子水平上支持了nelson分類系統的觀點。
  2. Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis

    二、研究論文1 、參照人sry基因hmg - box保守區的序列，設計一對兼并引物， pcr擴增了中華絨螯蟹的sox基因，並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ，其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ，顯示該基因在進化上具高度的保守性。
  3. Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha

    與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較，二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區，都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點，所不同的是： （ ）在結構功能域上， 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區； （ 2 ）在功能位點上， 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區，但沒有n糖基化位點； （ 3 ）擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性（ e
  4. Which shared 10 % ~ 73 % identities to other rips from plants and 37 % ~ 73 % to other rjps from cucurbitaceae. six novel rip gene fragments ( 408 ' bp ), 1 from benincasa hispida and 5 from cucurbit a moschaia

    根據葫蘆科rip上、下游兩段高度保守的氨基酸序列設計簡並引物對ly1 ly2 ，對基因組dna進行pcr擴增，首次建立了rip新基因快速篩選體系。
  5. Was multiplied and the tk gene was cloned. the cloned tk gene was retrieved by proper restrictive hemodynamics. the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin. then, the specificity and sensitivity of tk gene probe were detected with dot blot hybridization. the sequence of tk gene of nm98a strain was analysed. the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99. 7 %

    本研究中首次對iltv - nm98a株的tk基因進行了克隆和序列分析，結果表明： iltv - nm98a株tk基因的核苷酸序列與已發表的iltvtk基因的核苷酸序列具有高度的同源性，兩者之間僅相差4個核苷酸，同源性高達99 . 7 ，從而證實了iltvtk基因是高度保守的，為iltvtk基因核酸探針的制備提供了有力的依據。
  6. To investigate the secondary structure of this gene, we found it has a transmembrane region near the n - terminus, followed by a proline - rich conserved region, and it has a conserved haemachrome binding region about 60 aa away from carboxyl terminus

    分析氨基酸序列的二級結構發現基因n -端具有跨膜的疏水序列，其後具有富含脯氨酸保守區，在距離羧基端60個氨基酸處具有高度保守的血紅素結合域。
  7. Hla - g1, which is a newly defined non - classical hla class i molecule, plays an important role in mediating immunotolerance and protecting embryo and even some kinds of tumors from nk cells attacking. the full - length coding sequences containing cdna of hla - g1 were cloned from placenta, monocytes and liver cancer tissue of chinese donors. sequence analysis reveals that it is a highly conserved human gene with only two amino acid mutation sites compared to foreign nationality. its truncated form was overexpressed in

    從中國人外周血單個核細胞胎盤組織和肝癌組織等樣品中克隆了包含完整hla - g1讀框的cdna與國外同行獲得的該基因及其蛋白質序列比較分析表明，該基因雖然有著細微的種族特異性，但高度保守並獲得了它的截斷型重組蛋白，根據蛋白一級結構和同源比較方法，模建了它及其與特異性受體kir2dl4形成復合體的空間結構模擬，預測了它們之間相互作用的特徵。
  8. Highly conserved sequence

    高度保守序列
  9. In order to form a chloroplast transformation system of d. salina, we have conducted some studies including its sensitivity to antibiotics, the activity of promoter, cloning of the chloroplast genes and construction of transformation vectors, so far a pilot transformation system of the d. salina chloroplast has been completed. methods : the sensitivity of d. salina to seven antibiotics or herbicide used commonly in gene engineering was studied and the biological activity of atpa promoter from c. reinhardtii chloroplast was tested by using enhanced green fluorescent protein ( egfp ) as a reporter. primers were designed in the conservative encoding regions according to the chloroplast genomes from four algae which have close genetic relationship with d. salina, and the sequences of 16s rrna, chll and chln of d. salina chloroplast were cloned and sequenced, respectively

    方法:根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，在基因編碼區的高度保守區域設計引物，克隆了杜氏鹽藻葉綠體165識na基因、咖l基因和ch n基因，並分別以165識na基因和chln基因序列為同源片段，以cat和bar基因為篩選標記基因構建了三套杜氏鹽藻葉綠體轉化載體: 2鄭州大學2003年博士學位論文杜氏鹽藻（ d ~ iiellasalina ）葉綠體轉化研究pds165一eaf 、 ptn1269一bar和psp72一5一bar一3 ，用基因槍法轉化杜氏鹽藻，初步建立起杜氏鹽藻葉綠體轉化體系。
  10. Dna sequence analysis showed that the cloned nk gene was highly homologous to the sequence reported by nakamura. the deduced amino acid sequence also had the conserved sequences ( serine 221, histidine 64, and aspartic acid 32 ) which was essential for the catalytic center of serine proteases. the nk gene was then cloned into the transfer vector pvl1393

    本實驗以b . subtilis （ natto ）基因組dna為模板擴增了nk基因，測序結果顯示與nakamura報道的納豆激酶基因高度同源，其氨基酸序列也含有絲氨酸蛋白酶的活性保守中心（ serine221 ， histidine64 ， asparticacid32 ） 。
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