Based on recent molecular phylogenetic analyses using nucleotide sequences of the encoding the large subunit of ribulose 1, 5 - bisphyosphate carboxylase / oxygenase ( rbcl ), hypodematium should be not included in the athyriaceae, it has closely related to dryopteridaceae. on the other hand, athyriaceae, thelypteridaceae, blechnaceae, onocleaceae and woodisaceae form a large clade, so it may explain that tryon & tryon ( 1982 ) and kramer & kato ( 1990 ) putting it forward as dryopteriaceae s. 1
運用cpdna基因組編碼的磷酸核酮糖羧化酶大亞基（ rbcl ）的基因
序列測定而構建的系統樹，顯示蹄蓋蕨科、金星蕨科、烏毛蕨科以及其他科構成一條與
鱗毛蕨科平行的分支，因此可以說明kramer & kato （ 1990 ）把蹄蓋蕨科放入廣義的
鱗毛蕨科是不合理的。
On the basis of image segmentation, this thesis mainly discusses the algorithm of automatic target tracking in sequence images on the sea. firstly, in terms of the characteristics of image on the sea, such as weak contrast between target and background, borderline between sky and sea, spoondrift and so on, a method called barycenter tracking which is based on intra - frame information from the sequence images is proposed, in which the threshold method is the key algorithm
在圖象分割演算法研究的基礎上，本文重點從兩方面討論了海上
序列圖象中運動目標的自動跟蹤演算法:首先，針對海上圖象的特點，即背景與目標間的弱對比度、存在海天線、魚
鱗光和目標拖尾等，設計了以閾值分割為核心的基於幀內信息的形心跟蹤方法。
In this paper, total dna from tannage, tail skin, scales and the skin treated with salt were successfully extracted with an improved method for dna extraction. to verify the results, four pairs of primers, which were universal primers for 12s rrna gene, diagnostic primers of chinese alligator meat, microsatellite primers and rapd primer, were used to do pcr amplifications. some amplified fragments were sequenced, either
本文研究出一種改進的dna提取方法，成功地從揚子鱷鞣製皮革中提取了總dna ，同時對不同的組織標本如
鱗片、鹽腌生皮及尾尖皮等進行了dna提取：並利用12srrna基因擴增的通用引物、揚子鱷鑒定性引物、微衛星引物及rapd引物進行pcr擴增，且對部分pcr擴增結果進行測
序，以檢驗提取效果。
The association of larval and adult stages of ecologically important caddisfly ( insecta : trichoptera ) using mitochondrial dna sequences larval and adult amplification fragments were cloned and sequenced. dna sequences were aligned using clustal x software ( gene codes corporation )
線粒休dna一coi 、 u基因
序列在毛翅目成幼蟲聯系中的應用研究擴增並測定了4種
鱗石蛾的成、幼蟲的mtdnacoi 、 coh及trna一leu基因
序歹『 j 。
Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸
鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸
鱗蝰素（ echistatin ）的氨基酸
序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
The role of cytochrome oxidase i gene and its fragments on phylogenetic reconstruction of 4 lepidostomadon it is well known that the phylogenetic trees reconstructed from different genes for the same set of organisms are often different
二、線粒體col
序列不同區段在4種
鱗石蛾系統樹重建中的作用將coi
序列按其堿基位點分成第430一779 、第780一1179和第1180一1569位
Confidence levels for nj tree were assessed by bootstrapping from 1000 pseudo - replications
對
鱗石蛾8個coi和co11
序歹
Squamous cell carcinoma was dominant, followed by undifferentiated cell carcinoma, olfactory neuroblastorma and malignant lymphma
病理以鱗狀上皮癌居多，其次依序為分化癌、嗅覺神經母細胞癌、惡性淋巴瘤等。
