鼠單克隆抗體 的英文怎麼說
中文拼音 [shǔdānkèlōngkàngtǐ]
鼠單克隆抗體
英文
monoclonal antibodies-
Mg7, a murine mab against human gastric cancer, was prepared and proved to possess quite high specificity and sensitivity in recognize to an ascertained antigen associated with gastric cancer in the previous studies of our lab
Mg _ 7單抗是我所研製的一株小鼠抗人胃癌單克隆抗體，既往研究表明單抗對其相應抗原的識別具有較高的特異性和敏感性。The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds. the last round enriched phage clones were used to reinfect
由鼠源噬菌體抗體庫淘選到展示有人絨毛膜促性腺激素單鏈抗體的噬菌體克隆。Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but
通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價，高親和力，較好特異性的抗mt單克隆抗體，最後獲得了5株單克隆細胞株，都能分泌針對mt的特異性抗體，建立了選擇性基團免疫分析法，用制備的抗體測定了人血清中mt的含量，作了mt的抑制標準曲線，其抑制范圍從50pg ? 30ng ，半抑制量為1Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。Ptxb1 - hng and ptxb1 - m - insulin are expressed in e. coli successfully. after sds - page and densitometric scan analysis, the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )
Pbv220 ? hng在大腸桿菌中未檢測到表達，后兩個克隆在大腸桿菌bl21 （ de3 ）中獲得高效表達， hng及m - insulin融合蛋白表達量分別佔全菌蛋白的40及50左右；經western - blot鑒定m - insulin融合蛋白可以與小鼠抗人胰島素單克隆抗體（ igg ）發生抗原抗體結合反應。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。Anti - cdc25c rabbit polyclonal antibody was purchased from santa cruz biotechnology, anti - cdc2 and anti - cyclinbl mouse monoclonal antibody were from neo - markers, anti - cdc2 ptyrls monoclonal antibody was obtained from new england biolabs
免疫印跡分析用抗體： cdc25c免多克隆抗體購于santacruz公司。 cdcz ，周期素m鼠單克隆抗體購于neomarkers公司。Pathological, biochemical and molecular biological technique were used to study the changes of bcl - 2, bax, caspase - 1, caspase - 3 and nf - kb after brain contusion aim to investigate the relationship among them and apoptosis and try to provide some objective marker and theory basis to the judgment on post - traumatic intervals and severity of brain injury
實驗材料與方法實驗材料1 .實驗動物健康wistar大鼠54隻，雌雄不限，體重1809一2809 ，由中國醫科大學實驗動物中心提供。 2 .主要試劑.鼠抗人bel一2 （ e一2 ）單克隆抗體（產品編號: s 。Amplification, cloning and sequence analysis of the light chain and the heavy variable region genes of monoclonal anti - idiotypic antibody np30 of schistosoma japonicum to amplify and sequence the gene of the heavy chain and the heavy chain of anti - idiotypic monoclonal antibody np30 of schistosoma japonicum
2 、日本血吸蟲單克隆抗獨特型抗體np30的單鏈抗體kcfv ）對balb c小鼠誘導保護性作用研究為了研究日本血吸蟲抗獨特型抗體np30單鏈抗體作為日本血吸蟲病Pcr detection found there were 1. 2x104 tfu in the library. one of colonies of scfv antibody, which binds to bull sperm, was selected and sequenced. sequence analysis indicates that the scfv colony resembles the mouse antibody
用phage - elisa從文庫中篩選並鑒定出一個針對牛精子的陽性克隆，序列分析發現，該單鏈抗體克隆的序列符合小鼠抗體可變區的一般特徵，這說明構建的針對牛精子的噬菌體抗體文庫是成功的。Effect of genetic contamination of balb c mouse on the preparation of monoclonal antibodies
小鼠遺傳污染對單克隆抗體制備的影響分享友人