In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明,該病毒為rna病毒,對氯仿、乙醚敏感;胰酶試驗中,經37 、 1小時處理的病毒,仍然能夠在貓源細胞fcwf細胞上生長,並且毒力基本保持不變;耐酸性試驗中,病毒分別在ph5 . 0和ph3 . 0經37作用2小時,毒力僅下降一個滴度;耐熱性試驗中,該病毒在恆定溫度50 ,設定不同時間,從5分鐘到150分鐘,毒力均有不同程度下降,其中, 50作用30分鐘,病毒平均滴度下降2個單位; 50 , 60分鐘, cpe消失;恆定時間1小時,設定不同溫度( 50 - 60 - 70 - 80 ) ,病毒在細胞上
完全喪失增殖能力, cpe消失。生物學試驗,利用實驗室現有條件,選擇不同的細胞系對該病毒進行培養,發現該病毒對貓源細胞fcwf最敏感; mdck細胞次之; f81細胞經多次傳代,亦可出現cpe ;而vero細胞則不敏感。血凝試驗表明,該病毒對豬、雞、人及豚
鼠的紅細胞均無血凝性。
The open reading frame of tsarg2 was obtained from human testis cdna library by pcr. using in situ hybridization on tissue section of human testis. we preliminarily studied the expression and function of tsarg2
從小
鼠睪丸cdv文庫中分離出該基因
完整閱讀框cdna , ; hu基因的cdna全長為1088hp ,包含6個外顯于,基因組跨越9
Mouse operation, using glass of beer barrel fight then handed guests, guests will be drinking cup into the air pool cleaning
鼠標操作,拿杯子到啤酒桶打啤酒然後遞給客人,將客人喝
完的空杯放到水池清洗。
We found nuclear deformation lymphocytes with increased heterochromatin and impaired thymus epithelium cells with increased lysosomes and deformation of mitochondrias. ( 4 ) effects on mouse liver of so2 challenge : so2 can cause significant liver injury. he staining showed several kinds of necrosis of liver including spot necrosis, focal necrosis and submassive necrosis infiltrated with lymphocytes, monocytes, few neutrophils and eosinophils ; tem observation showed fatty degeneration with dispersion of fatty droplets and dilation of rough endoplasmic reticulums, acid degeneration with significant hyperplasia of mitochondrias, necrosis of hepatocytes with karyorrhexis and other organelles losing their normal structure
( 4 )二氧化硫染毒對小
鼠肝臟的組織學結構有明顯影響,可引起肝臟點狀壞死、灶狀壞死甚至片狀壞死,伴隨不同程度的炎性細胞浸潤;透射電鏡觀察發現二氧化硫可引起肝細胞脂肪變性、嗜酸性顆粒變和壞死,脂肪變肝細胞中可見大小不等的脂滴存在,嗜酸性顆粒變肝細胞中可見線粒體明顯增生,壞死肝細胞可見細胞核結構破壞,細胞器減少,細胞膜不
完整。
1987, 30 min, a four - part series inspired by tchaikovsky s fourth symphony op. 36 of the same name, begins with a segment starring mosa the flying squirrel that bridges the gap between early crude animation and the works of disney
:柴可夫斯基第四交響曲同名作品啟發,寫前迪士尼迪士尼與后迪士尼三階段的動畫史,手冢超過十年的嘔心瀝血,也只能
完成第一與第四段,第一段飛行松
鼠莫沙以土法炮製,靠剪輯戲法變出活動畫面。
1987, 30 min, a four - part series inspired by tchaikovsky s fourth symphony op. 36 of the same name, begins with a segment starring mosa the flying squirrel that bridges the gap between early crude animation and the works of disney. with the second and third episodes never finished, it then concludes with a richly expressive forest being driven out by limited made - for - tv animation
《森林傳奇》第一集( 1987 ,彩色, 30分鐘) :柴可夫斯基第四交響曲同名作品啟發,寫前迪士尼、迪士尼與后迪士尼三階段的動畫史,手冢超過十年的嘔心瀝血,也只能
完成第一與第四段,第一段飛行松
鼠莫沙以土法炮製,靠剪輯戲法變出活動畫面。
The pine tree project has completed extensive environmental impact studies to monitor and protect threatened and endangered species including the desert tortoise and the mojave ground squirrel
松樹項目已經
完成了廣泛的環境影響研究,以監測和保護可能受到威脅和危險的生物物種,包括沙漠龜和莫哈韋地面松
鼠等。
Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed
摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此,人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用
完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小
鼠、大
鼠、倉
鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。
Because it well reserves the chemical stability of inorganic glass, it has the following features : low density, low thermal conductivity, vapor impermeability, no water absorption, incombustibility, protection from mould and rat eating, high mechanical strength but easy to cut and able to bear all kinds of chemical erosions except the hydrofluoric acid
由於它
完全保留了無機玻璃的化學穩定性,具有容重低導熱系數小不透濕不吸水不燃燒不霉變不受
鼠嚙機械強度高卻又易加工,能耐除氟化氫以外所有的化學侵蝕。
Pay attention to the first and the last pics, how did that long stalk become a japanese stalk from a mousetail
注意到第一張圖和最後接近
完工的圖里,長的那把刀莖怎麼從老
鼠尾巴變成了日式莖了呢?
We took the scroll wheel one step further with an eye - catching translucent look and a wider design, which makes it fun to scroll through files
使用這種新式滑
鼠將為您帶來更多樂趣,更寬且更靈敏的滾輪可以更快速地瀏覽文件,
完全不需要使用卷動軸。
Copying and dragging spirographs completed spirographs can be dragged to different locations on the drawing area using the mouse
可使用
鼠標將
完成的螺旋形拖動到繪圖區的不同位置。
Mr. and mrs. kangaroo and their son enjoyed their evening dinner. after the dessert, they all threw spitballs at each other across the dinning - room table
袋
鼠夫婦和他們的兒子津津有味地共進了晚餐。吃
完甜點后,他們在飯桌旁互相扔起紙團來。
From threadworms to mice, putting an animal on a diet that is near, but not quite at, starvation point prolongs life - sometimes dramatically
讓線蟲或小
鼠定量飲食,使其接近饑餓但又不
完處于饑餓狀態時可以延長它們的生命,有時效果非常顯著。
It s not easy satisfying a room full of hungry patrons as you wait on drive - thru customers and take reservations over the phone, but with snowy s musical talent and help from the dessert tray, you might survive the shift
第一關為訓練關,即使沒玩過同類游戲的玩家,也很容易掌握。點餐上菜結帳翻臺,可以一「
鼠」
完成。
The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment. in brief, the intact blastocysts were plated on sto feeder layer treated with mitomycin, and were cultured in the media supplymented with brl condition medium
聯合evans和martin的方法,稍加改良來分離小
鼠胚胎幹細胞,把昆明白小
鼠完整的囊胚直接種植在經絲裂黴素滅活的sto飼養層細胞上,在含有brl條件培養基的es細胞培養液中培養。
On spatial memory and calcium homeostasis of oxidative damaged ad rats
昆明小
鼠完成放射狀水迷宮和嗅覺辨別任務時年齡和性別的差異
All groups of microtus fortis showed polymorphism at most of the ten loci investigated. the electrophoretic mobilities of the small voles from heilongjiang revealed great divergence with the other three groups : the small voles from heilongjiang had their particular alleles at six loci ( es - 1, hbb, gpi, akp - 1, trf - 1, ce - 2 ) and the phenotypes of them were completely different at three ( es - 1, hbb, gpi - 1 ) of these loci. the genetic distances among the voles from hunan, ningxia and the big voles from heilongjiang were ranged from 0. 0633 to 0. 2107 while that between the small voles from heilongjiang and the other three groups were ranged from 0. 7068 to 0. 8953
結果表明:四類東方田鼠在所觀察的10個生化位點上,大部分都存在著多態性現象;黑龍江小體型東方田鼠與其它三類東方田鼠的電泳結果差異較大,在es - 1 、 hbb 、 gpi - 1 、 akp - 1 、 trf 、 ce - 2六個位點土都存在其所特有的等位基因,其中es - 1 、 hbb和gpi - 1三位點上它的基因型與其它三類鼠完全不同;湖南、寧夏和黑龍江大體型東方田鼠三者間的遺傳距離在0 . 0633 0 . 2107之間,而黑龍江小體型東方田鼠與其它三類鼠的遺傳距離在0 . 7068 0 . 8953之間。