鼠胚細胞 的英文怎麼說

中文拼音 [shǔpēixìbāo]
鼠胚細胞 英文
embryo mouse cell
  • 鼠 : 名詞（老鼠） mouse; rat
  • 胚 : 名詞[生物學] （初期發育的生物體） embryo
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. Chromosome analysis confirmed that blastocyst cells were derived from es donor cell

    重構囊胚細胞經染色體分析，證實其染色體來源於小鼠胚胎幹細胞。
  2. The protein nucleic acid complexes were identified by autoradiography for 48 h. the psg5heo and igfbp - 6 promoter pcat vectors were transfected into cos - 7 cells with iipofectamin2000 ; these cos - 7 cells where treated with 1 jxm e2, with lonm tcdd and with both lonm tcdd and 1 jxm e2, individually ; the cat protein was examined by cat - els a. results : the congenital skeletal defects in fetal rats were induced with 10 - 15ug / kg tcdd or with 100 - 140 mg / kg ra with dosage - dependence effect. the igfbp - 6 mrna in rat calvaria tissue and mc - 3t3 - el cells were increased with tcdd and ( or ) ra, whereas the cell proliferation and igf - 2 mrna in mc - 3t3 - el cells was decreased in the status of e2

    結果: tcdd在大鼠胚胎發育時期具有骨骼致畸作用，骨骼發育畸形包括:內翻足、脊柱裂、顱面骨發育缺陷、無尾畸形等;隨著tcdd濃度的升高（ 10一15林擴kg ） ，致畸率明顯上升（ 14 . 7 %一24 . 6 % ） ; tcdd和ra聯合用藥可以增強對大鼠骨骼發育的致畸效應; e :對成骨細胞內igf一2基因有正性調節作用，對igfbp一6基因有負性調節作用， tcdd可以抑制e :對細胞內xgf一2和igfbp一6的調節作用。
  3. Like many embryonic cells, myoblasts from prenatal rats or chicks are easy to culture.

    正如許多胚胎細胞一樣，胚胎大鼠或雞的成肌細胞是易於培養的。
  4. Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded

    方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。
  5. Mouse ; restriction fragment length polymorphism ; dna fingerprint ; probe jl - 02 ; es cell

    小鼠限制性片段長度多態性dna指紋圖jl - 02探針胚胎幹細胞
  6. A primary culture of myoblasts may be prepared by excising skeletal muscle tissue from 10 to 30 day old rat embryos.

    制備成肌細胞的初級培養物，可以將10-30天的大鼠胚胎骨骼肌組織剪切下來。
  7. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  8. Protein kinase a is camp - dependent kinase, one of the most important signal transduction pathways, plays a pivotal role in growth, differentiation, tumor occur, cell cycle control, etc. pka activators such as camp, 8 - br - camp or phosphodiesterase ( pde ) inhibitor isomethyl butyl xanthine ( imbx ) or purified pka catalytic subunit all can inhibit germinal vesicle breakdown ( gvbd ) and meiotic maturation in mouse oocytes, also in xenopus oocytes

    蛋白激酶a （ proteinkinasea ， pka ）是依賴于camp的絲蘇氨酸蛋白激酶，是重要的信號傳導途徑之一，廣泛參與許多生命過程，包括生長、分化、腫瘤發生、細胞凋亡、細胞周期調控等。以小鼠卵母細胞為實驗對象，給予pka激動劑camp 、 8 - br - camp或磷酸二酯酶抑制劑imbx （ isomethylbutylxanthine ）均可抑制小鼠卵母細胞的胚泡破裂及減數分裂成熟。
  9. Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed

    摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此，人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。
  10. The purpose of this study was to establish es or eg cells lines in the future for further research. the results attained were as follows : 1. derivation of embryonic stem cells from icr mouse preimplantation embryos

    本試驗對icr小鼠胚胎多能幹細胞的建系工作做了初步探索，為胚胎多能幹細胞多種屬廣泛建系的研究打下一定基礎，也為小鼠胚胎多能幹細胞的深入研究積累經驗並提供借鑒。
  11. 2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage

    Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料，經傳代培養，獲得能連續而穩定傳至6代的，具有胚胎幹細胞諸多特性的eg細胞系。
  12. ( 3 ) isolation and culture of human primordial germ cells ( pgcs ). human pgcs collected from gonadal ridges and mesenteries were grown on mouse feeder layers in the presence of human recombinant leukemia inhibitory factor ( lif ), human recombinant basic fibroblast growth factor, and forskolin as described previously. initially, pgcs were visualized by alkaline phosphatase activity staining

    （ 3 ）人類pgcs的分離和培養從4 10周齡藥物流產胚胎的生殖嵴和腸系膜組織中分離原始生殖細胞（ primordialgermcells ， pgcs ） ，培養在添加人重組白血病抑制因子（ lif ） 、人重組堿性成纖維細胞生長因子（ bfgf ）和forskolin的小鼠飼養層細胞上。
  13. The research of relationship between the kidney in the sense of tcm and mesoderm from the influence of tonifying the kidney on apoptosis of fibroblast cells of kidney - deficient rats

    從補腎對腎虛大鼠成纖維細胞凋亡的影響看中醫腎與中胚層的相關性
  14. The effects of selenium on growth and differentiation of cul - tured neuron from rat embryo midbrain

    硒對大鼠胚胎神經細胞增殖和分化的影響
  15. Experiment i study on the embryonic development by injecting different developmental stage embryos. this experiment collected 109 embryos to observe their developmental ability by es cells microinjection into 8 - 16 cell stage morula or blastocyst

    本試驗先後將109枚胚胎用於此項研究，通過將小鼠es細胞注入8 - 16細胞桑椹胚和囊胚，觀察兩個不同時期注射胚胎的發育能力。
  16. Objective the present study was to investigate the effects of endotoxin on capacitation and acrosome reaction ( ar ) of mouse, golden hamster and human sperm, on the sperm - oocyte fusion in mouse, and on the development of 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos in vitro. the purpose was to definite the committed step and the mechanism during in vitro fertilization ( ivf ) on which endotoxin affected, and to distinguish the sensitivities to endotoxin of three developmental systems of mouse embryos. all these data would provide the reference to clinical and laboratory quality control

    目的研究內毒素對小鼠、金黃地鼠及人精子的體外獲能和頂體反應、小鼠精卵結合及小鼠1 -細胞、 2 -細胞和去卵透明帶2 -細胞胚胎體外發育的影響，探討內毒素影響體外受精結局的環節及可能的機制，確定三個體外培養系統對內毒素的敏感性，以期為臨床和實驗室質量控制提供參考。
  17. To select the best mouse blastocysts resulting from mm16, mm16 + e2, mm16 + p and mm16 + e2 + p mediums, and from ivf - ivc ( in vitro fertilization and in vitro culture ) in htf medium, and from in vivo was for embryo transfer

    分別選取小鼠1 -細胞胚胎在mm16 、 mm16 + e2 、 mm16 + p 、 mm16 + e2 + p各組培養液中培養的囊胚、小鼠體外受精-體外培養的囊胚和體內正常發育的囊胚，進行子宮內移植。
  18. In conclusion, so2 can not only affect respiratory system, but also other organs such as spleen, liver, thymus on their histological structures and every organ performs very differently under so2 exposure. so2 derivatives has cytotoxicity both on mouse spleen cells and human embryo lung fibroblasts

    說明二氧化硫代謝衍生物對人胚肺成纖維細胞和小鼠脾細胞都有一定毒性，並且在一定范圍內對小鼠脾細胞具有一定的凋亡誘導作用。
  19. Day 4 embryoid body ( 4deb ) cells were derived from es cells and then induced with mbmec - cm into hematopoietic precursor cells. immunocytochemistry staining and flow cytometry were adopted to observe the antigen expressions. rt - pcr method was used to detect the expressions of hematopoietic genes

    在誘導小鼠es細胞向造血細胞分化的實驗中，我們採用先將小鼠胚胎幹細胞形成4天擬胚體（ day4embryoidbodies ， 4debs ） ，再用骨髓內皮細胞條件培養液誘導4天擬胚體細胞生成造血干祖細胞。
  20. We used the icr and kunming mice and got the embryos of 3. 5dpc by means of superovulation, then cultured the embryos on the feeder layer derived from icr or kunming mice, dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time. in this period, we analized the effects of feeder layer, trypsin and embryos isolated from uteri of different varietal mice on the es cell lines

    以icr小鼠和昆明小鼠為實驗對象，採用超數排卵的方法獲得小鼠胚胎，培養在用絲裂黴素c處理過的icr小鼠和昆明小鼠mef細胞飼養層上，選取適當時間離散增殖的icm和類es細胞集落，分析了在小鼠es細胞建系過程中，飼養層細胞、消化液及不同品系來源的胚胎的影響。
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