a-elisa 中文意思是什麼

a-elisa 解釋
放大酶聯免疫吸附試驗
  • a : an 用在以母音音素開始的詞前〉 indefinite art 1 〈普通可數名詞第一次提到時，冠以不定冠詞主要表示類...
  • elisa : 埃莉薩
  1. The result of elisa displayed that the all the serum against cdv and cav, and immunoglobulin against cav had high activity. but the igg against cdv did n ' t appear a high activity

    結果表明狐抗cdv 、 cav高免血清和雞抗cdv 、 cav卵黃的活性都較高，但提純的抗cav的狐igg和雞igy的活性較高，而提純的抗cdv的狐igg和雞igy的效價較低。
  2. From the milkhouse there was the steady thump, thump of a churn being manipulated by the half-witted girl, elisa stoughton.

    從奶牛棚里響起了傻大姐艾利莎斯托頓調弄攪乳器的一成不變的錚錚之聲。
  3. From the milkhouse there was the steady thump, thump of a churn being manipulated by the half - witted girl, elisa stoughton

    從奶牛棚里響起了傻大姐艾利莎?斯托頓調弄攪乳器的一成不變的錚錚之聲。
  4. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  5. 2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody

    選其中一株3 - h _ （ 11 ）做雜交-雜交瘤細胞染色體計數，同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數：制備腹水型和上清型抗體，用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體，即紅細胞固相吸附法測雙特異中文摘要性抗體，紅細胞凝集試驗測單特異性抗人a型紅細胞抗體，間接elisa法測抗p24抗體；用腹水型抗體做耐熱性及耐凍融實驗。
  6. The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test

    經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。
  7. From dead chicken which infected infectious stunting syndrom of our province, one virue was isolated using spfeggs, chicken embryo fibroblast, mdck18, and vero cell. this virus was unable to agglutinate chicken erythrocytes. in order to definite the pathogeny of infectious stunting syndrom. physical and chemical specific property, types of the nucleic acid of the isolated virus, recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken

    為確定該病的病原，對所分離病毒進行了理化特性測定、病毒核酸型別測定、動物回歸試驗等生物學特性測定，證明該分離病毒與細小病毒科（ parvovirdae ）細小病毒屬（ parvovirus ）的雞細小病毒（ chickenparvovirus ）特性基本相符，核酸型為dna型。
  8. A simple blood test called elisa is used to diagnose fiv, and this test can be done in most veterinary clinics or hospitals

    Elisa是一個簡單檢測fiv的方式，在大部分的動物醫院都可以做
  9. The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8. vectors were amplificated in the e. coli dh5 a and were linearized with bgl ii. the linearized vctors were transformed into host strain gs115. the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa. the recombinated protein was detected with sds - page and western - blot as before

    重組菌用甲醇誘導表達，用dot - elisa的方法篩選到表達量較高的菌株。將篩選出的菌株大量的誘導表達，對表達上清處理后，用sds - page和western - blot進行鑒定。同時，用hiprep16 10heparinff肝素親和柱對表達蛋白進行了初步的純化。
  10. Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan, and be stimulated by ifn - r before oligochitosan added, then measured the changes of gene transcription and translation level of both il - 1 and imf - a, respectively by methods of relatively quantitive rt - pcr and elisa. first, rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan, then by the same method, confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating. because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone, so add ifn - r to microphages alone for 22 hours, then examined by rt - pcr, the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group

    此外，由於ifn y單獨作用也可促進兩種細胞因子基因表達，故在巨噬細胞中加入ifn y單獨作用22h ，再經阿一pcr檢測，發現加ifn y的實驗組細胞的幾一lp和tnf a基因轉錄水平與空白對照組相比較無顯著性差異，可見，殼寡糖和ifn v對巨噬細胞il lp和tnf一口基因轉錄水平的影響在作用時間上無一致性，在殼寡糖作用最適時間時，僅受ifn y刺激的巨噬細胞il lp和tnf q基因轉錄己下降至刺激前水平，因此可以認為， ifn y的加入僅起到對巨噬細胞預刺激使之處于敏感狀態的作用，有利於增強殼寡糖對巨噬細胞的作用。
  11. The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months, the sensitivity and specificity of the diaphragm did not change, so it has good stability ; when 10 positive serum was detected 3 times, the result is reproducible, so the diaphragm has good reproducible. serums from experimental inoculated piglets was detected. the results showed that when the titer is l : 16, the pigs were infected with streptococcus suis ; and when 1 : 64, the pigs could survive after challege with streptococcus suis. all the results have shown that dot - ppa - elisa was a convenient, rapid, sensitive specific useful method for the detection of antibody

    該法以硝酸纖維素膜為固相載體，包被膜載抗原製成的診斷膜片具有良好的特異性：不與仔豬副傷寒、豬巴氏桿菌病、豬大腸桿菌病、豬衣原體病、豬瘟、豬細小病毒病、豬偽狂犬病、豬布氏桿菌、豬丹毒陽性血清反應；膜片具有良好的靈敏性，陽性血清作2 ~ （ - 11 ）稀釋亦能檢出；膜片具有良好的穩定性，在4至少能保存7個月，其靈敏性不變。
  12. Colibacollosis serotype k. 99 positive sera in ducks and duck plague positive sera were tested, whereas, the results of detection to r. a. sera were positive. the sensitivity of indirect elisa were 50 to 100 times ( serotype 1 ), ( 2 ) 25 to 100 times ( serotype 2 ), ( 3 ) 12 to 100 times ( serotype 4 ) and 25 to 200 times ( serotype 5 ) th an micro - agglutination test

    包被鴨疫里默氏桿菌抗原時，對鴨抗5 ： a多殺性巴氏桿菌陽性血清、鴨抗型鴨病毒性肝炎陽性血清、鴨抗鴨源大腸桿菌k _ （ 88 ）陽性血清、鴨抗鴨源腸炎沙門氏菌陽性血清、鴨抗鴨源大腸桿菌k _ （ 99 ）陽性血清、鴨抗鴨瘟陽性血清檢測結果呈陰性。
  13. It revealed a negative reaction with positive sera of prv hcv, prrsv, jev and sa215. it revealed a positive reaction with prv standand positive serum. the results showed that the established ge - elisa has the advantages shch as high sensibility strong specificity and good repeatability and can be used to differentiation of prv infection from vaccination

    與豬細小病毒、豬瘟病毒、豬乙型腦炎病毒、 prrsv的標準陽性血清呈陰性反應，與偽狂犬病病毒標準陽性血清呈陽性反應，與三基因缺失疫苗株sa215免疫豬所采血清呈陰性反應。
  14. 107 + 2. 819x ( serotype l ), log [ et reciprocal ] = 1. 019 + 2. 935x ( serotype 2 ), ( 3 ) log [ et reciprocal ] = 0. 99 + 2. 709x ( serotype 4 ) and log [ et reciprocal ] = 1. 052 + 2. 953x ( serotype 5 ). the derived regression lines ( equations ) were used to transfer the optical density value ( od ) obtained from a single 1 : 100 dilution of any unknown serum sample of duck into elisa titer. the indirect elisa technique were used to monitor the variety of the mean antibodies titers of vaccinated ducks with the oil - emusified formalin - inactived r. a

    間接elisa方法的靈敏度是微量凝集試驗的50 - 100倍（血清1型鴨疫里默氏桿菌） ， 25 - 100倍（血清2型鴨疫里默氏桿菌） ， 12 - 100倍（血清4型鴨疫里默氏桿菌） ， 25 - 200倍（血清5型鴨疫里默氏桿菌） 。
  15. No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract

    將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。
  16. By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq

    然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。
  17. By using western - blot, the fusion protein could not be react with antiserum to prrsv, whereas it presented a reactivity with swine antisera against prrsv and mab ge3 by elisa the results implied that the epitope might be one conformational epitope that was mainly composed of aa50 ~ aa55 domain on n protein

    Western - blot分析表明表達產物與prrsv陽性血清沒有反應性，而elisa分析表明表達產物可與prrsv陽性血清和單克隆抗體發生反應。由此說明單克隆抗體ge3所識別的抗原表位可能是存在於n蛋白上以kphf為核心的構象型表位。
  18. A dot - ppa - elisa has been developed to detect the specific antibody against streptococcus suis. the antigen was isolated from group c d e r and type 2 streptococcus suis by three different method : ( a ) autoclave extraction method, ( b ) hcl - extraction method, and ( c ) fuller ' s method

    本研究選取c 、 d 、 e 、 r群及2型鏈球菌標準菌株，以高壓法提取抗原建立了檢測豬鏈球菌病血清抗體的dot - ppa - elisa方法。
  19. A dot - enzyme - linked immnosorbent assay ( dot - elisa ) for the detection of antidodies against newcastle diesease virus ( ndv ) was established by using purified ndv and self - made enzyme - labeled anti - chicken igg, the mehod was also evaluated through it ' s application

    本研究用提純的新城疫病毒和自製的酶標抗體建立了檢測雞新城疫抗體的dot - elisa方法。試驗中用f _ （ 48 ） e _ 9株和lasota株提純抗原並對兩者進行了比較。
  20. Following the purification of the expressed protein, a elisa was developed to detect reference antisera of h7, h5 and h9 subtypes. the result indicated that h7 antisera was positive, h5 and h9 antisera were negative

    用電洗脫方法純化菌體表達ha1蛋白后，建立了間接elisa方法，並對aivh7 、 h9 、 h5亞型血清進行檢測，結果h7亞型血清呈陽性， h5 、 h9亞型血清呈陰性反應。
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