a-form dna 中文意思是什麼

a-form dna 解釋
a-型dna
  • a : an 用在以母音音素開始的詞前〉 indefinite art 1 〈普通可數名詞第一次提到時，冠以不定冠詞主要表示類...
  • form : n 1 形態；形狀；樣子，外貌；【哲學】形式 （opp content）。2 人影，物影。3 格式；表格紙 （＝ 〈美...
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  1. The most common form of dna profile, abbreviated rflp, is a way of showing the unique patterns of bases in some of these areas

    Dna剖析圖測定最普通的一種形態，縮寫為rflp ，是用來表示這樣一些區域中堿基的特有圖案的一種方法。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  4. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  5. The large numbers of hydrogen bonds that form between the base pairs along a dna or rna molecule stabilize the double helix

    這些互補堿基對之間形成的大量的氫鍵可以維持雙螺旋結構的穩定。
  6. In the form of a double helix, dna contains the genetic blueprint of an organism

    脫氧核糖核酸脫氧核糖核酸外形呈雙螺旋鏈狀，內里記錄了遺傳訊息的編碼。
  7. They ' re a rich form of selenium, a trace mineral that convinces cancer cells to commit suicide and helps cells repair their dna

    這樣的沙拉富含硒（一種礦物質元素能夠殺死腫瘤細胞並幫助細胞修復其dna ） 。
  8. On the timescale that the unwanted bonds form, een a rapidly moing dna molecule would essentially appear frozen

    在不必要的鍵形成的標量時間中，即使快速移動的dna分子也必須要凝固。
  9. On the timescale that the unwanted bonds form, even a rapidly moving dna molecule would essentially appear frozen

    在不必要的鍵形成的標量時間中，即使快速移動的dna分子也必須要凝固。
  10. Dna, or deoxyribonucleic acid, is the substance ? specifically, a nucleic acid molecule with a twisted double strand double helix form ? that carries the genetic information for humans and other living organisms from one generation to the next

    Dna即脫氧核糖核酸，是一種雙螺旋鏈核酸分子，它攜帶人類及其他生物的遺傳信息，使其代代相傳。
  11. A complete round of transcription involves the recruitment of polymerase and general transcription factors to the promoter, rna chain synthesis initiation and polymerase escape form the promoter, rna chain elongation, and finally termination with relaease of polymerase and nascent transcript from the dna template

    一個完整的轉錄循環包括ranp和通用轉錄因子被招募至啟動子、 ran鏈合成的起始和ranp從啟動子的脫逸、 rna鏈的延伸以及伴隨ranp和新生rna鏈從dna模板釋放的轉錄終止等過程。
  12. Measurement of the fragment lengths was accomplished with the use of scion image analyzed software, which allows a curved fragment to be measured in a stepwise manner at nanometer level resolution, and the change laws of three forms of dna, supercoil, open circular and linear form as the dose increased were obtained

    藉助于afm ，對粒子和兩種重離子誘發的dna損傷進行了納米水平的結構分析，並結合scionimage圖像分析軟體的分段測量方法，對輻照誘發的dna碎片長度分佈進行了納米級分辨的測量。
  13. For more than half a century the fundamental story of living things has been a tale of the interplay between genes, in the form of dna, and proteins, which the genes encode and which do the donkey work of keeping living organisms living

    半個多世紀以來，關于生命的大部分故事說白了就是以dna和蛋白質形式的基因間相互作用的故事，這些基因經過編碼和簡單的組合就可以使有機體具有生命。
  14. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  15. It appears to require embodying a minimum of 15, 000 strands of dna to reverse all decay within a human form

    要在人體形態中逆轉所有衰亡，看起來需要包含至少15000股dna 。
  16. Regeneration beyond the first 12 strands of dna requires a new biochemistry that is known as the “ crystalline ” form

    超越最初12股dna后，則需要一個新的被知曉為「水晶」形體的生化體。
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