acceptor gene 中文意思是什麼
acceptor gene
解釋
受體基因-
Because of the special biological structure of bryology, it was very difficult to transfer foreign gene into the protonema or gametophyte by agrobacterium - mediated transformation. protoplasts as acceptor, using direct dna transfer methods such as microprojectile bombardment and peg - mediated transformation is becoming a good way
由於蘚類植物特殊的生物學結構用農桿菌侵染其原絲體或者莖葉體很難實現轉化，以原生質體作受體是蘚類植物轉化的常用途徑。 -
The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。 -
It is possible that exogenous dna fragment integerated into acceptor genome, changing gene base sequence or base deletion or base insertion, inducing to mutant at gene level
這可能是外源dna片段整合進受體的基因組中，改變基因的順序或者引起基因堿基的缺失、插入，從而在基因水平上發生突變。 -
Studies on transformation of indica rice with bt - toxin gene mediated by agrobacterium tumefaciens precultured immature embryo and callus derived from young panicle, immature embryo and mature embryo were used as acceptor for genetic transformation mediated by agrobacterium tumefaciens, the transformation rate of the above acceptor was investigated respectively. the results showed that immature embryo after precultured for 4 ~ 6d was the best. in respect to the concentration of agrobacterium tumefaciens when calli were cotransformated in medium yeb, to agrobacterium tumefaciens eha 105, od value of 0. 8 was the best
採用農桿菌介導法將bt毒蛋白基因導入水稻同樣以上述兩種秈稻為主要研究材料，比較了分別以預培養的幼胚和幼穗、幼胚、成熟胚來源的愈傷組織作為轉化受體的愈傷組織轉化頻率，結果表明預培養4 6天的幼胚最適宜作為農桿菌介導轉化的受體；其次是來源於幼胚和成熟胚的生長狀態良好的胚性愈傷組織。
分享友人