acci 中文意思是什麼
acci
解釋
阿奇-
In this study, a 1. 7kb kpni fragment and a lacz gene expression cassette carrying the e. coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ). the new transfer vector was called puni - lacz. the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的經典弱毒疫苗bartha - k61株為親本株，在通用prv轉移載體pbdtk - uni的基礎上，在其多克隆位點中插入由sv40早期啟動子控制下的lacz基因表達盒，同時將下游同源臂增加了一個1 . 7kb的kpni片段，使上下游同源臂的長度都超過了1kb ，構建了一個新的轉移載體puni - lacz 。 -
And then a 378bp acci fragment in pbtk2. 6 was replaced by a fragment containing the immediate early promoter of cytomegalovirus and bovine growth hormone polyadenylation signal derived from pcr3 - uni, a eukaryotic expression plasmid
在此基礎上，進一步用tthlll酶切后，補平插入帶有sv40啟動子的大腸桿菌?半乳糖苷酶報一告基因（ lacz ） ，獲得通用轉移載體pltk - uni 。
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