activity vector 中文意思是什麼
activity vector
解釋
活動向量-
Rlean meat percentage is one of the most important economic traints in pig breeding programs. myostatin is a negative regulator of skeletal muscle growth. null or low activity of myostatin, individual muscle of mutant amimals would show a large and widespread increase in skeletal mass. myostatin null animals have significantly larger diameter or more quantity of fiber skeletal muscle. the phenotype was termed double muscling. in order to probe the relation between myostatin and high lean meat rate and plump - hipped trait, we sythesized the c ' 80 amino acids coding sequence of porcine myostatin and costructed the cloning and expressing vector of it
肌生成抑制素（ myostatin ，即mstn ）是近幾年來（ mcpherrona . c等， 1997 ）發現的骨骼肌生長的負調控因子，它主要在骨骼肌中表達。其活性的喪失，會引起動物肌肉的過度發育，肌纖維直徑變大或肌纖維數增加，表現為雙肌癥狀。肌生成抑制素研究的突破將對豬、肉雞、肉牛等畜禽生產性能的提高具有特別重要的意義。 -
To test the p7 promoter activity, a series of constructs were obtained by cloning the different dna fragments into the luciferase gene reporter vector pgl3 - b. when the constructs were transiently transfected into thp - 1 cells, luciferase activity assay showed that the core region of p7 promoter located at - - 165 bp
第一部分的工作首先通過對人acat - 1基因p7啟動子序列進行5 '和3 ' -端的缺失的詳細分析，結果顯示最大報告基因轉錄表達活性位於- 612 - 165bp區段。 -
A set of microemulsions of water / span80 / transformer oil with different water content are prepared and their optical activity are measured with the changes of applied electric field and, the angle between the electric vector of the incident linearly polarized light and the external electric field, using an automatic polarimeter
對于某一濃度的微乳液，在外電場的作用下，旋光角平隨0變化且先增大后減小。實驗測得微乳液的電致旋光效應為正，即外電場作用下，微乳液樣品為右旋物質。 -
The native expressed product from e. coli bl21 ( de3 ) strain, however, showed weak activity against tmv. gp609 and zwemu were inserted into pemu - mcs - n, an expression vector for monocotyledon. and rhxjb was inserted into pkylx71 : 35s2
將dna一8001連接到pet一sa表達載體上，在大腸桿菌blzi （ de3 ）菌株中實現天然表達，表達產物對tmv的抑制效果很差，其原因可能是c一末端延伸序列的存在抑制了抗tmv活性的發揮。 -
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。 -
Experiments are reduced by quantitative structure - activity relationship, then a lot of work, money and time is saved. camptothecin derivatives are a kind of prospective anticancer medication. experiments suggest support vector regression is
然後應用支持向量回歸方法預測了芳香烴化合物生物降解度與喜樹堿化合物抗腫瘤生物活性，得到了較好的結果。 -
Activity level vector
活動水平向量 -
These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5
方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ e -
4 we observed that t cell vaccine activity could be greatly improved through immune - sensibilization to mice with hcv adenovirus vectors or plasmid vector before t - cell vaccine induction, and adenovirus vectors proved to be superior to plasmid vector
在誘導t細胞疫苗之前，用v腺炳毒載體或質粒載體討小鼠進廳免疫致敏，可以明顯提高t細胞疫苗的活性；其中腺病毒載體的免疫致敏效果要明顯好於質粒載體。 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。 -
( 3 ) establishing a new method which can quantitatively determinate telomerase activity. 1. human telomerase rna component ( htr ) gene was cloned from hela cells using gene engineering technology. the htr gene was then reverse inverted into retrovirus vector plncx and constructed an antisense recombinant plasmid plncx - atr
將htr基因插入逆轉錄病毒載體plncx中，構建了htr基因的反義重組質粒plncx - atr和正義重組質粒plncx - tr ，測序結果表明符合預期要求，為下一步實驗奠定了基礎。 -
To improve the production and activity of the enzyme, the hydantoin hydrolase gene ( hyuh ) was cloned into e. coli m15 and bl21 ( de3 ) by using vector pqe60 and pt221 respectively. two expression plasmids were thus constructed
用pcr技術從質粒puc18 - 169中擴增出l -乙內酰脲水解酶基因（ hyuh ）和n -氨甲酰基氨基酸水解酶基因（ hyuc ） ，分別在大腸桿菌和畢赤酵母中實現了活性表達。 -
Multiple kinase assay was performed to examine pkc ^ mapk. tpk activity in the transfected cells. meantime, pegfp - sh2a vector was also constructed and the cells transfected with it were examined by fluorescent microscopy. the expression of sh2a gene was examined under different concentration and time of bfgf as a stimulating factor
1 sh ，利用脂質體轉染肝癌bel7402細胞人os7細胞，檢測pkc 、 mapk 、 tpk活性的改變；流式細胞儀檢測細胞增殖；另構建pegfp sh ，轉染細胞，熒光顯微鏡觀察熒光定位； bfgf作為刺激因素處理細胞，根據不同濃度、時間檢測sh基因表達情況。 -
Now, many machine learning methods, such as artificial neural network and multivariate regression analysis, are applied to quantitative structure - activity relationship. in this paper, support vector regression is applied to quantitative structure - activity relationship
本文在簡介定量構效關系發展狀況的基礎上，介紹了幾種定量構效關系的解決方法，其中重點介紹了多元線性回歸方法和人工神經網路方法。 -
Klac gene was ligated to the pet - 30a ( + ) vector for expression. then the recombinant plasmid petlac4 was transformed into e. coli bl21. the positive transformants were induced at different temperature for three hours by iptg with the final concentration of lmm. sds - page analysis and lactase activity assay showed that klac gene was expressed in e. coli, and that the expression level at 28 was much higher than that at 37
表達產物的sds - page分析和酶活性測定表明， klac基因在大腸桿菌中獲得活性表達，其中低溫條件28下的表達水平明顯高於37 ， 28誘導的細胞經超聲波破碎所測酶活力為0 . 475u / ml ， 37僅為0 . 134u / ml 。 -
Objective to prepare ea and ed protein of p - galactosidase with gst fusion protein by pgex - 4t - 2 expression system, and study its a complementation activity. to lay a good foundation for further study of cedia and utilization in expression immunoassay. methods an artificial synthesized dna segment coding for residues 6 - 56 ( modified ) of p - galactosidase ( ed protein ) was ligated to pgex - 4t - 2 vector
實驗方法通過人工合成編碼d半乳糖昔酶ed蛋白的dna並插入原核表達載體pgex 4t 2 ；從陀v p galactosldasecontrolvector質粒中用sal及earl進行雙酶切得到編碼卜半乳糖昔酶a一受體（ ea ）的大部分dna ， n端再加上約60hp人工合成洲a ，連接后轉入pgex葉t億載體中。
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