acutus 中文意思是什麼

acutus 解釋
急尖形
  1. The 600 bp and 800 bp pcr products were cloned into the pgem - t easy vector. their cdna sequences were determined with positive clones or purified pcr product. conclusion : compared the 600 bp pcr product with the amino acids sequence for the fibrinolysin metalloproteinase from the venom of agkistrodon acutus from the southern of anhui province, their homology is 90. 6 %

    結果：其中一對引物擴增得到一600bp產物；另一對引物擴增得到三條特異性的dna條帶，大小分別為1 . 5kb 、 1 . 3kb和800bp ，將600bp和800bpdna進行克隆及測序，並推導編碼的氨基酸序列。
  2. The pictured critter was not even the elusive american crocodile, crocodylus acutus

    圖上的動物甚至不是神出鬼沒的美洲鱷。
  3. It is shown that the obtained proteins ( hq - 5 & tsk - 2 ) can be identified as novel components of anti - coagulation enzyme family in the venom of agkistrodon acutus by the analysis of the n - terminal sequence of tsk - 2

    其一級序列為rtefqrymeivv 。與從蘄蛇蛇毒中分離出的其它組分相比， hq - 5及其降解產生的組分（ tsk - 2 ）為新的抗凝血組分。
  4. Objective : to clone and sequence the cdna encoding metalloproteinase from the venom of agkistrodon acutus from northen mountain area of guangxi province. methods : one step method was used to extract total rna from the venom of agkistrodon acutus found in northern mountain area of guangxi province. different kinds of cdna encoding metalloproteinase were amplified by one step method ( rt - pcr and pcr reactions occurred in the same tube ) using different primers

    方法：從桂北五步蛇毒腺中抽提總rna ，利用不同的引物，採用一步法（ rt - pcr和pcr在同一管內進行）擴增出不同的dna條帶，利用平端連接的方法將pcr擴增產物克隆至pgem - teasy載體，轉化大腸桿菌jm109 ，挑選白色菌落提取質粒，用pcr對其進行鑒定，直接利用純化pcr產物或提取陽性菌落質粒進行測序。
  5. The cdna sequence with 789 bp encodes an uncompleted open reading frame ( orf ) of 263 amino acids, which included a signal peptide of 18 amino acids, a proposed propeptide of 171 amino acids, a partial mature protein of 74 amino acids. the sequences of signal peptide and propeptide are very similar to ones from wannan agkistrodon acutus reported by fan chunyan, but it only contains partial amino acids of mature protein. the 800 bp is likely to be a part of gene encoding metalloproteinase agkistrodon acutus from guangxi province

    結論： 600bp產物和已報道的皖南五步蛇纖溶酶金屬蛋白酶氨基酸序列相比較，有90 . 6 %的同源性； 800bp產物中信號肽及前肽和已報道的皖南五步蛇金屬蛋白酶很相似，同源性為97 . 9 % ，但僅含有部分金屬蛋白酶成熟肽的氨基酸序列，推測此片段為廣< wp = 6 >西五步蛇金屬蛋白酶原基因的一部分。
  6. Method was investigated to extract multiple proteins from complex system including water - soluble fraction of egg yolk and agkistrodon acutus venom by bacterial specific absorption. immunoglobulin yolk ( igy ) were absorbed directly from water - soluble fraction by streptococcus mutans, then eluted and further purified by columns of thiophilic gel. based on above experiments of antibody extraction, bacterium cells were applied further to extract interactive proteins from crude venom of agkistrodon acutus

    首先，利用變異鏈球菌（ s . mutans ）與其特異性抗體的吸附作用從水溶性蛋白組分中分離出卵黃抗體（ igy ） ，並將菌體上洗脫下來的igy用嗜硫色譜分離純化，得到高純度的細菌特異性抗體。
分享友人
分享到 Line

分享到臉書