affinity column 中文意思是什麼
affinity column
解釋
親和層析柱-
The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography. the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b. after washing the unspecific proteins, change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively
方法是先用硫酸綏鹽析，再過親和層析柱，即33飽和硫酸按沉澱后，透祈過夜，上p24抗原做配體的濱化氰活化的sepharose4b柱，流洗掉雜蛋白和抗人紅細胞單特異性抗體后，再洗脫並收集雙特異性抗體。 -
In this study, a novel strategy to inhibit the activation of the complement system has been developed. to get the purified clq as the target molecule for biopanning, the first step of our purification was to isolate the clq from clr2cls2 by affinity chromatography on igg - sepharose 4b column
本研究另闢蹊徑，以補體經典激活途徑始動分子c1q為靶標，採用噬菌體肽庫技術，親和篩選能與c1q結合的噬菌體克隆，研製可抑制補體激活的c1q模擬短肽。 -
Were passed through affinity column, separated by sds - page and stained using con a - hrp
用cona - hrp檢測獲得分子量為37和39kd兩種糖蛋白特異成分。 -
A sepharose - heparin column ( a affinity chromatoraphic column ) was used to study the binding of heparin to protein previously. in the 1990 ' s, a sensitive technique developed for the study of the potential interaction of cytokine with heparin and related polysaccharides is affinity coelectrophoresis ( ace )
以往主要是通過sepharose - heparin親和層析來研究肝素與蛋白的相互作用； 20世紀90年代又發展了affinity - coelectrophoresis （ ace ）技術研究蛋白質與肝素的相互作用。
分享友人