affinity purification 中文意思是什麼

affinity purification 解釋
親和沉澱
  • affinity : n 1 姻親關系 〈cf consanguinity〉; 密切關系。2 （語言等的）類似，近似。3 （男女之間的）吸引力，吸...
  • purification : n. 1. 清洗，洗凈；凈化（作用）。2. 提純，精製。3. 【宗教】滌罪；潔身；齋戒；潔禮，祓；【天主教】潔杯式。
  1. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  2. Purification and renaturation of recombinant human cu, zn - sod by metal - chelating affinity chromatography

    金屬螯合親和層析純化和復性重組人銅鋅超氧化物歧化酶
  3. In this study, a novel strategy to inhibit the activation of the complement system has been developed. to get the purified clq as the target molecule for biopanning, the first step of our purification was to isolate the clq from clr2cls2 by affinity chromatography on igg - sepharose 4b column

    本研究另闢蹊徑，以補體經典激活途徑始動分子c1q為靶標，採用噬菌體肽庫技術，親和篩選能與c1q結合的噬菌體克隆，研製可抑制補體激活的c1q模擬短肽。
  4. After purification using glutathione sepharose 4b affinity chromatography and digestion with thrombin, the recombinant ntfs were found to be biologically active in the pc 12 cells neurite outgrowth assay. the assays demonstrated that the purified ntfs proteins exhibited normal activity, which is the first step in developing a comprehensive gene therapy for nerve diseases of the giant panda and the crested ibis

    最後，對重組表達蛋白經glutathlonesepharose4bmicrospincolumn親和純化后，進行大鼠腎上腺嗜鉻瘤細胞（ p2 ）神經營養因子的活性鑒定，發現其能夠誘導神經細胞分化產生突觸，即具有預期的生物學活性，表明所獲表達蛋白為大熊貓nt
  5. After the gst fusion proteins were processed by factor xa and were purified by gluta - thione - sepharose 4b affinity chromatography, the recombinant protein hbrp with high purification would be acquired. during the process of separation and purification, the every result which can show the location of the aim protein was monitored by 10 % sds - page. the gst fusion proteins acquired finally were examined and analyzed by the methods of western - blottin

    原核表達系統具有表達效率高，操作方便等特點，但缺乏翻譯? 3 ?后修飾，以pgex載體表達的gst hbrp融合蛋白，具有表達的蛋白穩定性好，在體外易於純化等優點，表達的gst融合蛋白可用gluthioneseph刪sc4b進一步純化一factorxa酶切與測分離，從而大量獲得hbrp重組蛋白，而且一次回收率大約在90以上。
  6. The purification and effects on reproduction of bull sperm - surface glycoprotein having affinity for con a

    一種牛精子膜蛋白的純化及在生殖中的作用
  7. After that a good renature result was obteined using gradient with lower protein concentration. the purity of the recombinant protein by affinity - purification of glutathione sepharose 4b and electrodialysis method was also attained perfect result. the antigenicity of the gst - ha1 protein were identified using elisa mathod

    用梯度透析法對變性的融合蛋白gst - ha1進行復性，在低蛋白濃度的條件下，獲得了很好的復性結果，復性前蛋白濃度為1 . 02mg ml ，復性后蛋白濃度為0 . 875mg ml 。
  8. The yeast recombinant expression vector pplc3. 5k - ndrg2 was constructed, then the ndrg2 protein was expressed in soluable form in pichia. after purification by metal chelate affinity chromatography, we get 6his - ndrg2 fusion which be nature in structure and will be useful to reseach its functions in future

    為研究ndrgz的結構與功能，構建了ndrgz的酵母融合表達質粒，並在比赤酵母中得到可溶形式的表達；利用金屬螫合親合層析純化出了6his融合的ndrgz蛋白。
  9. After dissolving inclusion bodies, renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ), the fusion protein trx - scfv of electrophoretic purity was obtained, they still possessed antigen - binding activity

    經變性復性，用固相金屬離子親和色譜法一步分離純化了具有功能的單鏈抗體，在一升的發酵液中得到78mg的重組蛋白。
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