agarose gel 中文意思是什麼

agarose gel 解釋
瓊脂糖膠
  • agarose : 瓊膠糖
  • gel : n. 凍膠，凝膠（體）；定型發膠。vi. （-ll-） 成凍膠，膠化。
  1. In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization

    本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。
  2. The result of the agarose gel electrophoresis showed that the length of the full - length cdnas in the library was pooled mainly between 500 and 2 000 base pairs

    結果表明獲得的ejoi基因的cdna長度為876hp ，開放閱讀框長度為759hp ，編碼252個氨基酸。
  3. The pcr products were electrophoresed on 3 % agarose gel and visualized under uv light

    聚合酶鏈鎖反應的產物以3 %瓊脂電泳分離並在紫外光下觀察結果。
  4. Materials and methods in our study, first the e. coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis. the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i. the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis

    同預想結果基本吻合，大規模培養后純化得到的融合蛋白通過sds page顯示在52kda處有一條帶，而酶切后產物電泳結果顯示在26kda處分另有兩條蛋白帶，其中一條為gst ，另一條為hbrp ，基本符合實驗結果； hbrp對tpk活性的抑制呈劑量依賴性。
  5. Apoptotic peak. agarose gel electrophoresis showed that 185 - base pair ladder characteristic of the dna degradation that occurs in apoptotic cells induced by a

    電泳出現階梯狀條帶流式細胞儀檢測出現典型的凋亡峰，提示
  6. The apoptosis induced by extract of russula subnigricans hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis. the result showed that agarose gel electrophoresis of dna extracted from poisoned little white rat liver and kidney cells revealed typical 180 ~ 200bp integer - fold " ladder " " bands. apoptosis induced by extract of russula subnigricans hongo was dose - and time - dependentthe result indicated that extract of russula subnigricans hongo could induce apoptosis in little white rat liver and kidney cells

    4 .用電泳技術研究亞稀褶黑菇粗毒液誘導小白鼠肝腎細胞凋亡，小白鼠亞稀褶黑菇抽提液中毒后，肝腎細胞. dna經瓊脂糖凝膠電泳出現180一200bp整數倍的ona梯形帶， 19 . 09 / l一28 . 59 / l范圍內，亞稀褶黑菇提取液誘導肝腎細胞凋亡表現出時間和劑量依賴性
  7. The biological characteristics and toxicity of russula subnigricans hongo was studied for the first time from ecology and morphologic characteristics and histology, the orthogonal experiment of the optimum culture condition, the analysis of components, apoptosis of the cells from little white rat liver and kidney induced by extract of russula subnigricans hongo, to the histopathologic changes observation of little white rat liver and kidney through ecological observation, light microscopy and scanning electron microscopy, reversed - phase high performance liquid chromatography, agarose gel electrophoresis, transmission electron micioscopy. the result showed as below : based on ecological observation of russula subnigricans hongo, its ecological environment was investigated in order to simulate its ecological environment when they are cultivated

    利用菌種分離技術、光鏡技術、電鏡技術、高效液相色譜技術、毒理實驗技術、電泳方法等對亞稀褶黑菇（ russulasubnigricanshongo ）的生物學特性和毒性機理進行了研究，主要包括以下內容：亞稀褶黑菇的生態學和組織學研究、菌種分離培養、掃描電鏡觀察、成分分析、粗毒液誘導小自鼠肝腎細胞凋亡，小白鼠中毒后肝腎細胞透射電鏡觀察，研究結果如下： 1
  8. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  9. I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

    從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。
  10. Dna ladder bands with a periodicity of about 200 bp were clearly seen in agarose gel electrophoresis pattern

    瓊脂糖凝膠電泳基因組dna可見到約以200bp為間隔的dna梯狀（ ladder ）條帶。
  11. Methods : the effects of different neurotrophic factors on the growth and differentiation of neural stem cells were observed by cells counting and immunofluorescence staining. the levels of rara mrna and rxra mrna in differentiated neural stem cells were assayed by rt - pcr. agarose gel electrophoresis and image analysis

    方法應用細胞計數和免疫熒光細胞化學法，研究不同神經營養因子對神經幹細胞增殖及分化的影響；應用rt - pcr 、瓊脂糖凝膠電泳和紫外分光圖象分析法檢測神經幹細胞分化過程中rar和rxr mrna表達量的改變。結果1
  12. In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken

    為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。
  13. It has marked apoptotic induction on mouse spleen cells tested by dna agarose gel electrophoresis analysis and flow cytometric assay in certain degree. it also can decrease the activity of lactate dehydrogenase ( ldh ) outside of mouse the spleen cells and increase the quantity of reduced gsh inside the mouse spleen cells

    而且一定濃度范圍內可引起小鼠脾淋巴細胞dna發生特徵性降解，出現dnaladders ，引起染毒組細胞凋亡率顯著性升高，但細胞凋亡率與劑量不呈直線相關關系。
  14. A final period of extension was carried out for 9 min and final holding at 4. the pcr products were resolved after electrophoresis in 2 % agarose gel with ethidium bromide. the pcr products were visualized while the gel was exposed to ultraviolet light

    擴增產物在2瓊脂糖凝膠上電泳，用pcrmarker作分子量標準，紫外燈下觀察，最適pcr反應條件為擴增出最強的sry產物帶而無非特異性擴增帶出現時的反應條件。
  15. 4 ) fragement recovery of pcr products pcr products were separated on a 1. 5 % agarose gel. the expected fragment was recovered by using pcr fragment recovery kit according to the manufacturers instructions

    4 、凝膠回收應用pcr目的基因片段回收試劑盒對擴增陽性pcr產物進行凝膠回收，純化目的dna片段。
  16. To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis

    本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
  17. The high purity of genomic dna extracted by tripure isolation reagent was observed. dna agarose gel electrophoresis showed that the genome had a high integrity without degradation. and also, spectrophotometric analysis indicated that the genomic dna had no pollution by protein and rna. 2

    培養乳酸乳球菌nizor5 ，收集菌體，用tripurelsolationreagent提取其基因組dna ，瓊脂糖凝膠電泳，紫外顯示儀下可見在點樣孔附近有一條整齊均一的dna帶。
  18. A dna band about 1700 bp in length was found in the rt - pcr products of both strains after an agarose - gel electrophoresis, which was consistent with the length of hn registered in genbank

    瓊脂糖電泳結果顯示，其大小均約為1700bp ，與genbank登錄的大小一致。其次，將hn基因cdna定向插入pcdna3中。
  19. Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method

    首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。
  20. Agarose gel electrophoresis video clip wmv format, window media player required

    瓊脂糖凝膠電泳示範片段wmv格式，需用window media player播放
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