agrobacterium tumefaciens 中文意思是什麼

agrobacterium tumefaciens 解釋
根癌病
  1. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  2. A new transgenic system of gerbera mediated by agrobacterium tumefaciens

    小細胞團為受體的根癌農桿菌介導的非洲菊基因轉化體系
  3. As explants, were infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring the expression vectors pbgsb and pbglsb respectively and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 1 mg / l 6 - ba + o. l mg / l naa + 0. 7 % agor ph 5. 7 )

    Ling inaa ）中暗培養三天後，移入加入抗生素100mg lkan和500mg幾cr的誘芽培養基，于組培室中進行誘芽和抗性篩選。
  4. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草（品種sr ）無菌苗葉片為外植體，採用農桿菌浸染的葉盤轉化法，用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基（ ms 0
  5. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。
  6. In the study of floral - dip method, flowering inflorescences of oilseed rape growing in field are immersed in floral - dip solution resuspended with cultured agrobacterium tumefaciens lba4404 or eha105, resided binary plasmid pbi121

    在油菜花序浸泡法（ noraldip ）轉基因的研究中，用含雙元載體及報告基因的農桿菌在生產大田直接浸染正在開放的油菜花朵。
  7. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  8. Effects of antibiotics on inhibition of agrobacterium tumefaciens and differentiation of tobacco leaf

    抗生素對根癌農桿菌的抑菌效果及對煙草葉片分化的影響
  9. In the present study, an omanental flower sinningia speciosa was transformed with tmek2 and tmek2mut :, the wild type and mutant type of mapkk from tomato, by agrobacterium tumefaciens

    本研究用野生型、突變型mapkk基因轉化觀賞花卉大巖桐，為進一步研究mapkk基因的功能奠定基礎，也為花卉植物的轉基因提供實驗系統。
  10. A binary plant expression vector with osg6b " driving report gene gus was constructed and transferred into tobacco via agrobacterium tumefaciens mediation

    將克隆的啟動子與報告基因gus相連，構建植物表達載體，通過農桿菌介導轉化煙草。
  11. The modified sequence was cloned into binary vector pbi121. the constructed expression vector was named pbi121 - bar, then transferred into agrobacterium tumefaciens lba4404 by triparental crossing. thus the project bacterium has been successfully constructed

    改造后的目的基因克隆于真核表達載體pbi121 ，構建表達載體pbi121 - bar ，通過三親雜交法將真核表達載體pbi121 - bar轉入農桿菌lba4404 ，成功構建出工程菌。
  12. Tissue culture of soybean ( glycine max l ) and stem mustard ( brassica juncea mao ) in vitro and genetic transformation of soybean by agrobacterium tumefaciens were studied in this experiment

    本論文研究了大豆的離體再生和根癌農桿菌介導的遺傳轉化及榨菜的離體培養。
  13. The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin

    本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
  14. In this research project, the agrobacterium tumefaciens mediated transformation of badh gene of tetraploid black locust has been studied, for the purpose of improving resistance to soline - alkali and drought of tetraploid black locust ; of playing more important role in developing of waste lands, ameliorating of soline - alkali soil, and greening and beautifying of surface - mined lands, mine waste dumps, slopes of roads and railroads where restoration of vegetative cover has proven difficult ; of fully making benefits of its ability to fix atmospheric nitrogen in the soil

    為了進一步提高四倍體刺槐的耐鹽性和抗旱性，進一步擴大其適宜種植的生態范圍，充分發揮其固氮、改良土壤的特性，在我國的城鎮綠化、荒山造林、鹽堿地改良以及采礦跡地、公路、鐵路邊坡等植物生長困難土地的植被恢復中發揮其優勢，本實驗對四倍體刺槐進行了農桿菌介導的甜菜堿醛脫氫酶基因轉化的研究。
  15. Then, we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens. after verified by antibiotic resistance, reporter gene examination, southern blot detection, and genetic segregation analysis, we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene, respectively. further, we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants

    對得到的再生煙草植株分別進行了報告基因表達水平檢測、 southern - blot鑒定以及t _ 0代轉基因種子遺傳分離規律分析，分別得到了單拷貝的穩定表達番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的轉基因煙草3株、 7株和1株，同時還得到單拷貝的只轉化gusa基因的陰性對照轉基因煙草3株。
  16. The high performance two - gene expression vector pc3c ! c2 was transformed to nicotiana tobacum honghuadajinyuan plants mediated by agrobacterium tumefaciens eh a105 according to the leaf disc procedure. transformed shoots were selected on solidified medium containing l00mg / l kanamycin

    將含有phac1和phac2雙基因的植物高效表達載體pc3c1c2 ，用凍融法，構建了pc3c1c2根癌土壤桿菌（ agrobacteriumtumefacienseha105 ）的工程菌。
  17. In this construct, hf2 dna should be expressed under the control of the camv 35s promoter. the construct was transformed to petunia hybrida of the light pink via agrobacterium tumefaciens eh a105 using the leaf disc method. in the end, three transgenetic plants were obtained by screening with the phosphinothricin resistance and pcr amplification

    通過三親交配將重組質粒pc3301 - hf2導入根癌農桿菌eha105 ，抗性篩選、 pcr檢測及dna點雜交表明轉化后的農桿菌帶有完整目的基因片段，能夠用於轉化植物。
  18. ( 3 ) the optimal explant age for transformation was 6 or 7 day after aseptic germination of seeds. ( 4 ) development of non - transformated cell was inhibited completely by 100mg / l kan. ( 5 ) 500mg / l cb could avoid pollution of agrobacterium tumefaciens and had no distinct effects on the formation of callus and shoot regeneration. ( 6 ) the optimal co - cultural condition was 3 day on 28 in dark

    以胡蘿卜品種「改良黑田」為受體材料，以胡蘿卜幼苗的下胚軸為外植體，建立了農桿菌介導法的胡蘿卜轉化體系，並對其中的種子消毒、激素水平、外植體的苗齡、選擇壓、共培養時間等關鍵步驟的條件進行了優化。
  19. Studies on transformation of indica rice with bt - toxin gene mediated by agrobacterium tumefaciens precultured immature embryo and callus derived from young panicle, immature embryo and mature embryo were used as acceptor for genetic transformation mediated by agrobacterium tumefaciens, the transformation rate of the above acceptor was investigated respectively. the results showed that immature embryo after precultured for 4 ~ 6d was the best. in respect to the concentration of agrobacterium tumefaciens when calli were cotransformated in medium yeb, to agrobacterium tumefaciens eha 105, od value of 0. 8 was the best

    採用農桿菌介導法將bt毒蛋白基因導入水稻同樣以上述兩種秈稻為主要研究材料，比較了分別以預培養的幼胚和幼穗、幼胚、成熟胚來源的愈傷組織作為轉化受體的愈傷組織轉化頻率，結果表明預培養4 6天的幼胚最適宜作為農桿菌介導轉化的受體；其次是來源於幼胚和成熟胚的生長狀態良好的胚性愈傷組織。
  20. In this paper, we transferred ced - 9 gene, an anti - apoptosis gene, into tobacco and rice by agrobacterium tumefaciens eha105. the results are showed as follows : 1

    Ced - 9是發育pcd的負調控基因，本課題將ced - 9基因通過根癌農桿菌（ agrobacteriumtumefaciens ） eha105介導轉化煙草和水稻，獲得以下結果： 1
分享友人
分享到 Line

分享到臉書