aminosilane 中文意思是什麼
aminosilane
解釋
氨基硅烷-
The results of the experiments indicates that concentration of aminosilane influences the fluorescence background of glass slide, and some factors affect immobile ratio of oligonucleotide probe, such as aminosilane treatment time, aldehyde treatment time, uv crosslinking energy, washing temperature and time
研究表明，氨基化試劑濃度對玻片熒光背景有影響，氨基化試劑處理時間、醛基化處理時間、紫外交聯能量和洗滌溫度和時間等工藝因素影響寡核苷酸探針的固定率。 -
The key stage of fabricating gene chip is pretreatment of glass surface including the processes of nh3h2o treatment, aminosilane treatment and aldehyde treatment. the pretreatment can grow active group that can bind probe effectively on the surface of glass slide. as a result, the actively treated glass slide can suit for fabricating in - situ synthesis high density gene chips
基因晶元制備技術的關鍵步驟是玻片表面預處理，即對玻片表面進行羥基化、氨基化和醛基化處理，使表面生長的活性基團能有效固定寡核苷酸探針，以滿足原位合成高密度基因晶元對玻片的要求。 -
First, glass slides having been rinsed will be treated with nh3h2o, aminosilane and aldehyde. second, the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope. in the end, the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods
實驗選用表面平整的德國玻片，將清洗好的玻片分別進行羥基化、氨基化、醛基化，採用熒光法和原子力顯微鏡法分別檢測玻片表面預處理質量，研究兩種檢測方法之間的內在聯系，從而確定表徵玻片表面寡核苷酸探針固定率的方法。 -
Aminosilane coupling agent
氨基硅烷偶合劑 -
The result of fluorescence show that the fluorescence intensity of the surface of the treated glass slide connect with the probe immobile ratio of oligonucleotide. the more oligonucleotide probes have been linked with active group, the stronger fluorescence intensity is. for the strongest fluorescence, the technical conditions is : treatment of 2 % aminosilane of 20 minutes, treatment of 5 % aldehyde of 24 minutes, uv crosslinking of 150mj and washing of 5 minutes at 20
兩種檢測方法表明，當活性基團呈柱狀、分佈均勻且尺寸比較大（ 200nm ）時，有利於寡核苷酸探針的連接，且連接探針數量多，玻片表面熒光強度強，固定率高；當活性基團呈錐狀、分佈及尺寸不均勻（ 150nm （ 300nm ）時，連接的寡核苷酸探針數量少，玻片表面熒光強度弱，固定率低。
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