amplicon 中文意思是什麼
amplicon
解釋
擴增子-
Result a total of five primer pairs was designed to cover the whole mtdna control region and the neighbor part. the length of amplicon was from 299 bp to 452bp with different primer pairs. the successful result was obtained even if the dna template was small to 0. 015ng
結果設計了覆蓋整個mtdna控制區及周圍區域的5對引物，使各段擴增產物長度在299bp到452bp之間，統一了擴增條件使5段序列可以在相同循環參數下擴增。 -
Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced
首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
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