amv 中文意思是什麼
amv
解釋
鳥類成髓細胞白血病簿-
Firstly, the major ampullate glands were got from araneus ventricousus. total rna and mrna of ampullate glands were isolated and purified. double strands cdnas were synthesized in the assistance of amv - rt and e. coli dna polymerase i etc. by reverse transcription and replacement synthesis
首先剝離大腹園蛛主壺腹腺，提取總rna和分離純化mrna ，反轉錄合成cdna ，凝膠層析除去小於400bp片段，並在雙鏈cdna兩端引入ecor的銜接頭，對其進行磷酸化處理。 -
Comparing with using mulv reverse transcriptase or amv reverse transcriptase to do reverse transcription at 3 7 - 42 " c, more accurate and complete dna could be gotten by using tag dna polymerase at 72 c since dimeric structure of mrna was opened fully at high temperature
與在37 - 42用mulv或amv進行反轉錄相比，在較高溫度下（ 72 ）用taqdna聚合酶進行反轉錄有利於正確、完整的cdna ，因為在高溫下mrna的二級結構被充分打開。
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