angiostatin 中文意思是什麼

angiostatin 解釋
血管生長抑素
  1. After that we determined the presence of angiostatin gene in the putative recombinant virus with pcr analysis

    之後利用pcr分析是否獲得重組angiostatin的桿狀病毒。
  2. 2d numerical simulation of effect of antiangiogenic factors angiostatin and endostatin on tumor - induced angiogenesis

    作用下腫瘤血管生成的二維數值模擬
  3. We also found an angiostatin - like protein and the detection of its activity on chick embryo chorioallantoic membrane model ( cam ) shows a sign of inhibition of the positive effect of lung cancer cell culture supernatant on cam. conclusion : development of proteomics brings out highly efficient approach for apr research, which overcomes the drawbacks of traditional methods including low efficiency and difficulty in detecting new apr - related proteins and the intrinsic false positive reaction of recently developed ssh technology

    結論：蛋白質組學為apr領域帶來高效率高通量的研究方法，改變了傳統的elisa方法需要事先確定靶蛋白的低效的，而且難以發現新蛋白的狀況，也克服了suppressionsubtractivehybridizaition （ ssh ）技術固有的假陽性，實現了對app候選蛋白快速的鑒定。
  4. Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully. recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins. the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2

    實驗結果我們成功地構建了滴度高達2x10 『 pm llil的angiostatin重組? 2 ?桿狀病毒，並在昆蟲細胞sffi中高效表達了分子量為53kd的an giostatin重組蛋白，重組angiostatin蛋白不僅在體外顯著抑制內皮細胞的生長， k 。
  5. In vitro endothelial proliferation inhibiting activity of recombinant angiostatin was examined with mtt method by using human umbilical vein endothelial cells ( huvec ). in this test we could draw the inhibition curve and calculated the ic50 to confirm its bio - activity further we do cam vascular inhibition test in vivo

    4驗證重組蛋白angiostatin的生物活性：採用mm法測定重組蛋白對原代培養的人臍靜脈內皮細胞（ huvec ）的抑制作用，繪出抑制曲線，並計算出ic 。
  6. The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock

    構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。
  7. Angiostatin, a multiple kringle ( 1 - 4 ) - containing fragment of plasminogen, is an effective natural inhibitor of tumor angiogenesis, which is a potent inhibitor of angiogenesis in vivo, and selectively inhibits endothelial cell proliferation and migration in vitro

    血管抑素angiostatin是纖溶酶原的內源片段，包括四個kringle結構，是一種重要的內源性新生血管生成抑制劑。
  8. Once the recombinant virus has been i - dentified, we amplified the p - 1 stock to attain the large scale, high - titer viral stock in order to initiate expression studies. the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared. the time course for expression of recombinant protein was detected by sds - page and western blot, which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein

    表達重組蛋白angiostatin ：用制備好的帶有矗s標記的融合性angiostatin基因的高滴度重組桿狀病毒貯存液感染草地貪夜蛾sfg細胞，用sds page電泳和hsternblot對感染不同時間後分泌的重組蛋白做時間表達分析，依此確定最適的感染復數（ moi ）和感染時間，以達到重組蛋白表達水平最適化，而後大規模進行重組蛋白的表達， sds page用來分析重組蛋白， westernblot用來在蛋白表達水平低的情況下檢測表達的特異重組蛋白。
  9. We sequence the inserted gene fragment of the indentified recombinant clone. the result is : angiostatin gene orf ( open reading frame ) links with the orf in expression vector correctly. but the first base of the codon aaa coding for lys414 in plg kringle 4 domain mutates from a to g which leads lys change to glu

    隨后取通過上述鑒定的重組克隆菌，對重組子插入片段測序，結果為： as基因開放讀碼框與表達載體的讀碼框正確匹配相連，但在其kringle4區相當于編碼plg的lys ~ （ 414 ）密碼子aaa的第一位堿基由a突變為g ，導致相應的氨基酸殘基突變為glu 。
  10. First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a

    用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。
  11. Angiostatin ( as ) is a kind of polypeptide identified as a product of plasminogen cleavaged by protease. it blocks neovascularization by directly inhibiting endothelial cell proliferation and migration

    血管抑素（ angiostatin ， as ）是血漿纖溶酶原（ plasminogen ， plg ）經蛋白水解酶降解生成的多肽片段，是直接作用於血管內皮細胞的血管生成抑制劑。
  12. Angiostatin can inhibit the angiogenesis of tumor, the tumor growth and metastasis through preventing tumor ' s blood and nutrition from supplying

    其能通過抑制腫瘤新生血管的生成，切斷腫瘤血液和營養的供應，從而抑制腫瘤的生長和轉移。
  13. Within the family of angiogenesis inhibitors, angiostatin, an endothelial cell - specific inhibitor, has been demonstrated to be the most potent

    在血管生成抑制因子的家族中血管抑素（ angiostatin ）作為內皮細胞特異性的抑制劑已顯示出強大的作用。
  14. Discussions angiogenesis, an essential process for tumor growth, is regulated by endothelial proliferation factors and their inhibitors such as angiostatin

    討論血管生成對于腫瘤生長來說是一個必需的過程，受內皮細胞增殖刺激因子和抑制因子的共同調控。
  15. This paper reviews the current research situation of angiostatin, the relationship between angiostatin and angiogenesis of solid tumor and the respect of application in cancer anti - vascular therapy

    綜述了血管生成抑制素的研究現狀，分析了它與實體瘤血管生成的關系，及其在實體瘤抗血管生成治療中的應用前景。
  16. Angiostatin is a angiogenesis inhibiting factor derived from tumor, which can potently inhibit endothelial cell proliferation and migration and has an important application value in the treatment of solid tumor

    摘要血管生成抑制素是腫瘤衍生的血管生成抑制因子，能有效地抑制血管內皮細胞的增生和遷移，在實體瘤的治療中有重要的應用價值。
  17. In this manner, angiostatin inhibits the growth of primary and metastatic tumors in vivo. it is a promising drug for angiogenesis - dependent diseases especially in pre - clinical and clinical cancer trials

    As可以阻抑血管生成治療由病理性血管形成而導致的疾病，尤其是可通過抗腫瘤新生血管生成抑制腫瘤的生長與轉移，有重要的臨床應用價值。
  18. The expression activity reached the highest point at the 72nd hour in bmn cell ( 22 u / 2x ! 06cells ) and at 144th hour in larvae ( 159 u / ml ), respectively. elisa assay showed that expression product had angiostatin ' s immunoreactivity. western blotting assay also showed that product expressed in cultured cells was a 36 kd band, while product expressed in larvae of silkworms was two proteins, and the molecular weights were 37 kd and 42 kd, respectively

    表達產物活性在家蠶細胞中表達72小時達到最高值，經胞內表達產物作用后，血管內皮細胞的存活率僅為（ 28 . 0 3 . 0 ） ，表達量約為22u 2 10 ~ 6個細胞；在家蠶體內表達144小時活性達到最高值，內皮細胞的存活率僅為（ 6 . 4 0 . 5 ） ，表達量約159u ml 。
  19. In the present thesis, it is mainly to express the human angiostatin ( kl - 3 ) in bombyx mori cells ( bmn ) and larvae of silkworms with baculovims expression vector system ( bevs ), to investigate the effect of human angiostatin ( kl - 3 ) on cell apoptosis in endothelial cells, and on vascular growth in chick embryo, and to elucidate cooperation between angiostatin and endostatin

    因此，在本實驗中我們用家蠶桿狀病毒表達系統表達了人血管抑素（ k1 - 3 ） ，並研究了其對內皮細胞凋亡和雞胚新生血管生長的影響，闡明了其與內皮抑素的協同作用。
  20. Inhibition of tumor - induced angiogenesis may represent an effective cytostatic strategy. angiostatin inhibits endothelial proliferation, angiogenesis, and tumor growth., so it is very important to receive a great deal of soluble, correctly folded, biologically ac

    Angiostatin已被證實是一種很具潛力的抗腫瘤生長和轉移的因子，因而若獲得大量具有高活性、高純度的angiostatin對腫瘤的治療會有重要的意義。
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