antibody enzyme 中文意思是什麼
antibody enzyme
解釋
抗體酶-
The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label
酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用，其靈敏度取決于標記酶的高度純化和高轉化率。 -
The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwich or competitive immunoreaction
將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。 -
The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwitch or competitive immunoreaction. the surface of immuno - sensor can be renewed by used in a new immunoassay
將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。 -
It was testified that the antibody can special immunological recognition the protein gst - cpti and cptl by indirect enzyme linked immunosorbent assay ( elisa ). the coefficient of correlation is significant and the potency is more than 1 : 800
經間接elisa法檢測，抗體能與gst - cpti 、 cpti蛋白特異性結合，其相關系數達到顯著水平，效價1 800 。 -
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質 -
In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。 -
Recently, there has been rapid development in the research and application of biosensor, which can detect analytes selectively using the specific reaction of bioactive materials such as the enzyme - substrate, enzyme - coenzyme, antigen - antibody, incretion - acceptor and so on
近年來，生物傳感器的研究和應用發展迅猛，它利用生物活性物質的親和性，如酶-底物、酶-輔基、抗原-抗體、激素-受體等的分子識別功能，可以有選擇地檢測待測物。 -
Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis. the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme. in studies, it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody. but it is difficult to obtain a single component by biochemical extraction. genetic engineering can help us to desolve this problem. after looking up mrna sequence encoding histidyl - trna synthetase in genebank, we used rt - pcr technology to gain its full length dna sequence. the vestor ptybllwas used in the construction of expressing vestor. we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen
本實驗從人胎盤中提取總rna ，利用rt - pcr技術獲得了編碼jo - 1的基因整長序列，選用impact - cn系統中的ptyb11載體，構建了jo - 1基因的克隆與表達載體，並轉化大腸桿菌er2566 ，經過抗性篩選、分子量大小比較、雙酶切鑒定、和pcr鑒定等多種方法驗證，篩選出了5個陽性克隆。 -
Molecular recognition is utilized to describe the molecular binding process in a specific manner. it ' s well known that the antibody / antigen, receptoraigand and enzyme / substrate are the three molecular recognition models in animate bodies
分子識別是分子間專一性的結合過程，抗體與抗原、受體與配體、酶與底物是人們所熟悉的生物體內的分子識別的三個模型。 -
The series of enzyme - labelled reagent kits the enzyme - labelled reagents adopt the enzyme - labelled monoclonal antibody technology, and have high sensitivity, excel distinctiveness and accurate results. a portion of kits can determine the amounts of antigen or antibody. the series widely apply to clinical diagnosis in hospital and general survey
酶標檢測試劑系列是採用單克隆抗體酶標記技術研製而成，具有靈敏度高特異性強結果準確等優點，部分產品還可定量檢測，廣泛適用於醫院臨床檢測和人群普查。 -
Methods a double mono - clonal antibody sandwich enzyme - linked immunosorbent assay ( elisa ) was used to detect the circulating antigens, antibodies and immune complex in 109 sera of neurocysticercosis
方法：用elisa法檢測109例腦囊尾蚴病患者血清抗原、抗體及循環免疫復合物。 -
Blood samples from 12, 000 hong kong citizens were collected and screened for the presence of the sars - cov antibody using enzyme - linked immunosorbent assay ( elisa ). positive samples underwent immunoflourescence assay ( ifa ) and western blot for confirmation
經初步酵素連結免疫吸附分析法（ elisa ）測試呈陽性的樣本更會透過免疫螢光法分析法（ immunofluorescenceassay ） ，及西方點墨法（ westernblot ）化驗以作最終確定抗體的存在。
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