antibody fragment 中文意思是什麼
antibody fragment
解釋
抗體片段-
The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method
Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。 -
Goat anti - human ige antibody were used as second antibody to make sure that the positive clones were ige related. through three cycles of screening, the inserted cdna fragments of the positive clones were amplified by pcr and sequenced. the results showed that the inserted cdna fragment from one clone was 1200 bp in length, with a orf of 507 bp which encoded 169 amino acids
Sj43b pgex 6p 1重組質粒的誘導表達、表達產物的鄉寸和免疫學性質鑒定分析為獲得可溶性的rsj43b月6gsta蟲合蛋白，對不同iptg誘導劑濃度、誘導表達溫度和誘導表達時間等因素對融合蛋白可溶性表達的影響進行了觀察。 -
In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。 -
The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis
取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。 -
The expressive gene fragment was 333bp long. analyses of the tcr y fragment shpwed that it contained three epitopes. it was showed that specific anti - idiotypic antibody could be found since four weeks after the first immunization and came to the climate on the sixth week. the antibody titers of the same time were higher in group pcdna3 / tcr y - il - 2 than in group pcdna3 / tcr y ( po. 01 ). the highest antibody titer was 1 : 640 in the group pcdna3 / tcr y - il - 2, whereas the highest was 1 : 160 in the group pcdna3 / tcr y. there were mrna expression in skeletal muscle cell could be found in group pcdnas / tcr y and group pcdna3 / tcr y - il - 2 on fifth day after inoculation
用tcryvi獨特型dna重組載體作為疫苗免疫小鼠，結果表明pcdna3汀cry組及pcdna3廳cry ? il ? 2組小鼠1血肩中全部產生了特異性抗獨特ffo抗體，抗體滴度在第4周開始增高，第6周時達到高峰。在同一取血時間， w兒1 ry一幾一二組小鼠抗體滴度均高於pcdna3 tcry組（ p 0 1 ） 。 pcd a3 tcry組小鼠抗體滴度最高達1 ： 16小pc 。 -
Spike protein was the main protective antigen inducing the neutralization antibody. the fragment containing antigenic sites b and c locating at the 5 " terminus in spike gene of tgev was deleted in prcv
Tgevs蛋白可誘導產生中和抗體，是主要的保護性抗原，其n端抗原位點b和c在prcv中缺失。 -
To answer this question, a bispecific, trifunctional antibody constructs which can not only target block virus incorporated rca, but also can induce complement activation by it ' s fc fragment were designed and constructed and iv the role of this kind of bispecific antibody in virus neutralization was studied. 1. to test our idea, human immunodeficient virus ( hiv ) and enveloped extracellular virus ( eev ) of vaccinia virus ( vv ) were selected in our study because of their complex immune evasion stratiges, their threaten to humans, and because both these two kinds of virus can escape complement attack by incorporating host rca into their envelope
以嚴重危害人類健康，且具有復雜免疫逃避機制的有包膜的hiv病毒及痘苗病毒的eev病毒為研究對象，首先對它們逃避補體攻擊的現象進行了驗證，探討了宿主膜補體調節蛋白cd55 、 cd59與hiv病毒及eev病毒免疫逃避的關系，評價了病毒結合的這兩種補體調節蛋白作為本研究提出的，通過消除病毒逃避補體攻擊的機制來恢復病毒對補體攻擊的敏感性，提高補體抗病毒效率這一抗病毒策略的靶點的可能性。 -
Conclusion : in this paper, we show huccr5 n - terminal gene fragment was cloned successfully and its specific antibody was prpared. by these we not only introduced a simple and quick method to get a specific antibody f ( ab ' ) 2of certain functional domain but also a good idea and technique to study other high similar superfamily members
中有效表達ccrsn端膜外第一謬基因片段，並制備出抗hllccrsn端的特異性抗體f （ ah 』 ） 。 。本方法為一簡捷快速的特定功能結構域抗體廠（ ah 』 ） 。
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