antigen binding 中文意思是什麼

antigen binding 解釋
抗原結合
  • antigen : n. 【醫學】抗原。
  • binding : adj 1 縛[捆、綁]…的；黏合的；系連的，連結的。2 有束縛力的，有拘束力的，附有義務的。3 〈口語〉引起...
  1. Detection of antigen - binding affinity of soluble mg7 scfv elisa was adopted to examine the antigen - binding affinity of soluble mg7 scfv ; competitive elisa was performed to test the inhibitory ratio of soluble mg7 scfv to the binding affinity of mg7 mab with its relevant antigen

    El舊a拐成惴現2個克隆的可容牲mg7seem明顯的抗原結合舌隊其a分別為0 832和0 912 ，均高出附性對照（ 0
  2. The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in

    實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。
  3. Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use

    X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。
  4. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  5. Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent

    方法根據抗原抗體特異性結合的原理，首先對抗a 、抗b單克隆抗體進行熒光標記，然後使熒光標記抗體與相應抗原（血痕）在最佳條件下結合，最後熒光顯微鏡鏡檢，判定血痕的血型。
  6. Dendritic cells ( dc ) is the most powerful apc, which can markedly increase the antigen - presentation capacity by maximizing the pepitide - mhc complexes on the cell surface and upregulating the co - stimulatory ligands b7 - 1 and b7 - 2, adhesion moleculees such as il - 12 that promote full activation of lymphocytes. full activation of antigen - specific t cells requires two signals - one signal coming via the tcr and the other signal through engagment of co - stimulatary molecules. t cells receiving one signal via their tcr are turned off by mhc ( major histocompatibility complex ), via t cell cd28 binding to b7 on the dc induce tlymphokine and t cell proliferatiion

    T細胞介導的細胞免疫在控制腫瘤生長方面發揮著重要作用， t細胞在發揮抗瘤效應（分泌細胞因子和直接殺傷）之前必須先經過活化，體內專職抗原提呈細胞（ apc ）細胞並使其活化，樹突狀細胞（ dendriticcell ， dc ）為t細胞的激活提供雙重信號， t細胞藉助tcr識別由dcmhc分子遞交的抗原肽后，通過tcr - cd3復合體傳遞抗原特異性識別信號（第一信號） ，以cd28為主的t細胞表面輔佐分子識別dc表面b7分子，傳遞非特異性協同刺激信號（第二信號） ，在機體抗腫瘤免疫應答中處于核心地位。
  7. Biological recognition with enzymes or antigen antibody binding are used to achieve high specificity

    酶的生物識別或抗原抗體結合使光纖傳感器可以獲得高的特異性。
  8. Effective activation of antigen - specific t cells not only requires the first signal transduction through t - cell receptor ( tcr ) binding with peptide - mhc complex on the antigen presenting cell ( apc ), but also needs the second signal, termed costimulation. costimulation critical to the degree and consequence of t cell activation is provided by interaction between soluble factors or cell - surface molecules on the t cell and on the apc

    而t細胞的活化除需要t細胞受體（ tcr ）與抗原呈遞細胞（ antigenpresentingcell ， apc ）表面的抗原肽- mhc復合物結合所形成的第一信號外，還需要t細胞和apc表面的其它膜分子結合所提供的共刺激信號（亦稱第二信號或輔助刺激信號， costimulatorysignal ）的參與。
  9. Because the antigen ? antibody reaction is an equilibrium binding reaction, the magnitude of the signal relies on the concentration of each reactant

    因為抗原抗體反應是一個平衡結合反應，所以信號量依賴于每個反應物的濃度。
  10. The binding reaction between antigen and antibody can be enhanced by optimizing chemical environments, such as acidity and ionic strength, to which the immunoreactants are exposed ( 34, 35 )

    抗原和抗體間的結合反應可通過優化免疫反應物暴露的化學環境，例如酸度和離子強度，來提高優化。
  11. One n - peptide - binding scfv clone was selected from the phage antibody library using affinity panning. the target antigen, n - peptide was biotinylated using photobiotin first

    N -肽是一種新發現的神經肽，其n端與阿片肽高度同源，可與阿片肽受體相互作用。
  12. The aim of this study was to evaluate the differences among oral vh, vc and scc by silver - binding nuclear organizer regions stain ( agnors ) and immunohistochemical staining for proliferating cell nuclear antigen ( pcna ), type iv collagen and laminin

    本研究目的希望藉由核仁組成區嗜銀蛋白染色，以及增殖細胞核抗原、第四型膠原蛋白及板層素之免疫組織化學染色，做為疣狀上皮增生、疣狀癌與鱗狀細胞癌之區別。
  13. Grp94 functions as molecular chaperones, which are ( poly ) peptide binding proteins that assist in protein folding, assembly and transport. moreover, its role of the transporter for antigen presentation has been discovered

    目前已知grp94的功能主要是作為分子伴侶，在新生肽鏈合成的早期階段，與新生肽鍵形成穩定的復合物，參與蛋白質的折疊、裝配和轉運。
  14. Cloning and expression of fragments of fibronectin to screen hepatitis b virus surface antigen - binding domains

    纖連蛋白的分段表達及與乙肝表面抗原相互作用研究
  15. Natural autoantibody ( naa ) refers to antibodies that are present in the surum of healthy individuals in the absence of deliberate immunization with the targeted antigen. previous studies have showed that human immunity system includes a huge and complicated autoantibody repetoire. to date, several hundred naa have been found and proved to exist in normal individuals, including autoantibody targeted rbc, mhc, dna, transferrin, keratin, diphosphatidyglycerd, idiotype of igg, etc. naa, characterized by binding to various and nonspecific antigen, reacting rapidly, keeping conservative relatively, has been widely claimed to be an important constituted component of innate immunity. lt was proposed that the production of naa was related to positive selection of b lymphocytes

    對naa的研究表明，健康人體免疫系統中存在著一個龐大而復雜的自身抗體網路。包括抗角蛋白自身抗體（ antikeratinautoantibody ， akautoab ）在內，迄今已經發現並證實的naa有數百種，如抗rbc 、 mhc 、 dna 、轉鐵蛋白、脂類和多糖的抗體以及jerne免疫調節網路中的抗獨特型抗體（ anti - idiotypeantibody ）等。由於個體出生即有naa ，且naa具有作用范圍廣、沒有特異的選擇性、反應出現快、有相對的穩定性等天然免疫的特點， naa被認為是天然免疫（ innateimmunity / naturalimmunity ）的重要組成部分。
  16. ( 4 ) in artificial pepsin digestive system with ph2. 0, due to the acid denaturation, the pigg was hydrolyzed wholly, in the system with ph3. 0 and 4. 0, although not all entirely pigg could be detected, but the pigg antibody activities against e. coli still existed without changing, this indicates that the f ( ab " ) 2 with antigen - binding activity still remain complete during digesting process

    （ 4 ）在ph2 . 0的人工胃液消化體系中，由於酸變性pigg全部水解；在ph3 . 0和ph4 . 0的人工胃液消化體系中，盡管檢測不到所有的pigg ，但pigg仍可與e . coli特異性結合且凝集價未發生變化，表明在消化過程中具有抗原結合活性的f （ ab ） _ 2片段仍可完整地保留下來。
  17. Molecular recognition is utilized to describe the molecular binding process in a specific manner. it ' s well known that the antibody / antigen, receptoraigand and enzyme / substrate are the three molecular recognition models in animate bodies

    分子識別是分子間專一性的結合過程，抗體與抗原、受體與配體、酶與底物是人們所熟悉的生物體內的分子識別的三個模型。
  18. Fortunately, recently widely used bispecific antibody technique provided us a good model for target rca blocking for it ' s dual antigen binding activity and for the availablity of high

    為了進一步探討用基因工程的方法制備抗病毒雙特異性抗體的可能性，我們又在現有的條件下構建、表達了
  19. It may be possible to obtain a single - chain antigen binding fragment smaller than the scfv

    重組克隆經酶切鑒定可見預期大小的片段，表明重組成功。
  20. After dissolving inclusion bodies, renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ), the fusion protein trx - scfv of electrophoretic purity was obtained, they still possessed antigen - binding activity

    經變性復性，用固相金屬離子親和色譜法一步分離純化了具有功能的單鏈抗體，在一升的發酵液中得到78mg的重組蛋白。
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