arabidopsis thaliana 中文意思是什麼

arabidopsis thaliana 解釋
阿拉伯芥
  1. It has been established the existence of a few pathways controlling flowering of plants in genetic research of flowering of arabidopsis thaliana and antirrhinum majus. many genes related to flowering have been identified and cloned, but the regulation mechanism of gene ' s expression is still unclear

    通過模式植物擬南芥和金魚草開花機理的研究,現在已經明確植物的開花途徑有多條,並識別和分離了許多與開花有關的基因,但這些基因如何在植物生長發育過程中進行特定時空的表達及其調控一直是科學家研究的一個黑匣子。
  2. In this experiment, seedlings of arabidopsis thaliana ( col ) were observed after being treated by verlicillium dahliae ( vd - toxin ), exogenous salicylic acid ( sa ), nitric oxide donor ( snp ) and nitric oxide synthase inhibitor ( nna ), then we investigated the changes of endogenous h2o2 content, the activity of the antioxidant enzymes catalase ( cat, ec : 1. 11. 1. 6 ) and ascorbate peroxidase ( apx, ec : 1. 11. 1. 11 ) and mrna levels of cat3 in different stress conditions, we also identified the localizations of h2o2 and no accumulated in the leaves of arabidopsis

    本實驗研究了棉花黃萎病菌?大麗輪枝菌毒素( vd - toxin )與擬南芥幼苗互作反應中外源sa 、 no供體snp 、 no合酶抑制劑nna等不同處理對擬南芥幼苗h _ 2o _ 2含量、 cat和apx活性及cat基因mrna表達量的影響,並對no 、 h _ 2o _ 2的積累部位進行染色檢測。
  3. Wild arabidopsis thaliana seeds were mutagenized with y - ray, and m2 populations were screened in the medium containing 200mmol / l nacl. 352 potential high - salt - tolerance mutants were selected from 80, 000 m2 seedlings, and 4 of this m2 mutants showed high - salt - tolerance in their m3 generation. this high - salt - tolerance mutants were backcrossed with the wild type arabidopsis thaliana

    在含200mmol lnacl的改良ms培養基上,通過對約80 , 000株m _ 2代幼苗的篩選,共獲得352株根仍表現向地性生長或真葉不變為褐色的植株作為可能的突變體。
  4. Genetic and mapping analysis of arabidopsis thaliana male sterile mutant ms1502 cruciferae

    1502的遺傳及定位分析
  5. Two primers, designed according to the conserved regions of ban gene in arabidopsis thaliana, were used to amplify the ban homologous fragments from the genomic dna of brassica napus, b. chinensisl, b. juncea, a. thaliana and other cultural plants of cruciferae. the very similar pcr fragments were obtained from all the amplifications, which implicated that ban may be a conserved gene existing widely in the genomes of cruciferae. pcr fragments were cloned and confirmed by restriction enzyme digestion

    參照擬南芥( arabidopsisthaliana ) ban基因與cdna設計引物,對甘藍型、白菜型、芥菜型油菜,擬南芥及其它十字花科栽培品種的基因組dna進行pcr擴增,均擴增出與擬南芥ban基因擴增片段大小極其相似的dna片段,提示ban可能廣泛存在於十字花科植物中。
  6. The materials in this study were arabidopsis thaliana. ( wt ) and its mutants including ethylene over producer mutant ( eto1 - 1 ), ethylene insensitive root mutant ( eir1 - 1 ) and ethylene insensitive mutant ( etr1 - 3 ). from the phenotypes of 12 - d - old seedlings of arabidopsis wt and mutants, the root gravitropism was obviously enhanced in etol - 1, but diminished in eir1 - 1 and etr1 - 3, compared with wt. the shoot negative gravitropism was also diminished in etiolated seedlings of etr1 - 3

    以擬南芥的eth過量產生型突變體( etol - 1 ) ,根對eth不敏感型突變體( eirl - 1 ,地上部對eth響應) , eth不敏感型突變體( etrl - 3 ,地上部和根均對eth不敏感)及其野生型( wt , columbia生態型)為實驗材料,利用根的向重力性反應為模式,分析了其中eth對auxin效應的調控及其原因。
  7. Under the stress of nacl lower than 100 mmol / l, the tissue structure of root had no distinct difference from the control. when the concentration of nacl was higher than 250 mmol / l, the stained color of safranine was deep but not even, and color in fibrovascular tissue and peripheral cortex cell were deeper than that around fibrovascular tissue. wild type arabidopsis thaliana and two salt - tolerance mutants were used for rapd analysis

    當nacl濃度小於100mmol l時,根組織結構與對照未表現出明顯的差異;但當nacl濃度達到250mmol l時,根組織結構對番紅染料的著色較深而且不均勻,維管組織和外圍皮層細胞著色較深,而維管組織周圍的細胞著色較淺。
  8. An improved method for indoor hydroponic culture of arabidopsis thaliana

    擬南芥室內水培方法的改進
  9. The study aimed to establish a simple, inexpensive, nearly - maintenanceless and flexible hydroponic system for growing arabidopsis thaliana plants by combining agar medium plus ms nutrients with the eppendorf tube system

    根據擬南芥的生長需求特點,採用ms培養基與營養液培養相結合的方法建立了一種簡單、低耗、低維護和靈活的擬南芥植株水培系統。
  10. Using the young seedlings of wild type ( wt ) and four mutants ( phya, phyb, phyaphyb and hy4 ) of arabidopsis thaliana as plant materials, the effects of light quality and kinetin ( kt ) on some photomorphogenesis responses such as cotyledon enlargement, hypocotyl growth inhibition, accumulation of chlorophyll and anthocyanin were investigated in the present study. photoreceptor functions and interaction of light and kt in the responses was examined and discussed

    本文以擬南芥( arabidopsisthaliana )野生型( wt )和四種光突變體phya 、 phyb 、 phyaphyb和砂hy4為材料研究不同光質與細胞分裂素( kt )對幼苗子葉擴大、下胚軸伸長、葉綠素和花色素苷積累的影響,分析光受體參與的反應以及探討光與kt在調節各種反應中的相互關系。
  11. Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively

    本實驗用sds - page電泳和免疫印跡的方法,用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定,結果表明:抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應,同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在,表明在植物中有類三磷酸肌醇受體蛋白的存在;用免疫膠體金方法,發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。
  12. Additionally, protein bands were found in the range of 62 kd in microsomes from oryza sativa and 45 kd from arabidopsis thaliana -, 2 ) the ipar - like protein was mainly localized in the vacuolar membrane ( tonoplast ) and plasma membrane in the leaf and root tip of arabidopsis thaliana for confirming the biological role of the ip3r - like protein, we investigated the effects of methyl viologen ( stimulating ip3 produce ) and heparin ( a competitive inhibitor of inositol 1, 4, 5 - triphophate in animal ) on stomatal movement by epidermal strip bioassay. when the epidermal strips of arabidopsis thaliana were treated with methyl viologen, it stimulates the stomatal closure

    擬南芥表皮條用甲基紫精處理,可以引起氣孔的關閉,當處理90分鐘的時候,氣孔的相對開度由100降到58 ,統計學分析氣孔開度的變化有顯著差異;甲基紫精引起氣孔關閉的作用可以被肝素部分抑制,當甲基紫精和肝素同時處理90分鐘的時候,氣孔的相對開度由100降到92 ,統計學分析氣孔開度的變化沒有顯著差異,說明氣孔的相對開度基本上沒有變化。
  13. We obtain two recessive monogenic salt - tolerance mutants from co60 - - mutagenized arabidopsis thaliana m2 populations. the effect of nacl on the structure of vegetative organa in arabidopsis thaliana was further studied and through a rapd analysis on salt - tolerance mutants of arabidopsis thaliana, a 1200bp dna fragment probably related to the salt - tolerance gene was get

    本實驗以模式植物擬南芥( arabidopsisthaliana )為材料獲得了隱性單基因抗鹽突變體,並研究了不同濃度的nacl脅迫對擬南芥營養器官結構的影響,進而通過對突變體的rapd分析,獲得了一個與植物抗鹽性有關的1200bp大小的基因片段。
  14. Gene engineering based on reducing na + toxicity creats a new approach to plant salt tolerance. this is the first time expressing of sod2 in arabidopsis thaliana and maybe attribute to research the ion homeostasis in the cell. the sod2 gene was isolated from s. pombe by rt - pcr method and confirmed by sequencing

    雖然sod2基因已經克隆,但是sod2在高等植物中異源表達及對其影響還未見報道,因此sod2基因在擬南芥中過量表達,對研究細胞的離子均衡及運用基因工程手段有效地提高植物的抗逆性具有重要意義。
  15. Asqs is 70 %, 77 %, 44 % and 39 % identical to squalene synthases from arabidopsis thaliana, tobacco, human and yeast, respectively. the asqs genomic dna has a complex organization containing 14 exons and 13 introns

    青蒿鯊烯合酶氨基酸序列與擬南芥、煙草、人類、酵母鯊烯合酶的一致性分別為70 、 77 、 44 、 39 。
  16. The ast ( anthocyanin spotted testa ) mutant, which was induced by carbon ion beam, was a single recessive gene mutant of arabidopsis thaliana and involved in the anthocyanin biosynthesis

    擬南芥ast ( anthocyaninspottedtesta )突變體是由碳離子束誘導產生的與花青苷生物合成有關的突變體,受單隱性核基因控制。
  17. Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha

    與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( e
  18. Plants are thought to remove na + from the cytoplasm by transporting it into the vacuolar or out of the cell using na + / h + exchangers localized in the vacuolar and plasma membranes, respectively. sos1 encoding a plasma membrane na + / h + antiporter and atnhxl encoding a vacuolar na + / h + antiporter were isolated from glycophytic arabidopsis thaliana, and overexpression of atnhxl and sos1 in arabidopsis thaliana increased the salt tolerance of transgenic plants significantly

    目前,擬南芥細胞內控制na ~ +外排的基因sos1及離子區隔化基因atnhx1均已克隆, sos1及atnhx1在擬南芥中的過量表達顯著提高了轉基因植株的耐鹽性,開創了降低na ~ +毒害的基因操作新途徑。
  19. The first two methods are the most commonly used for soybean transformation. the other methods have not been optimized for soybean, and are therefore less efficient and not often used. previously, we isolated ssnhxl ( encoding a vacuolar na + / h + antiporter ) from s. salsa, and further research was done through the overexpression in arabidopsis thaliana, it could help us to elucidate the salt tolerance of halophyte

    本實驗室成功的從鹽地堿蓬中克隆到位於液泡膜上的ndm 」反向轉運蛋白基因ssnhx ,轉化擬南芥並對該基因的功能進行了分析,發現在擬南芥中過量表兇亥基因可以巳著提;削6物的燦處性。
  20. H2o2 was cytochemically detected in the cells of epidermal hair and the cell wall of vascular parenchyma cell. cat and apx were the two major h2o2 - scavenging enzymes in plants. when seedlings of arabidopsis thaliana were treated by vd - toxin, the activity of cat and cat3 mrna level were decreased after sa treatment

    外源sa 、 no供體處理擬南芥幼苗均能誘導h _ 2o _ 2的積累, no供體的誘導作用最強; no合酶抑制劑處理則未表現出h _ 2o _ 2含量的增強: h _ 2o _ 2的積累部位主要在葉片的表皮毛和維管束組織。
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