arthrobacter 中文意思是什麼
arthrobacter
解釋
節核細菌屬-
The l - n - carbamoylase of arthrobacter bt801, coded by the hyuc gene, is the rate - limiting and the only stereoselective enzyme. hydantoin hydrolase gene ( hyuh ) and n - carbamoylase gene ( hyu c ) were amplified from the plasmid of puc18 - 169 by pcr and, at the same time, activity expression was obtained in e. coli and pichia pastor is gs115 respectively
節桿菌bt801的乙內酰脲水解酶催化乙內酰脲類化合物水解開環， n -氨甲酰基氨基酸水解酶是該菌乙內酰脲酶系中惟一具有立體選擇性的酶，也是整個反應體系的限速酶。 -
Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine. hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801. n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids
節桿菌（ arthrobacter ） bt801是由軍事醫學科學院生物工程研究所保存的l -乙內酰脲酶產酶菌株， puc18 - 169是由本室構建的含有節桿菌bt801的l -乙內酰脲酶完整操縱子序列的亞克隆質粒。 -
Multi - copies insertion transformants were screened on g418 plates. the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay. the n - carbamoylase activity of recombinant was 2. 26 and 2. 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169
將hyucdna片段連接到真核表達載體ppic3 . 5k上，經bgl酶切線性化，通過peg法轉化導入畢赤酵母gs115感受態細胞，利用g418抗性篩選得到12個插入多拷貝目的基因的轉化子。 -
Isolation and primary identification of a new nitrogng - fixation arthrobacter strain
一種有固氮能力的節桿菌菌株的分離和初步鑒定
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