as-pcr 中文意思是什麼

as-pcr 解釋
等位基因特異性多聚酶鏈反應
  • as : adv （同…）一樣…；同樣〈在此是指 as… as… 結構中的第一個 as 它在主句中為指示副詞，第二個 as （在子...
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  1. By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ), zymogram of 23 species of 11 genera in 4 subfamilies mirinae, orthotylinae, phylinae and deraeocorinae are gained. by specific primer pcr and sequencing, 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae, orthotylinae, phylinae, deraeocorinae, bryocorinae from 64 species of 23 genera which involved in this research, and 2 outgroup species of family anthocoridae as well. 1

    首次通過特異引物擴增和基因測序的研究方法從被試的5亞科23個屬的64種盲蝽中獲得了盲蝽亞科、合墊盲蝽亞科、葉盲蝽亞科、赤爪盲蝽亞科和蕨盲蝽亞科bryocorinae5個亞科17個屬的34種盲蝽以及外群花蝽科anthocoridae2種花蝽的cytb基因序列57條。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  4. The results are as following : 1 eric - pcr was for the first time applied in differentiating strains from edible fungi and proved to be more rapid and reliable than rapd in auricularia identification study. taken the similarity coefficient as 75 %, 29 strains of three auricularia species were grouped into 6 and 9 clusters by rapd and eric, respectively. eric - pcr clearly distinguished a. auricula from a. polytricha while rapd failed

    在75的分類水平上， eric - pcr把29個菌株分為9組，黑木耳種和毛木耳種可以明顯區分開；而rapd只能分為6組，不能將黑木耳和毛木耳分開，說明eric - pcr是比rapd更快捷可靠的分子標記，可以有效用於木耳屬的種質資源及遺傳分類的研究，也適合於其它食藥用菌種質資源的研究； 2
  5. By using primers designated for lacz gene, pcr result suggested an integration of lacz into u. pinnatifida genome. nine reagents including penicillin g, kanamycin, g418, teicoplanin, zeocin, chloramphinicol, hygromycin, basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes. the results showed that young sporophytes of u. pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers

    ，為了解決轉基因裙帶菜的篩選問題，進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗，包括抗生素類的青霉素g 、卡那黴素、 g418 、硫酸鏈黴素、鹽酸潔黴素、 zeocin 、氯黴素、潮黴素，除草劑類的basta ，氨甲喋呤類的氨甲喋呤，結果顯示裙帶菜幼孢子體對氯黴素、潮黴素敏感，對basta非常敏感。
  6. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  7. The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise

    本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物（ primer & primer ） ，以pbluescript ks +和puc18 / puc19質粒為載體，用常規的pcr方法從xanthomonascampestrispv . malvacearum （ smith ） dye等七株黃單胞菌（ xanthomomasspp . ）的基因組dna中克隆得到8個基因片段，分別命名為zhyf001 zhyf008 。
  8. Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control

    選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
  9. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  10. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  11. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  12. Primers were designed according to the sequences of atcal, bocal, bobcal and boical gene, and pcr amplifications were performed with the genomic dna of brussel sprouts, kale and kohlrabi respectively, pcr fragments of about 1. 6kb were obtained and designated correspondingly as bogcal5 ", boacal5 " and boccal5 "

    根據擬南芥atcal 、結球甘藍bocal 、花椰菜bobcal和青花菜boical基因設計引物，對蕓薹屬植物抱子甘藍、羽衣甘藍和球莖甘藍的基因組dna進行pcr擴增，均得到一段約1 . 6kb大小的pcr產物，分別命名為bogcal5 』 、 boacal5 』和boccal5 』 。
  13. The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

    利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。
  14. At first. then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr. following that, these gene fragments and plasmid vectors, pbluescript ii ks + and puc18, were cut by bamh i and kpn i. the prepared insert dna and vector dna were linked by t4 dna ligase

    利用vectornti6 . 0軟體，對所克隆的序列用相鄰接點法（ neighborjoining州j ） method ）進行多序列比對，分析其同源性，並構建基因進化樹。
  15. Used the genomic dna extracted by low melting - point agarose embedding method as pcr template, the full length of structural genes of bacillus subtilis bio operon were gained by long pcr method

    將該方法提取的基因組dna稀釋100倍作為模板，採用長距離pcr方法，獲得了枯草桿菌生物素操縱子基因全長。
  16. Among the three methods used in the experiment of dna extraction, only ctab, adding pvp in the dna isolation step, had effectively reduced the disturbance from fiber or other plastids and extracted suitable genomic dna as template for rapd process. pcr amplifications were performed in a final volume of 25 mm3 containing 0. 5 units taq polymerase, 2

    通過在抽提緩沖液加入2的pvp 、並用異丙醇和乙醇沉澱基因組dna等改良措施， ctab法能避開大量纖維、多糖的影響，有效地從不同屬種的棕櫚科植物的幼嫩葉片中提取並純化了適合rapd的基因組dna 。
  17. K01458, 1284bp ), we designed the primers and regard the pcr productions as the internal standard. then by using rt - pcr analysis, high levels of expression of emu ghrelin mrna were detectable in proventriculus were detected, low levels of expression of duck ghrelin mrna were also detectable in lung muscle ileum duodenum corpus striatum cerebellum brain stem and gizzard

    K01458 ， 1284bp ）設計引物，以其pcr產物作為內標，分別以鴯鶓各個組織反轉錄產物為模板，通過多重pcr的檢測ghrelin的mrna在鴯鶓的各個組織中表達情況，結果發現ghrelin的mrna在鴯鶓ghrelin的mrna除在腺胃特異表達外，在肺、肌肉、回腸、十二指腸、紋狀體、小腦、腦干、肌胃也有少量表達。
  18. Arc1, thl1 and thl2, the substrate protein genes of s receptor kinase, were cloned through a series of methods of molecular biology such as pcr, rt - pcr, dna cloning and sequencing. the resultings sequences were highly analysed by using the related biosoftwares on internet, providing new insights in the field of the molecular mechanism of self - incompatibility in plants. the major results are as followings : 1

    本文通過pcr和rt - pcr等一系列分子生物學方法克隆了蕓薹屬植物中的甘藍和油菜自交不親和信號傳導過程中srk底物蛋白基因arc1 、 thl1和thl2 ，並使用各種相關生物信息學軟體對srk底物蛋白基因序列進行了分析，然後在internet網上利用在線軟體對蛋白質的結構和功能進行了預測和探討，以期為蕓薹屬植物自交不親和性的分子機理的研究提供新的內容。
  19. The results of antigen substitution and antigen blocking experiments showed the aci - elisa has highly spectific reactivity with csfv. at last, the aci - elisa was used to test 30 samples, it yielded nearly the same results as pcr

    最後用aci - elisa與pcr對30份病料的檢測結果比較，表明aci - elisa與pcr檢測結果有很高的符合率。
  20. Three special bands as pcr products, obtained by using primer no. l, are reclaimed, and then used to cloning and sequencing. through homologous comparison with data from international nucleotide databank, the band ' s functions have been simply analyzed

    對1號簡並引物擴增出的3條特異性條帶進行了回收、克隆和測序；與國際核苷酸數據庫進行了同源性比較，對特異性片段的功能進行初步的分析和確定。
分享友人
分享到 Line

分享到臉書