aseptic method 中文意思是什麼
aseptic method
解釋
無菌操作法-
Although this method avoids the necessity of using aseptic techniqnes, as in the method of gilmore, we recommend that sterilized glassware be used.
雖然這種方法免去了應用滅菌技術的必要性，就像在Gilmore的方法中那樣，我們還是建議採用消毒的玻璃器皿。 -
Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture. the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group, while the epdermic cell were cultured simply as control. 24 hours later, under the invert microscope, the epidermic cells anchored well and transparent flat cells were observed in both groups. 7 days later, both cultured cells were taked out and fixed in 95 % ethanol, stained with hematoxylin and were observed under light microscope. many cleaved cells were observed in both groups. during cell culture, no pathogenic microganism was observed. so we considered the acellular dermal matrix was aseptic and had good biocompatibility. section three subdermal implantation of the acellular dermal matrix. 24 rats were used in the experiments. a piece of acellular dermal matrix ( 1. 5 x 1. 5cm2 ) was implanted beneath the dorsum skin flaps of each rat, 1 week, 2 weeks, 3 weeks and 4 weeks after implantation, 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured, the sections were used for he staining and observed under light microscope. the result were as folio wing : 1 - 2 weeks after implantation, the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation, the acellular dermal matrix adhered closely to the tissue around and could be recognized easily, 1 - 3 weeks after implantation, the size of implanted acellular dermal matrix had no statistical difference ( p > 0. 05 ). 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0. 05 ). under light microscope, l - 2weeks after implantation, the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix. 3 - 4 weeks after implantation, infiltrating blood vessels were evident. so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel
結果如下：皮下包埋卜周者，無細胞真皮基質漸與周圍組織粘附，顏色由蒼白轉紅；皮下包埋3周者，無細胞真皮基質與周圍組織緊密枯附，盾晰葉辯；術后卜周，包埋的基質面積變化較包埋前無統計學差異o川0引，術后4周包埋的無細胞真皮基質面積較包埋前縮小j刃刀5 ） 。光鏡下術后卜周，宿主的淋巳組織細胞、成纖維細胞浸入生長，釉附在膠原纖維上，少量血管內皮細胞浸入基質；術后34周，無細胞真皮基質內較多的血管形成，故可認為無細胞真皮基質免疫原性低，能誘導宿主的成纖維細胞、巨噬細胞浸入生長，為一種新型的真皮替代物。第四部分無細胞真皮基質與自體斷層皮片復合移棺的研究， sd大鼠10隻，在其背部卜方造成全厚皮膚缺損的創面 -
Although this method avoids the necessity of using aseptic techniqnes, as in the method of gilmore, we recommend that sterilized glassware be used
雖然這種方法免去了應用滅菌技術的必要性，就像在gilmore的方法中那樣，我們還是建議採用消毒的玻璃器皿。
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