badh 中文意思是什麼

badh 解釋
巴德
  1. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  2. We isolated the badh cdna ( acbadh ) from the halophyte atriplex centralasiatica iljin using rt - pcr and the deduced protein sequences showed high similarity with other plants

    我們以鹽生植物中亞濱藜（ atriplexcentralasiaticailjin ）為材料，用pt - pcr方法克隆了badhcdna 。
  3. Up to date, the cauliflower mosaic virus ( camv ) 35s promoter and its derivatives are used commonly. no report show transgenic plants expressed badh from halophyte and using its own promoter or other strong promoters

    目前在遺傳工程中所用的啟動子多為35s ，還沒有高效脅迫誘導啟動子的實際運用的報道。
  4. Badh gene extends 7. 7kb containing 15 exons with the open reading frame of sooaa, 1. 2kb putative promoter region

    該基因全長7774hp ，包含15個外顯子， 14個內含子以及約1
  5. In higher plants, betaine is synthesized by a two - step oxidation of choline : choline - betaine aldehyde - betaine. the first step is catalyzed by choline monooxygenase ( cmo ). the second step is catalyzed by betaine aldehyde dehydrogenase ( badh )

    高等植物中，甜菜堿由膽堿經兩步酶催氧化得到，即：膽堿- -甜菜堿醛- -甜菜堿，催化第一步反應的酶是膽堿單加氧酶（ cholinemonooxygenase ， cmo ） ，催化第二步反應的酶是甜菜堿醛脫氫酶（ betainealdehydedehydrogenase ， badh ） 。
  6. Badh cdna ( 1901bp ) included a 66 bp 5 " utr, a 329 bp 3 " utr and a 1506 bp orf encoding a 501 - ammo - acid polypeptide which showed 88 % sequence identity to badh from spinach, sugar beet and atriplex hortensis respectively. the deduced amino acid sequence included a decapeptide sequence " vtlelggksp ", which is highly conserved among general aldehyde dehydrogenases ( aldh ), and a cysteine residue

    Badhcdna全長1901bp ， 5端非編碼區66bp ， 3端非編碼區329bp ，含有2個可能的加polya信號： aataa ，開放閱讀框架1506bp ，編碼一個由501個氨基酸構成的多肽，與菠菜、甜菜、山菠菜badh的氨基酸序列同源性均為88 ，其中有醛脫氫酶的保守序列vtlelggksp和半胱氨酸殘基。
  7. According to reference, this research is the first time to transfer practically productive aim - gene to tetraploid black locust, and it is also the first time to transfer badh gene to forest - tree. this research provide a useful way in improving resistance to soline - alkali and drought of forest tree

    據資料檢索，該研究屬首次將具有生產意義的目的基因導入四倍體刺槐，也是首次將甜菜堿醛脫氫酶基因導入林木樹種，在培育耐鹽抗旱型林木品種方面進行了有益的探索。
  8. In this research project, the agrobacterium tumefaciens mediated transformation of badh gene of tetraploid black locust has been studied, for the purpose of improving resistance to soline - alkali and drought of tetraploid black locust ; of playing more important role in developing of waste lands, ameliorating of soline - alkali soil, and greening and beautifying of surface - mined lands, mine waste dumps, slopes of roads and railroads where restoration of vegetative cover has proven difficult ; of fully making benefits of its ability to fix atmospheric nitrogen in the soil

    為了進一步提高四倍體刺槐的耐鹽性和抗旱性，進一步擴大其適宜種植的生態范圍，充分發揮其固氮、改良土壤的特性，在我國的城鎮綠化、荒山造林、鹽堿地改良以及采礦跡地、公路、鐵路邊坡等植物生長困難土地的植被恢復中發揮其優勢，本實驗對四倍體刺槐進行了農桿菌介導的甜菜堿醛脫氫酶基因轉化的研究。
  9. The purified badh, which became a single band in sds - page, has been obtained by a combination of ammonium sulfate fractionation, ion exchange chromatography and gel filtration chromatography

    遼寧堿蓬甜菜堿醛脫氫酶分子量為129kd ，單個亞基的分子量為64 . 5kd ，表明其為同型二聚體。
  10. The library consisted of 1. 3 x 106 clones with an average insert size of about 18kb. the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin. screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr, we got four positive clones

    3x10 『個重組噬菌體，插入片段大小約為18kb ，含插入片段的頻率為100隊以中亞濱蓉甜菜堿醛脫氫酶門adh ）基因近5 』端的約400hp片段為探針，篩選中亞濱蓉基因組文庫，得到了4個陽性克隆。
  11. 89 ) via agrobacterium mediation. pcr and southern blotting analysis showed that the cmo and badh genes were integrated into tobaccos " genome. betaine accumulation in transformed tobaccos was higher than that in control plants

    89 ） ，獲得卡那黴素抗性和潮黴素抗性植株， pcr和southern雜交均證明外源cmo和badh基因大連理工大學博士學位已整合到煙草基因組中。
  12. Many scientists have introduced badh gene to plants and got transgenic plants which overexpressed badh, but the accumulations of glybetaine are too low to function effectively

    許多研究者已得到了轉badh基因植物，雖然轉基因植物中badh的表達量提高，但是甘氨酸甜菜堿的積累濃度並不高。
  13. One of them contained the entire badh genomic sequences

    其中第4號克隆包含了badh基因的全序列。
  14. The transformed tobacco with badh gene can survive on ms medium containing 1. 2 % nacl

    5 naci的培養基中正常生長，轉badh基因能在含1
  15. The cmo and badh genes were transferred into tobaccos ( nictiana tabacum l. cv

    農桿菌介導法將cmo和badh基因分別導入煙草（ nictianatabacuml
  16. However, the exact mechanisms of transcriptional activation of the badh gene leading up to such activation events remain poorly understood

    Badh基因受脅迫誘導表達，但是對它轉錄調控的分子機制還不清楚。
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