binding affinity 中文意思是什麼
binding affinity
解釋
結合親和力-
The binding affinity of remaining hemoglobin sites for oxygen is changed by the presence of co binding.
氧在血紅蛋白其餘位置上的鍵合力被一氧化碳所改變。 -
Detection of antigen - binding affinity of soluble mg7 scfv elisa was adopted to examine the antigen - binding affinity of soluble mg7 scfv ; competitive elisa was performed to test the inhibitory ratio of soluble mg7 scfv to the binding affinity of mg7 mab with its relevant antigen
El舊a拐成惴現2個克隆的可容牲mg7seem明顯的抗原結合舌隊其a分別為0 832和0 912 ，均高出附性對照（ 0 -
The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。 -
The recombinant gloshedobin binding to the affinity adsorbent con a indicated that it is a glycoprotein
此重組類凝血酶在37t中性溶液中保存過夜將分解成小肽，但在0t下很穩定。 -
The high binding affinity and selectivityin conjunction with the polymers ' physical robustness positions molecular imprinted polymers ( mips ) as candidates for use as preliminary screens in drug discovery
聚合物的高親和力、高選擇性及物理性質穩定特點使得分子印跡聚合物可用於藥物開發中的初篩。 -
Correspondingly, the egf binding affinity of surface egfr and the tyrosine auto - phosphorylation of immuno - precipitated egfr were also enhanced in gntv - s / h7721 cells and reduced in gntv - as / h7721 cells
Egfr的信號通路主要有兩條，一是ras - raf - mek一mapk經典途徑，另一條是新發現的pi一3k一pkb途徑。 -
Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。 -
The experimental method includes selecting pure complexes of histidine - containing or cysteine - containing materials, from c - and n - terminal group of these amino acids to link to a group which have color or fluorescence or ultraviolet absorption, elucidating their binding affinity, fluorescence or uv - visible spectrum properties with zinc at physiological concentration and to elucidate their structure in the solid state via infrared spectroscopy. with the help of the concerned the data, the analysis was done to prove whether it can be applied to the zinc detection, in other words, whether it can be used as a new fluorescence probe for zinc detection
本實驗首次選用在生物體內與zn ~ （ 2 + ）鍵合能力很突出的物質? ?組氨酸和半胱氨酸，採用類似於多肽合成的方法，在其羧基或氨基分別嫁接上一個帶有標記的基團，生成穩定的共價鍵化合物；在此化合物中模擬生理濃度條件加入鋅離子，通過紅外圖譜、紫外圖譜或熒光圖譜的變化分析鋅離子對標記基團是否產生影響，再結合有關數據分析其是否適合檢測鋅離子，即是否可能作為新的鋅離子熒光探針。 -
Reports about great enthusiasm in the synthesis of the complexes of rare earth medicine, the interaction of the rare earth complexes with dna are very few in the world. it is expected to explore the mechanisms of interaction that are used to offer an opportunity to understand which parameters affect the binding mode and binding affinity to dna. part ii
從這些總結中了解到，人們之所以選擇蘆丁作為小分子配體，是由於蘆丁所具有的特性：它屬于黃酮類化合物，具有獨特的藥用價值及其臨床應用價值，更令人興奮的是它還具有良好的電活性，這樣就可以利用電化學方法來研究其作用機理。 -
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。 -
Gc binds to mr with higher affinity than gr. this gc - receptor interaction is also controlled at the pre - receptor level by two important factors : corticosteroid binding globulin ( cbg ) and 11 b - hydroxysteroid dehydrogenases ( 11 - hsds )
糖皮質激素與受體結合的量除與血漿中糖皮質激素水平、皮質醇結合蛋白（ cbg ）濃度有關外，還受到細胞內受體前糖皮質激素代謝酶? 11 -羥基類固醇脫氫酶（ 11 - hsd ）的調節。 -
A novel chitosan ( cts ) - binding protein was isolated from the non - heading chinese cabbage leaves by chitosan affinity chromatography and partially characterized
說明cts在調控氨同化關健酶gdh和gs活性時，可能有ca 』 +信號系統的參與。 -
Part i this paper has minutely studied the interaction between ag ( i ) and serum albumin. the binding of ag ( i ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 5. 4 ). the scatchard analysis indicates that there exists several strong binding sites of ag ( i ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of ag ( i ) with hsa or bsa using uv - visible spectrometry at ph ( 5. 4 ), which shows that the binding between ag ( i ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for ag ( i ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has been also studied by resonance light - scattering spectrum ( rls ) and flurescence quenching
第一部分：等離子點ph （ 5 . 4 ）條件下，用平衡透析法和紫外光譜，熒光光譜，共振散射光譜研究了ag （ ）與人血清白蛋白（ humanserumalbumin ，簡稱hsa ）或牛血清白蛋白（ bovineserumalbumin ，簡稱bsa ）的結合。 scatchard圖分析表明， ag （ ）在hsa或bsa中有強弱兩類結合部位，通過計算機擬合獲得結合的逐級穩定常數值。紫外掃描發現ag （ ）與hsa或bsa的結合存在滯後效應，表明ag （ ）與hsa或bsa的結合可能誘導蛋白質構象發生緩慢變化（ a - b ） ，測得並討論了這一構象變化的速度常數和活化參數。 -
Calbindin d - 28k ( cb ) is an important member of calcium - binding protein family and has a high affinity for binding ca - ", one of the important intracellular messengers. the cb has been regarded as a selective mark er of some neuronal populations especially of projection neurons in various regions of the central nervous system
Calbindind - 28k （ cb ）是生物體內一種與鈣離子具有較高親合力的蛋白質，作為中樞神經系統一些部位內神經元特別是投射神經元的特異性標識物被廣泛應用。 -
One n - peptide - binding scfv clone was selected from the phage antibody library using affinity panning. the target antigen, n - peptide was biotinylated using photobiotin first
N -肽是一種新發現的神經肽，其n端與阿片肽高度同源，可與阿片肽受體相互作用。 -
Recently an abscisic acid binding protein with high specificity and affinity for aba was purified from epidermis of vicia faba l. in our laboratory, and further characterized using various biochemical techniques, there is evidence to link the protein to the physiological effects of aba in some aspects. the above - mentioned work may result in the molecular cloning the gene encoding aba binding protein
近年來，我室利用親和層析從蠶豆下表皮中分離出一種高親合、高度特異性的aba結合蛋白，並完成了對其生化特性深入分析及部分測序，且在一定程度上揭示了該蛋白與aba的生理功能效應存在著內在的聯系，這為進一步克隆aba結合蛋白基因奠定了堅實的工作基礎。 -
Part ii this paper has minutely studied the interaction between au ( iii ) and serum albumin. the binding of au ( iii ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 7. 43 + 0. 02 ). the scatchard analysis indicates that there exists several strong binding sites of au ( iii ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of au ( iii ) with hsa or bsa using uv - visible spectrometry at ph ( 7. 43 + 0. 02 ), which shows that the binding between au ( iii ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for au ( iii ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has project supported by the national science foundation of china ( no. 299621001 ), and the foundation for talents of the ten hunred thousand in guangxi
第二部分：生理ph （ 7 . 43 0 . 02 ）條件下，用平衡透析法和紫外光譜，熒光光譜研究了金（ ）與人血清白蛋白（ humanserumalbumin ，簡稱hsa ）或牛血清白蛋白（ bovineserumalbumin ，簡稱bsa ）的結合。 scatchard圖分析表明，金（ ）在hsa或bsa中有強弱兩類結合部位，通過計算機擬合獲得結合的逐級穩定常數值。利用熒光猝滅法計算出金（ ） - hsa和金（ ） - bsa體系的stem - volmer常數和雙分子猝滅速率常數。 -
The screening on affinity ligand of heart - type fatty acid - binding protein
心肌型脂肪酸結合蛋白特異性親和配體的篩選 -
A sepharose - heparin column ( a affinity chromatoraphic column ) was used to study the binding of heparin to protein previously. in the 1990 ' s, a sensitive technique developed for the study of the potential interaction of cytokine with heparin and related polysaccharides is affinity coelectrophoresis ( ace )
以往主要是通過sepharose - heparin親和層析來研究肝素與蛋白的相互作用； 20世紀90年代又發展了affinity - coelectrophoresis （ ace ）技術研究蛋白質與肝素的相互作用。 -
The specificity and affinity and antibody are the key points of antibody based immunotherapy for target andtibody binding and for expected effects
為此，我們首先制備、純化了免抗eev病毒抗體及小鼠抗人cd55的抗體。
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