biochemical technology of protein 中文意思是什麼
biochemical technology of protein
解釋
蛋白質生物化學技術- biochemical : adj. 生物化學的。n. 生物化學物質。adv. -ly
- technology : n. 1. 技術，工程，工藝。2. 製造學，工藝學。3. 術語（匯編）。
- of : OF ＝Old French 古法語。
- protein : n. 【化學】朊，蛋白（質）。
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Serials of papain products : papain is a protein hydrolysis enzyme which extracted & purified from papaya latex by advanced biochemical technology
木瓜酶系列產品：木瓜酶是利用現代生物工程技術從未成熟番木瓜乳汁中提取出來的一種蛋白水解酶。 -
Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis. the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme. in studies, it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody. but it is difficult to obtain a single component by biochemical extraction. genetic engineering can help us to desolve this problem. after looking up mrna sequence encoding histidyl - trna synthetase in genebank, we used rt - pcr technology to gain its full length dna sequence. the vestor ptybllwas used in the construction of expressing vestor. we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen
本實驗從人胎盤中提取總rna ，利用rt - pcr技術獲得了編碼jo - 1的基因整長序列，選用impact - cn系統中的ptyb11載體，構建了jo - 1基因的克隆與表達載體，並轉化大腸桿菌er2566 ，經過抗性篩選、分子量大小比較、雙酶切鑒定、和pcr鑒定等多種方法驗證，篩選出了5個陽性克隆。
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