biological assay 中文意思是什麼

biological assay 解釋
生物測定,生物學鑒定
  • biological : adj. 生物學（上）的。 a biological test 生物學檢驗。n. 【藥學】生物製品，生物制劑。adv. -ly 生物學地，生物學上。
  • assay : n 1 化驗；分析；鑒定，測定，驗定。2 被分析物，被化驗物。3 化驗結果，化驗報告。4 〈古語〉企圖，嘗...
  1. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  2. 14 out of 25 strains of biocontrol agent streptomyces showed chitinolytic activity by biological assay. these 14 strains of biocontrol agent streptomyces were detected for chitinase gene using pcr primers derived from the conservative sequence of 17 cloned chitinase genes from streptomyces

    此方法相對于幾丁質酶的傳統生物學檢測，具有更好的準確性和靈敏性；而且與生物學檢測相比，使用分子檢測省時省力。
  3. Biological activity was determined by egf dependent balb / c 3t3 cell line and with mtt colorimetric assay. extracts of the recombinant virus - infected and mock - infected cells, haemolymph of the recombinant virus infected and mock - infected silkworm larvae could all support the proliferation of balb / c 3t3 cell. this phenomena implied that there were some egf - like growth factors in the haemolymph of normal silkworm larvae, which could enhance the proliferation of the cell line

    用小鼠balb c3t3成纖維細胞和mtt法測定表達產物的促細胞增殖作用，發現重組病毒感染家蠶細胞72小時的胞內樣品與正常家蠶細胞裂解物，以及重組病毒感染4天的蠶血淋巴與正常蠶血淋巴均具有相似的促細胞增殖作用，甚至野生型病毒感染的細胞裂解物和蠶血淋巴也有一定的細胞促生長作用，提示家蠶系統本身可能含有能促進細胞生長、類似於egf的細胞因子。
  4. Biological assay proved that the expressed product could stimulate the proliferation of cd34 + hematopoietic cells. conclusion : flt - 3 ligand extracellular domain was successfully expressed

    體外活性實驗表明，畢赤氏酵母表達的重組fl蛋白可以有效地刺激造血幹細胞cd34 「的增殖。
  5. Application of fluorescence resonance energy transfer of quantum dots in biological assay

    量子點的熒光共振能量轉移在生物分析中的應用
  6. The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target, lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt. the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1. 6 0. 1 104 the dosage of amd was lug / ml, and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution. the credibility of the established system was detected with rhtnfp developed by r & d

    為確定經上述步驟純化后得到的目的蛋自lt 27的生物活性，本研究以l929細胞為靶細胞、淋巴毒素國際標準品為參照，採用結晶紫染色法檢測經淋巴毒素處理后存活的細胞，對淋巴毒素生物活性測定的細胞接種濃度、淋巴毒素標準品板上稀釋的起始濃度和梯度稀釋的倍數、放線菌素d的使用劑量等進行條件實驗后，建立了人淋巴毒素生物活性測定方法。
  7. Expression of a novel human interleukin - 13 in e. coli and its biological activity assay

    13在大腸桿菌中的表達及生物活性分析
  8. Biological assay is one of important fields in life science

    生物分析是生命科學中一個重要的領域。
  9. The biological activity of purified lt 27 was tested with the assay system, and its biological activity was 2 - 3 107 iu / mg. pro. the cytotoxicity of purified lt 27 was in the same level with rhtnfp and lt international standard. it shows that lt deletion could keep its high cytotoxicity towards tumour cell l929 in vitro after 27 amino acids deleted from its n - terminal

    用建立的淋巴毒素生物活性測定方法對上述純化的淋巴毒素缺失體lt 27的生物活性進行檢測，測得其比活為2一3xl口iu / mg . proo純化的淋巴毒素缺失體lt 27的生物活性與rhtnfp和淋巴毒素國標標準品的生物活性大致相當，表明lt經n端缺失27個氨基酸后仍能保持很高的體外腫瘤細胞毒活性。
  10. To study the biological function of sh2a gene, we constructed its recombinant expression vec - tor, and investigated its function by cell transfection, kinase assay, subcellular localization and expression analysis. materials and methods 1

    我們構建了sh _ 2a基因的真核重組表達載體，通過細胞轉染、激酶活性檢測、亞細胞定位、表達分析和流式細胞儀等方法對其功能進行了初步的研究。
  11. The researchers added a third test, the tibia line rat growth assay, to detect the biological action of the hormones, a novel approach to the study of growth hormones in exercise

    研究者加入了第三種方法，脛骨線生長反應，以檢測激素的生物學行為，是在訓練中研究生長激素的新方法。
  12. In our experiments, choosing the model plant, arabidopsis thaliana as the target of low - energy ion implantation, we observed and counted the biological effects and studied the genetic characters of some varied plants using rapd assay, to make the fundaments for exploring the interactions between low - energy ions and complicated organisms further

    本研究特選擇模式植物擬南芥為實驗材料進行低能離子處理，觀察和統計了離子注入所引起的的生物學效應，並利用rapd技術對部分變異株進行了遺傳分析，旨在為進一步探索低能離子與生物體相互作用規律的研究打下基礎。
  13. Assay of the biological characteristics of transgenic sgc

    轉基因克隆株的生物學特性鑒定
  14. Multi - copies insertion transformants were screened on g418 plates. the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay. the n - carbamoylase activity of recombinant was 2. 26 and 2. 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169

    將hyucdna片段連接到真核表達載體ppic3 . 5k上，經bgl酶切線性化，通過peg法轉化導入畢赤酵母gs115感受態細胞，利用g418抗性篩選得到12個插入多拷貝目的基因的轉化子。
  15. Biological evaluation of dental materials. part 2 : biological evaluation test method of dental materials. salmonella typhimurium reverse mutation assay ames mutagenicity test

    口腔材料生物學評價.第2單元：口腔材料生物試驗方法.鼠傷寒沙門氏桿菌回復突變試驗ames試驗
  16. Now the biological and molecular assays are the major methods to assay cpti transgenic plant. there is no better method on assaying the expression of the transgene. the reason would be that the outcome of expression by cpti is a little peptide and its molecular weight is very small. its immu - nogenicity would be insufficiency so that it can not induce strong immu - nological reaction and can not produce special antibody of high potency

    目前對轉cpti基因植物的檢測主要集中在生物測定和分子檢測，尚無較好的基因表達產物的檢測方法，其主要原因在於cpti基因的表達產物是小分子多肽，分子量小，免疫原性不夠，直接免疫不足以產生較強的免疫反應，不能產生效價高的特異性抗體。
分享友人
分享到 Line

分享到臉書