biosoftwares 中文意思是什麼
biosoftwares
解釋
生物軟體-
Arc1, thl1 and thl2, the substrate protein genes of s receptor kinase, were cloned through a series of methods of molecular biology such as pcr, rt - pcr, dna cloning and sequencing. the resultings sequences were highly analysed by using the related biosoftwares on internet, providing new insights in the field of the molecular mechanism of self - incompatibility in plants. the major results are as followings : 1
本文通過pcr和rt - pcr等一系列分子生物學方法克隆了蕓薹屬植物中的甘藍和油菜自交不親和信號傳導過程中srk底物蛋白基因arc1 、 thl1和thl2 ，並使用各種相關生物信息學軟體對srk底物蛋白基因序列進行了分析，然後在internet網上利用在線軟體對蛋白質的結構和功能進行了預測和探討，以期為蕓薹屬植物自交不親和性的分子機理的研究提供新的內容。 -
Firstly, the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector. comparing the eo sequences with other strains eo by biosoftwares, dnastar5. 0 and bioedit, the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii. two amino acid streches of 15 csfv strains eo, which form the rnase active site, and histidine residues essential for rnase catalysis in both ones were highly conserved. the eo protein propertys of antigen epitope, hydrophobicity, isoelectric point were also predicted by bioinformatic method
首先，採用rt - pcr和nest - pcr技術擴增了csfv - jl株和csfv - ln9912株eo基因，插入teasy載體后測序，應用dnastar5 . 0和bioedit軟體將其與本實驗室已測其它毒株和genebank中登錄的csfv代表毒株eo基因序列進行比較，繪制了遺傳進化樹並預測了eo蛋白的抗原表位、疏水性、等電點等特性。
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