biotin-labeled 中文意思是什麼
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Labeled avidin biotin method, lab method
生物素法 -
The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification
本文回顧了李壞死環斑病毒的檢測方法，較全面地評述了國內外病原物檢測技術研究進展：在模板的擴增有各種pcr技術、 nasba技術；在信號的放大有同位素、生物素或dig標記的cdna和crna探針，分支探針和肽核酸探針；模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術；模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。 -
Surface plasmon resonance immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years. however, a major disadvantage of spr for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly. therefore, it is a challenging task to develop strategies for improving the detection limit sensitivity of spr. in this paper, authors present a novel strategy for improving the sensitivity of spr immunosensing using streptavidinbiotinylated antibody complex. it is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity. this amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. the complex can be formed in a crosslinking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex
將鏈霉親和素-生物素系統用於表面等離子體共振免疫傳感的信號放大，實時檢測了人免疫球蛋白g higg的蛋白濃度。發生免疫反應的傳感片和生物素化抗體反應后，傳感片表面的一層生物素分子隨后與鏈霉親和素-生物素化抗體復合物中的鏈霉親和素的活性位點發生親和反應，從而使傳感片表面特異健合的物質質量顯著增加，大大提高了免疫檢測的靈敏度和檢測限。免疫反應經放大后，可檢測0 . 00510g ml濃度區間內的higg 。 -
Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a. biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction
抗體通過單層蛋白a固定到金電極表面，預先處理好的生物素化抗體和辣根過氧化物酶（ hrp ）標記的親和素形成的網狀復合物作為免疫反應信號的放大探針。
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