chain sequence 中文意思是什麼

chain sequence 解釋
鏈順序
  • chain : n 1 鏈子，鏈條；項圈；表鏈。2 連鎖；連續，一系列，一連串；（山）脈。3 〈常 pl 〉鐐銬；羈絆，拘束...
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  1. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  2. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  3. G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software

    本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。
  4. In this paper, 45 e. coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e. coli by animal test. type 1 pili of 45 strains isolated was detected by msha. the pila gene of 45 avian pathogenic e. coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank. results showed that pcr was more sensitive, faster and more characteristic than msha to detect type 1 pili

    本研究將從四川規模化雞場分離鑒定、經1日齡雛雞致病性試驗得到的雞源致病性大腸桿菌45株，採用d -甘露糖敏感血凝試驗（ msha ）檢測1型菌毛，根據genbank中公布的人源大腸桿菌1型菌毛pila基因序列設計一對引物用pcr擴增雞源致病性大腸桿菌1型菌毛pila基因。
  5. To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative

    目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。
  6. Morphological observations and rbcl sequence analyses of chondrus collected from pingtan and zhangpu of fujian province, qingdao of shandong province and dalian of liaoning province were made. total dna were extracted from fresh or silica gel - desiccated plants and used as templates for polymerase chain reactions

    在第四部分，採用rbcl序列分析結合傳統形態觀察的方法，對我國福建省平潭縣和漳浦縣、山東省青島市及遼寧省大連市採集的角叉菜屬樣品進行了研究。
  7. A 40 - base polymorphic repeat sequence located in the 3 ' - untranslated region of the dat gene was purified and amplified by polymerase chain reaction ( pcr )

    將位於多巴胺運輸器基因3 '端未轉譯區段的40 -堿基多形性重復序列予以純化、經聚合酶鏈鎖反應放大。
  8. This thesis focuses on techniques of dynamic fault tree in system reliability modeling and its qualitative and quantitative analysis. it studies bdd solution for static sub trees 、 markov chain solution for dynamic sub tree briefly and the modularization of dynamic fault tree ; presents the algorithm for top event occurrence rate of dynamic fault tree based on weibull distribution. then this thesis presents a new approach to solve top event occurrence rate and a new generation algorithm of minimal cut sequence of dynamic fault tree that deviate from markov model completely

    本文著眼于動態故障樹在系統可靠性建模及定性定量分析中的技術，研究了基於bdd的靜態子樹分析方法、基於馬爾可夫模型的動態子樹分析方法以及動態故障樹模塊化方法，並提出了基於威布爾分佈的動態故障樹頂事件發生概率計算方法;提出了一種完全脫離馬爾可夫模型的求解動態故障樹頂事件發生概率的方法和一種最小順序割集的生成方法。
  9. It is a very basic, single - chain protein with molecular weight about 14 kd. it shares 33 % sequence identity with bovine pancreatic rnasea and has structurally equivalent counterparts for the two histidines and one lysine that comprise the catalytic residues for ribonucleolytic activity

    它是由123個氨基酸組成的分子量約為14kd的單鏈堿性蛋白質，與牛胰核糖核酸酶有33的同源性，兩個組氨酸和一個賴氨酸殘基組成了血管生成素核糖核酸酶活性中心。
  10. The electrons are transferred along the chain to a carrier molecule ( coenzyme q ), and then in sequence to a series of cytochromes, finally acting with the enzyme cytochrome oxidase to reduce an oxygen atom, which combines with two h + ions to form water

    電子沿呼吸鏈傳遞到載體分子? ?輔酶q上，然後依次經過一系列細胞色素分子的傳遞，最後與細胞色素氧化酶反應，氧原子結合兩個氫形成水從而被還原。
  11. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  12. 4. correspond to the characteristic of computer aided dimensional chain calculation, present the judgment of redundancy equations, judgment of increase - decrease chrematistic, auto assign of unknown, algorithm of equations sequence calculation, enhance the robust and the calculation succeed proportion of the software

    （ 4 ）針對尺寸鏈方程的計算機求解特點，提出了冗餘方程的判斷及刪除準則、組成環增減性判斷優化值法、未知數自動賦初值、方程組順序求解等演算法，大大提高了計算的成功率和軟體的健壯性。
  13. In order to use the responding ability to the inducers of pr - la promoter, two fragments, ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank. sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence. its transcription start site, tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter

    根據pr - 1a基因的報道序列，設計兩對引物，以煙草基因組為模板，通過pcr擴增得到900bp （ ipl ）和603bp （ ips ）兩個目標片段，序列測定表明克隆的啟動子與報道序列具有99的同源性，轉錄起始位點、 tatabox及保守序列tgac與報道序列均完全相同。
  14. A pair of primers were designed according to the published sequence of gnrh2 ' s gene mrna and transporter gene with oligo version 4. 1 softwarre, they were annealed by their 3 ' terminal brief complementary base sequence to form small segment double chain, therery, they serve mutually as template and primer, and their extension were carried out to synthesize 90 bp " gnrh / trs

    本研究根據genbank中已發表的人gnrh2基因mrna序列以及轉運肽（ transporter ， trs ）基因核苷酸序列，藉助oligo4 . 1設計了一對寡核苷酸引物，以引物3末端的短互補序列退火形成小段雙鏈，從而互為模板和引物，通過引導合成長達90bp的gnrh trs序列gnrh trs 。
  15. This paper presents the conversion from dynamic logic gate to markov chain, the solution of dynamic subtree top event failure probability and the method of obtaining the failure mode of subsystem using markov model, that is sequence cutsets of the dynamic subtree. the typical approach to importance analysis of component is impractical for large systems in markov model, so this paper also provides a simple and intuitionistic graph solution based on markov chain

    論文研究了動態邏輯門向馬爾可夫鏈的轉化方法，利用馬爾可夫鏈法求解動態子樹頂事件概率，以及通過馬爾可夫狀態轉移圖直接找齣子系統的故障模式和薄弱環節，即得到動態子樹的順序割集。
  16. The ability to schedule transmissions and chain a sequence of polls in a single command is included

    另外還包括了傳輸調度和單一命令中鏈接輪詢序列的能力。
  17. If you recover the database after restoring one of the intermediate transaction log backups, that before the end of the log chain, you cannot restore the database past that point without restarting the complete restore sequence, starting with the full database backup

    如果要在還原其中一個中間事務日誌備份之後恢復數據庫，則在日誌鏈結束之前，除非從完整數據庫備份開始重新啟動整個還原順序，否則，將無法還原該點之前的數據庫。最好的做法就是在恢復數據庫之前還原所有的事務日誌，然後再另行恢復數據庫。
  18. The comparison of meq gene sequences of different pathotypes of marek ' s disease virus meq genes of different pathotypes of marek ' s disease virus ( mdv ) were amplified, in the whole opening reading frame ( orf ) by polymerase chain reaction ( pcr ) technique, sequenced and compared with the published sequence of ga strain ( representing vmdv ). cvi988 / rispens and 814, commercial vaccine strains popularly used worldwide and in china respectively ; 648a, representing very virulent plus ( vv + mdv ) and 6 field isolates, originated from guangxi commercial chickens with visceral lymphomas and vaccine breaks, representing high pathogenic ( hpmdv ) and two of them ( g2 and n ) were proved to be vvmdv, were used

    本研究通過三個方面進行了研究，取得了如下主要實驗結果： 1mdv不同致病型（ pathotypes ）毒株meq基因序列的比較研究應用pcr技術擴增了不同致病型mdvs ，即國際通用型疫苗毒cv1988 rispens株和中國特有的疫苗毒814株、特超強毒648a株（ vv + mdv ）以及6個廣西分離到的野毒株共9個毒株的meq基因，進行核苷酸序列的測定並與標準強毒ga株（ vmdv ）進行核苷酸和氨基酸序列的比較。
  19. 4. the seguence of cloned endostatin gene was confirmed with the dideoxy chain - termination method and is consistent with reported sequence of mouse endostatin

    4 .以雙脫氧末端終止法對重組質粒pbv220一endostatin進行測序，結果表明與己知小鼠endostatin基因序列一致。
  20. Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected

    Rapd （隨機擴增多態性dna ）技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術，它建立於聚合酶鏈式反應（ pcr ）技術基礎之上，利用隨機合成的寡聚核苷酸序列為引物（一般為10個bp ） ，分別與dna的兩條單鏈結合，在dna聚合酶的作用下，對基因組的特定區域進行pcr擴增，其電泳結果為不同大小和數目的dna譜帶即rapd圖譜，可反映基因組相應區域的dna多態性。
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