choline treatment 中文意思是什麼
choline treatment
解釋
膽堿療法-
Mechanism of glutamate - induced inhibition of pulmonary surfactant lipid synthesis ? mda receptor ( nmdar ) antagonist mk - 801 could reverse glutamate - induced inhibition of [ 3h ] - choline incorporation in lung explants ; ? he expression of nmdar1 subtype existed in alveolar type ii epithelial cells ; ? he effect of glu on pc synthesis required l - arginine in cultured lung explants, nos activity and no production were increased significantly in lung explants with glu treatment ; and mk - 801 and nos inhibitor l - nna could block the increase of nos activity and no production induced by glu ; l - nna also could reverse the inhibition of [ 3h ] - choline incorporation induced by glu ; ( 4 ) mda content increased and sod level decreased in cultured lung explants of glu treament groups, mk - 801 and l - nna could reverse these effects ; ( 3 ) mk - 801, l - nna could block the release of ldh induced by glu
谷氨酸對肺表面活性物質脂質合成抑制的機制研究nmda受體拮抗劑mk - 801能逆轉glu所致肺組織[ ~ 3h ] -膽堿摻入減少的效應；肺泡上皮細胞有nmda受體nmdar1亞單位的表達； glu對ps脂質合成的影響依賴l -精氨酸的存在， glu可使肺組織勻漿中nos活性、 no合成量升高； mk - 801 、 nos的抑制劑l - nna均可阻斷glu所致的這些效應； l - nna可逆轉glu所致肺組織[ ~ 3h ] -膽堿摻入減少的效應； glu可使肺組織勻漿中mda碩士學位論文中文摘要含量增高、 sod水平下降。 mk七0 、 l nna能阻斷q所致的這些效應； mk80 、 l nna還能阻斷gill誘導的ldh釋放增多的效應。 -
Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。
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